Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy

Abstract Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary. We propose to use polarization-dependent fluorescence correlation spectroscopy (Pol-FCS) for evalu...

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Autores principales: Johtaro Yamamoto, Akito Matsui, Fusako Gan, Makoto Oura, Riku Ando, Takahiro Matsuda, Jian Ping Gong, Masataka Kinjo
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/53c4c8b0214b46eba997354324be71f7
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