Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

Abstract Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pouria Fattahi, Ali Rahimian, Michael Q. Slama, Kihak Gwon, Alan M. Gonzalez-Suarez, Jadon Wolf, Harihara Baskaran, Caden D. Duffy, Gulnaz Stybayeva, Quinn P. Peterson, Alexander Revzin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/53e0a7662aba4e918a6dcc5423f60520
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:53e0a7662aba4e918a6dcc5423f60520
record_format	dspace
spelling	oai:doaj.org-article:53e0a7662aba4e918a6dcc5423f605202021-12-02T13:26:32ZCore–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor10.1038/s41598-021-85786-22045-2322https://doaj.org/article/53e0a7662aba4e918a6dcc5423f605202021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85786-2https://doaj.org/toc/2045-2322Abstract Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.Pouria FattahiAli RahimianMichael Q. SlamaKihak GwonAlan M. Gonzalez-SuarezJadon WolfHarihara BaskaranCaden D. DuffyGulnaz StybayevaQuinn P. PetersonAlexander RevzinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pouria Fattahi Ali Rahimian Michael Q. Slama Kihak Gwon Alan M. Gonzalez-Suarez Jadon Wolf Harihara Baskaran Caden D. Duffy Gulnaz Stybayeva Quinn P. Peterson Alexander Revzin Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
description	Abstract Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.
format	article
author	Pouria Fattahi Ali Rahimian Michael Q. Slama Kihak Gwon Alan M. Gonzalez-Suarez Jadon Wolf Harihara Baskaran Caden D. Duffy Gulnaz Stybayeva Quinn P. Peterson Alexander Revzin
author_facet	Pouria Fattahi Ali Rahimian Michael Q. Slama Kihak Gwon Alan M. Gonzalez-Suarez Jadon Wolf Harihara Baskaran Caden D. Duffy Gulnaz Stybayeva Quinn P. Peterson Alexander Revzin
author_sort	Pouria Fattahi
title	Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
title_short	Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
title_full	Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
title_fullStr	Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
title_full_unstemmed	Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
title_sort	core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/53e0a7662aba4e918a6dcc5423f60520
work_keys_str_mv	AT pouriafattahi coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT alirahimian coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT michaelqslama coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT kihakgwon coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT alanmgonzalezsuarez coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT jadonwolf coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT hariharabaskaran coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT cadendduffy coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT gulnazstybayeva coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT quinnppeterson coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor AT alexanderrevzin coreshellhydrogelmicrocapsulesenableformationofhumanpluripotentstemcellspheroidsandtheircultivationinastirredbioreactor
_version_	1718393029376606208

Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

Ejemplares similares