Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.

Coxiella burnetii, the causative agent of the zoonotic disease Q fever, is mainly transmitted to humans through an aerosol route. A spore-like form allows C. burnetii to resist different environmental conditions. Because of this, analysis of the survival strategies used by this bacterium to adapt to...

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Autores principales:	Quentin Leroy, Kevin Lebrigand, Fabrice Armougom, Pascal Barbry, Richard Thiéry, Didier Raoult
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53da8361f1684876ad8e1d15cd5a1868
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spelling	oai:doaj.org-article:53da8361f1684876ad8e1d15cd5a18682021-11-18T07:01:24ZCoxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.1932-620310.1371/journal.pone.0015321https://doaj.org/article/53da8361f1684876ad8e1d15cd5a18682010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21203564/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Coxiella burnetii, the causative agent of the zoonotic disease Q fever, is mainly transmitted to humans through an aerosol route. A spore-like form allows C. burnetii to resist different environmental conditions. Because of this, analysis of the survival strategies used by this bacterium to adapt to new environmental conditions is critical for our understanding of C. burnetii pathogenicity. Here, we report the early transcriptional response of C. burnetii under temperature stresses. Our data show that C. burnetii exhibited minor changes in gene regulation under short exposure to heat or cold shock. While small differences were observed, C. burnetii seemed to respond similarly to cold and heat shock. The expression profiles obtained using microarrays produced in-house were confirmed by quantitative RT-PCR. Under temperature stresses, 190 genes were differentially expressed in at least one condition, with a fold change of up to 4. Globally, the differentially expressed genes in C. burnetii were associated with bacterial division, (p)ppGpp synthesis, wall and membrane biogenesis and, especially, lipopolysaccharide and peptidoglycan synthesis. These findings could be associated with growth arrest and witnessed transformation of the bacteria to a spore-like form. Unexpectedly, clusters of neighboring genes were differentially expressed. These clusters do not belong to operons or genetic networks; they have no evident associated functions and are not under the control of the same promoters. We also found undescribed but comparable clusters of regulation in previously reported transcriptomic analyses of intracellular bacteria, including Rickettsia sp. and Listeria monocytogenes. The transcriptomic patterns of C. burnetii observed under temperature stresses permits the recognition of unpredicted clusters of regulation for which the trigger mechanism remains unidentified but which may be the result of a new mechanism of epigenetic regulation.Quentin LeroyKevin LebrigandFabrice ArmougomPascal BarbryRichard ThiéryDidier RaoultPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15321 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Quentin Leroy Kevin Lebrigand Fabrice Armougom Pascal Barbry Richard Thiéry Didier Raoult Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
description	Coxiella burnetii, the causative agent of the zoonotic disease Q fever, is mainly transmitted to humans through an aerosol route. A spore-like form allows C. burnetii to resist different environmental conditions. Because of this, analysis of the survival strategies used by this bacterium to adapt to new environmental conditions is critical for our understanding of C. burnetii pathogenicity. Here, we report the early transcriptional response of C. burnetii under temperature stresses. Our data show that C. burnetii exhibited minor changes in gene regulation under short exposure to heat or cold shock. While small differences were observed, C. burnetii seemed to respond similarly to cold and heat shock. The expression profiles obtained using microarrays produced in-house were confirmed by quantitative RT-PCR. Under temperature stresses, 190 genes were differentially expressed in at least one condition, with a fold change of up to 4. Globally, the differentially expressed genes in C. burnetii were associated with bacterial division, (p)ppGpp synthesis, wall and membrane biogenesis and, especially, lipopolysaccharide and peptidoglycan synthesis. These findings could be associated with growth arrest and witnessed transformation of the bacteria to a spore-like form. Unexpectedly, clusters of neighboring genes were differentially expressed. These clusters do not belong to operons or genetic networks; they have no evident associated functions and are not under the control of the same promoters. We also found undescribed but comparable clusters of regulation in previously reported transcriptomic analyses of intracellular bacteria, including Rickettsia sp. and Listeria monocytogenes. The transcriptomic patterns of C. burnetii observed under temperature stresses permits the recognition of unpredicted clusters of regulation for which the trigger mechanism remains unidentified but which may be the result of a new mechanism of epigenetic regulation.
format	article
author	Quentin Leroy Kevin Lebrigand Fabrice Armougom Pascal Barbry Richard Thiéry Didier Raoult
author_facet	Quentin Leroy Kevin Lebrigand Fabrice Armougom Pascal Barbry Richard Thiéry Didier Raoult
author_sort	Quentin Leroy
title	Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
title_short	Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
title_full	Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
title_fullStr	Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
title_full_unstemmed	Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
title_sort	coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/53da8361f1684876ad8e1d15cd5a1868
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Coxiella burnetii transcriptional analysis reveals serendipity clusters of regulation in intracellular bacteria.

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