Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis

Abstract Liver cancer is the fatal cause of cancer deaths worldwide due to its aggressiveness and lack of effective therapies. Tiliroside (C30H26O13) is an active compound extracted from herb plant Tribulus terrestris L., which has been used as alternative therapy in clinic practice. However, its th...

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Autores principales:	Rui Han, Hongxing Yang, Lingeng Lu, Lizhu Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53d6f466d7634f228da7883493ea5ad2
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spelling	oai:doaj.org-article:53d6f466d7634f228da7883493ea5ad22021-12-02T18:27:50ZTiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis10.1038/s41598-021-88133-72045-2322https://doaj.org/article/53d6f466d7634f228da7883493ea5ad22021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88133-7https://doaj.org/toc/2045-2322Abstract Liver cancer is the fatal cause of cancer deaths worldwide due to its aggressiveness and lack of effective therapies. Tiliroside (C30H26O13) is an active compound extracted from herb plant Tribulus terrestris L., which has been used as alternative therapy in clinic practice. However, its therapeutic use against liver cancer has not been previously reported. Here, we showed that Tiliroside exerted significantly higher anti-proliferation effect on liver cancer cell lines Hep3B and SNU-449 than on liver normal cell THLE-3 cells or NC group, respectively, by using MTS assay. Results from colony formation, immigration and invasion assays support the anticancer efficacy of Tiliroside and its low-toxic property while treating liver normal cell THLE-3. 3D spheroid formation and CD133 expression level also displays its anti-stemness effect. It has been showed that Tiliroside may function as Carbonic anhydrases XII (CAXII) inhibitor and affects apoptotic E2F1/E2F3/Caspase-3 axis by using CAXII esterase activity assay, Human carbonic anhydrase 12 (CA-12) ELISA Kit, quantitative reverse transcription PCR (RT-qPCR) as well as CaspACE Assay System, respectively. In summary, we demonstrate for the first time that Tiliroside suppresses liver cancer development possibly by acting as a novel CAXII inhibitor, which warrant further investigation on its therapeutic implications.Rui HanHongxing YangLingeng LuLizhu LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rui Han Hongxing Yang Lingeng Lu Lizhu Lin Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis
description	Abstract Liver cancer is the fatal cause of cancer deaths worldwide due to its aggressiveness and lack of effective therapies. Tiliroside (C30H26O13) is an active compound extracted from herb plant Tribulus terrestris L., which has been used as alternative therapy in clinic practice. However, its therapeutic use against liver cancer has not been previously reported. Here, we showed that Tiliroside exerted significantly higher anti-proliferation effect on liver cancer cell lines Hep3B and SNU-449 than on liver normal cell THLE-3 cells or NC group, respectively, by using MTS assay. Results from colony formation, immigration and invasion assays support the anticancer efficacy of Tiliroside and its low-toxic property while treating liver normal cell THLE-3. 3D spheroid formation and CD133 expression level also displays its anti-stemness effect. It has been showed that Tiliroside may function as Carbonic anhydrases XII (CAXII) inhibitor and affects apoptotic E2F1/E2F3/Caspase-3 axis by using CAXII esterase activity assay, Human carbonic anhydrase 12 (CA-12) ELISA Kit, quantitative reverse transcription PCR (RT-qPCR) as well as CaspACE Assay System, respectively. In summary, we demonstrate for the first time that Tiliroside suppresses liver cancer development possibly by acting as a novel CAXII inhibitor, which warrant further investigation on its therapeutic implications.
format	article
author	Rui Han Hongxing Yang Lingeng Lu Lizhu Lin
author_facet	Rui Han Hongxing Yang Lingeng Lu Lizhu Lin
author_sort	Rui Han
title	Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis
title_short	Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis
title_full	Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis
title_fullStr	Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis
title_full_unstemmed	Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis
title_sort	tiliroside as a caxii inhibitor suppresses liver cancer development and modulates e2fs/caspase-3 axis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/53d6f466d7634f228da7883493ea5ad2
work_keys_str_mv	AT ruihan tilirosideasacaxiiinhibitorsuppresseslivercancerdevelopmentandmodulatese2fscaspase3axis AT hongxingyang tilirosideasacaxiiinhibitorsuppresseslivercancerdevelopmentandmodulatese2fscaspase3axis AT lingenglu tilirosideasacaxiiinhibitorsuppresseslivercancerdevelopmentandmodulatese2fscaspase3axis AT lizhulin tilirosideasacaxiiinhibitorsuppresseslivercancerdevelopmentandmodulatese2fscaspase3axis
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Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis

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