Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in Drosophila

Heme oxygenase (HO) is one of the cytoprotective enzymes that can mitigate the effects of oxidative stress. Here, we found that the ho mRNA level oscillates in the brain of Drosophila melanogaster with two minima at the beginning of the day and night. This rhyth...

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Autores principales:	Terence Al L. Abaquita, Milena Damulewicz, Debarati Bhattacharya, Elżbieta Pyza
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	reactive oxygen species (ROS) antioxidant circadian clock neuroprotection aging paraquat Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/53e6474e3cae47c185855d87852e66b2
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spelling	oai:doaj.org-article:53e6474e3cae47c185855d87852e66b22021-11-25T16:27:04ZRegulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>10.3390/antiox101117162076-3921https://doaj.org/article/53e6474e3cae47c185855d87852e66b22021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1716https://doaj.org/toc/2076-3921Heme oxygenase (HO) is one of the cytoprotective enzymes that can mitigate the effects of oxidative stress. Here, we found that the <i>ho</i> mRNA level oscillates in the brain of <i>Drosophila melanogaster</i> with two minima at the beginning of the day and night. This rhythm was partly masked by light as its pattern changed in constant darkness (DD). It followed a similar trend in the clock mutant <i>per<sup>01</sup></i> under light/dark regime (LD12:12); however, differences between time points were not statistically significant. In older flies (20 days old), the rhythm was vanished; however, 15 days of curcumin feeding restored this rhythm with an elevated <i>ho</i> mRNA level at all time points studied. In addition, flies exposed to paraquat had higher <i>ho</i> expression in the brain, but only at a specific time of the day which can be a protective response of the brain against stress. These findings suggest that the expression of <i>ho</i> in the fly’s brain is regulated by the circadian clock, light, age, exposure to stress, and the presence of exogenous antioxidants. We also found that HO cross-talks with apoptosis and autophagy under different conditions. Induction of neuronal <i>ho</i> was accompanied by increased transcription of apoptosis and autophagy-related genes. However, this trend changed after exposure to curcumin and paraquat. Our results suggest that HO is involved in the control of apoptotic and autophagic key processes protecting the brain against oxidative damage.Terence Al L. AbaquitaMilena DamulewiczDebarati BhattacharyaElżbieta PyzaMDPI AGarticlereactive oxygen species (ROS)antioxidantcircadian clockneuroprotectionagingparaquatTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1716, p 1716 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	reactive oxygen species (ROS) antioxidant circadian clock neuroprotection aging paraquat Therapeutics. Pharmacology RM1-950
spellingShingle	reactive oxygen species (ROS) antioxidant circadian clock neuroprotection aging paraquat Therapeutics. Pharmacology RM1-950 Terence Al L. Abaquita Milena Damulewicz Debarati Bhattacharya Elżbieta Pyza Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>
description	Heme oxygenase (HO) is one of the cytoprotective enzymes that can mitigate the effects of oxidative stress. Here, we found that the <i>ho</i> mRNA level oscillates in the brain of <i>Drosophila melanogaster</i> with two minima at the beginning of the day and night. This rhythm was partly masked by light as its pattern changed in constant darkness (DD). It followed a similar trend in the clock mutant <i>per<sup>01</sup></i> under light/dark regime (LD12:12); however, differences between time points were not statistically significant. In older flies (20 days old), the rhythm was vanished; however, 15 days of curcumin feeding restored this rhythm with an elevated <i>ho</i> mRNA level at all time points studied. In addition, flies exposed to paraquat had higher <i>ho</i> expression in the brain, but only at a specific time of the day which can be a protective response of the brain against stress. These findings suggest that the expression of <i>ho</i> in the fly’s brain is regulated by the circadian clock, light, age, exposure to stress, and the presence of exogenous antioxidants. We also found that HO cross-talks with apoptosis and autophagy under different conditions. Induction of neuronal <i>ho</i> was accompanied by increased transcription of apoptosis and autophagy-related genes. However, this trend changed after exposure to curcumin and paraquat. Our results suggest that HO is involved in the control of apoptotic and autophagic key processes protecting the brain against oxidative damage.
format	article
author	Terence Al L. Abaquita Milena Damulewicz Debarati Bhattacharya Elżbieta Pyza
author_facet	Terence Al L. Abaquita Milena Damulewicz Debarati Bhattacharya Elżbieta Pyza
author_sort	Terence Al L. Abaquita
title	Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>
title_short	Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>
title_full	Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>
title_fullStr	Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>
title_full_unstemmed	Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>
title_sort	regulation of heme oxygenase and its cross-talks with apoptosis and autophagy under different conditions in <i>drosophila</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/53e6474e3cae47c185855d87852e66b2
work_keys_str_mv	AT terenceallabaquita regulationofhemeoxygenaseanditscrosstalkswithapoptosisandautophagyunderdifferentconditionsinidrosophilai AT milenadamulewicz regulationofhemeoxygenaseanditscrosstalkswithapoptosisandautophagyunderdifferentconditionsinidrosophilai AT debaratibhattacharya regulationofhemeoxygenaseanditscrosstalkswithapoptosisandautophagyunderdifferentconditionsinidrosophilai AT elzbietapyza regulationofhemeoxygenaseanditscrosstalkswithapoptosisandautophagyunderdifferentconditionsinidrosophilai
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Regulation of Heme Oxygenase and Its Cross-Talks with Apoptosis and Autophagy under Different Conditions in <i>Drosophila</i>

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