Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation

Pasteurella multocida is one of the primary pathogens of bovine respiratory disease (BRD), and causes huge losses in the cattle industry. The Pm3 strain was a natural isolate, which is a strong form of pathogen and is sensitive to fluoroquinolones antibiotics. A high fluoroquinolone resistant strain...

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Autores principales:	Li Zhan, Jiaqi Zhang, Boyu Zhao, Xintian Li, Xiqing Zhang, Renge Hu, Emad Mohammed Elken, Lingcong Kong, Yunhang Gao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	P. multocida serogroup A complete genome sequencing transcriptomic sequencing gene islands virulence gene Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/53c7c2fc30f84434b1c7aa10fc04078f
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spelling	oai:doaj.org-article:53c7c2fc30f84434b1c7aa10fc04078f2021-11-10T08:28:35ZGenomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation2297-176910.3389/fvets.2021.765495https://doaj.org/article/53c7c2fc30f84434b1c7aa10fc04078f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.765495/fullhttps://doaj.org/toc/2297-1769Pasteurella multocida is one of the primary pathogens of bovine respiratory disease (BRD), and causes huge losses in the cattle industry. The Pm3 strain was a natural isolate, which is a strong form of pathogen and is sensitive to fluoroquinolones antibiotics. A high fluoroquinolone resistant strain, Pm64 (MIC = 64 μg/mL), was formed after continuous induction with subinhibitory concentration (1/2 MIC) of enrofloxacin, with the enhanced growth characteristics and large attenuation of pathogenicity in mice. This study reports the whole genome sequence and the transcription profile by RNA-Seq of strain Pm3/Pm64. The results showed an ineffective difference between the two strains at the genome level. However, 32 genes could be recognized in the gene islands (GIs) of Pm64, in which 24 genes were added and 8 genes were lost. Those genes are involved in DNA binding, trehalose metabolism, material transportation, capsule synthesis, prophage, amino acid metabolism, and other functions. In Pm3 strain, 558 up-regulated and 568 down-regulated genes were found compared to Pm64 strain, from which 20 virulence factor-related differentially expressed genes (DEGs) were screened. Mainly differentially transcribed genes were associated with capsular polysaccharide (CPS), lipopolysaccharide (LPS), lipooligosaccharide (LOS). Iron utilization, and biofilm composition. We speculated that the main mechanism of virulence attenuation after the formation of resistance of Pm64 comes from the change of the expression profile of these genes. This report elucidated the toxicity targets of P. multocida serogroup A which provide fundamental information toward the understanding of the pathogenic mechanism and to decreasing antimicrobial drugs resistance.Li ZhanJiaqi ZhangBoyu ZhaoXintian LiXiqing ZhangRenge HuEmad Mohammed ElkenLingcong KongLingcong KongYunhang GaoYunhang GaoFrontiers Media S.A.articleP. multocida serogroup Acomplete genome sequencingtranscriptomic sequencinggene islandsvirulence geneVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	P. multocida serogroup A complete genome sequencing transcriptomic sequencing gene islands virulence gene Veterinary medicine SF600-1100
spellingShingle	P. multocida serogroup A complete genome sequencing transcriptomic sequencing gene islands virulence gene Veterinary medicine SF600-1100 Li Zhan Jiaqi Zhang Boyu Zhao Xintian Li Xiqing Zhang Renge Hu Emad Mohammed Elken Lingcong Kong Lingcong Kong Yunhang Gao Yunhang Gao Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation
description	Pasteurella multocida is one of the primary pathogens of bovine respiratory disease (BRD), and causes huge losses in the cattle industry. The Pm3 strain was a natural isolate, which is a strong form of pathogen and is sensitive to fluoroquinolones antibiotics. A high fluoroquinolone resistant strain, Pm64 (MIC = 64 μg/mL), was formed after continuous induction with subinhibitory concentration (1/2 MIC) of enrofloxacin, with the enhanced growth characteristics and large attenuation of pathogenicity in mice. This study reports the whole genome sequence and the transcription profile by RNA-Seq of strain Pm3/Pm64. The results showed an ineffective difference between the two strains at the genome level. However, 32 genes could be recognized in the gene islands (GIs) of Pm64, in which 24 genes were added and 8 genes were lost. Those genes are involved in DNA binding, trehalose metabolism, material transportation, capsule synthesis, prophage, amino acid metabolism, and other functions. In Pm3 strain, 558 up-regulated and 568 down-regulated genes were found compared to Pm64 strain, from which 20 virulence factor-related differentially expressed genes (DEGs) were screened. Mainly differentially transcribed genes were associated with capsular polysaccharide (CPS), lipopolysaccharide (LPS), lipooligosaccharide (LOS). Iron utilization, and biofilm composition. We speculated that the main mechanism of virulence attenuation after the formation of resistance of Pm64 comes from the change of the expression profile of these genes. This report elucidated the toxicity targets of P. multocida serogroup A which provide fundamental information toward the understanding of the pathogenic mechanism and to decreasing antimicrobial drugs resistance.
format	article
author	Li Zhan Jiaqi Zhang Boyu Zhao Xintian Li Xiqing Zhang Renge Hu Emad Mohammed Elken Lingcong Kong Lingcong Kong Yunhang Gao Yunhang Gao
author_facet	Li Zhan Jiaqi Zhang Boyu Zhao Xintian Li Xiqing Zhang Renge Hu Emad Mohammed Elken Lingcong Kong Lingcong Kong Yunhang Gao Yunhang Gao
author_sort	Li Zhan
title	Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation
title_short	Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation
title_full	Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation
title_fullStr	Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation
title_full_unstemmed	Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation
title_sort	genomic and transcriptomic analysis of bovine pasteurella multocida serogroup a strain reveals insights into virulence attenuation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/53c7c2fc30f84434b1c7aa10fc04078f
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Genomic and Transcriptomic Analysis of Bovine Pasteurella multocida Serogroup A Strain Reveals Insights Into Virulence Attenuation

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