Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding

Rieu et al. present a magnetic tweezers based single-molecule manipulation method, called kinetic locking, for direct detection of biomolecular binding without use of fluorescent probes. By measuring dynamical interactions of E. coli RecQ helicase with its DNA substrate, authors show that this metho...

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Main Authors:	Martin Rieu, Jessica Valle-Orero, Bertrand Ducos, Jean-François Allemand, Vincent Croquette
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Biology (General) QH301-705.5
Online Access:	https://doaj.org/article/53d9b151442c49a496d43795d732b58f
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spelling	oai:doaj.org-article:53d9b151442c49a496d43795d732b58f2021-12-02T15:16:05ZSingle-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding10.1038/s42003-021-02606-z2399-3642https://doaj.org/article/53d9b151442c49a496d43795d732b58f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02606-zhttps://doaj.org/toc/2399-3642Rieu et al. present a magnetic tweezers based single-molecule manipulation method, called kinetic locking, for direct detection of biomolecular binding without use of fluorescent probes. By measuring dynamical interactions of E. coli RecQ helicase with its DNA substrate, authors show that this method holds promise for studying DNA-DNA and DNA-protein interactions while avoiding the need for labelling.Martin RieuJessica Valle-OreroBertrand DucosJean-François AllemandVincent CroquetteNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Martin Rieu Jessica Valle-Orero Bertrand Ducos Jean-François Allemand Vincent Croquette Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
description	Rieu et al. present a magnetic tweezers based single-molecule manipulation method, called kinetic locking, for direct detection of biomolecular binding without use of fluorescent probes. By measuring dynamical interactions of E. coli RecQ helicase with its DNA substrate, authors show that this method holds promise for studying DNA-DNA and DNA-protein interactions while avoiding the need for labelling.
format	article
author	Martin Rieu Jessica Valle-Orero Bertrand Ducos Jean-François Allemand Vincent Croquette
author_facet	Martin Rieu Jessica Valle-Orero Bertrand Ducos Jean-François Allemand Vincent Croquette
author_sort	Martin Rieu
title	Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
title_short	Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
title_full	Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
title_fullStr	Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
title_full_unstemmed	Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
title_sort	single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/53d9b151442c49a496d43795d732b58f
work_keys_str_mv	AT martinrieu singlemoleculekineticlockingallowsfluorescencefreequantificationofproteinnucleicacidbinding AT jessicavalleorero singlemoleculekineticlockingallowsfluorescencefreequantificationofproteinnucleicacidbinding AT bertrandducos singlemoleculekineticlockingallowsfluorescencefreequantificationofproteinnucleicacidbinding AT jeanfrancoisallemand singlemoleculekineticlockingallowsfluorescencefreequantificationofproteinnucleicacidbinding AT vincentcroquette singlemoleculekineticlockingallowsfluorescencefreequantificationofproteinnucleicacidbinding
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Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding

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