<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles

ABSTRACT mRNA profiling of pathogens during the course of human infections gives detailed information on the expression levels of relevant genes that drive pathogenicity and adaptation and at the same time allows for the delineation of phylogenetic relatedness of pathogens that cause specific diseas...

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Autores principales:	Piotr Bielecki, Uthayakumar Muthukumarasamy, Denitsa Eckweiler, Agata Bielecka, Sarah Pohl, Ansgar Schanz, Ute Niemeyer, Tonio Oumeraci, Nils von Neuhoff, Jean-Marc Ghigo, Susanne Häussler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/53cc49bb2bf54caab4b3f0087e7fd30c
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spelling	oai:doaj.org-article:53cc49bb2bf54caab4b3f0087e7fd30c2021-11-15T15:47:22Z<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles10.1128/mBio.01075-142150-7511https://doaj.org/article/53cc49bb2bf54caab4b3f0087e7fd30c2014-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01075-14https://doaj.org/toc/2150-7511ABSTRACT mRNA profiling of pathogens during the course of human infections gives detailed information on the expression levels of relevant genes that drive pathogenicity and adaptation and at the same time allows for the delineation of phylogenetic relatedness of pathogens that cause specific diseases. In this study, we used mRNA sequencing to acquire information on the expression of Escherichia coli pathogenicity genes during urinary tract infections (UTI) in humans and to assign the UTI-associated E. coli isolates to different phylogenetic groups. Whereas the in vivo gene expression profiles of the majority of genes were conserved among 21 E. coli strains in the urine of elderly patients suffering from an acute UTI, the specific gene expression profiles of the flexible genomes was diverse and reflected phylogenetic relationships. Furthermore, genes transcribed in vivo relative to laboratory media included well-described virulence factors, small regulatory RNAs, as well as genes not previously linked to bacterial virulence. Knowledge on relevant transcriptional responses that drive pathogenicity and adaptation of isolates to the human host might lead to the introduction of a virulence typing strategy into clinical microbiology, potentially facilitating management and prevention of the disease. IMPORTANCE Urinary tract infections (UTI) are very common; at least half of all women experience UTI, most of which are caused by pathogenic Escherichia coli strains. In this study, we applied massive parallel cDNA sequencing (RNA-seq) to provide unbiased, deep, and accurate insight into the nature and the dimension of the uropathogenic E. coli gene expression profile during an acute UTI within the human host. This work was undertaken to identify key players in physiological adaptation processes and, hence, potential targets for new infection prevention and therapy interventions specifically aimed at sabotaging bacterial adaptation to the human host.Piotr BieleckiUthayakumar MuthukumarasamyDenitsa EckweilerAgata BieleckaSarah PohlAnsgar SchanzUte NiemeyerTonio OumeraciNils von NeuhoffJean-Marc GhigoSusanne HäusslerAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 4 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Piotr Bielecki Uthayakumar Muthukumarasamy Denitsa Eckweiler Agata Bielecka Sarah Pohl Ansgar Schanz Ute Niemeyer Tonio Oumeraci Nils von Neuhoff Jean-Marc Ghigo Susanne Häussler <italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles
description	ABSTRACT mRNA profiling of pathogens during the course of human infections gives detailed information on the expression levels of relevant genes that drive pathogenicity and adaptation and at the same time allows for the delineation of phylogenetic relatedness of pathogens that cause specific diseases. In this study, we used mRNA sequencing to acquire information on the expression of Escherichia coli pathogenicity genes during urinary tract infections (UTI) in humans and to assign the UTI-associated E. coli isolates to different phylogenetic groups. Whereas the in vivo gene expression profiles of the majority of genes were conserved among 21 E. coli strains in the urine of elderly patients suffering from an acute UTI, the specific gene expression profiles of the flexible genomes was diverse and reflected phylogenetic relationships. Furthermore, genes transcribed in vivo relative to laboratory media included well-described virulence factors, small regulatory RNAs, as well as genes not previously linked to bacterial virulence. Knowledge on relevant transcriptional responses that drive pathogenicity and adaptation of isolates to the human host might lead to the introduction of a virulence typing strategy into clinical microbiology, potentially facilitating management and prevention of the disease. IMPORTANCE Urinary tract infections (UTI) are very common; at least half of all women experience UTI, most of which are caused by pathogenic Escherichia coli strains. In this study, we applied massive parallel cDNA sequencing (RNA-seq) to provide unbiased, deep, and accurate insight into the nature and the dimension of the uropathogenic E. coli gene expression profile during an acute UTI within the human host. This work was undertaken to identify key players in physiological adaptation processes and, hence, potential targets for new infection prevention and therapy interventions specifically aimed at sabotaging bacterial adaptation to the human host.
format	article
author	Piotr Bielecki Uthayakumar Muthukumarasamy Denitsa Eckweiler Agata Bielecka Sarah Pohl Ansgar Schanz Ute Niemeyer Tonio Oumeraci Nils von Neuhoff Jean-Marc Ghigo Susanne Häussler
author_facet	Piotr Bielecki Uthayakumar Muthukumarasamy Denitsa Eckweiler Agata Bielecka Sarah Pohl Ansgar Schanz Ute Niemeyer Tonio Oumeraci Nils von Neuhoff Jean-Marc Ghigo Susanne Häussler
author_sort	Piotr Bielecki
title	<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles
title_short	<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles
title_full	<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles
title_fullStr	<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles
title_full_unstemmed	<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles
title_sort	<italic toggle="yes">in vivo</italic> mrna profiling of uropathogenic <named-content content-type="genus-species">escherichia coli</named-content> from diverse phylogroups reveals common and group-specific gene expression profiles
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/53cc49bb2bf54caab4b3f0087e7fd30c
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<italic toggle="yes">In Vivo</italic> mRNA Profiling of Uropathogenic <named-content content-type="genus-species">Escherichia coli</named-content> from Diverse Phylogroups Reveals Common and Group-Specific Gene Expression Profiles

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