Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD

Han Zhang,1 Qinghui Niu,1 Kun Liang,2 Xuesen Li,3 Jing Jiang,2 Cheng Bian2 1Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 2Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, Shandong,...

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Autores principales:	Zhang H, Niu Q, Liang K, Li X, Jiang J, Bian C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	pvt1 mir-20a-5p nafld insulin sensitivity lipid metabolism Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/53f47f04061f4076806950bced3b6aa5
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spelling	oai:doaj.org-article:53f47f04061f4076806950bced3b6aa52021-11-23T18:43:01ZEffect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD1178-7007https://doaj.org/article/53f47f04061f4076806950bced3b6aa52021-11-01T00:00:00Zhttps://www.dovepress.com/effect-of-lncpvt1mir-20a-5p-on-lipid-metabolism-and-insulin-resistance-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Han Zhang,1 Qinghui Niu,1 Kun Liang,2 Xuesen Li,3 Jing Jiang,2 Cheng Bian2 1Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 2Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 3Department of Spine Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of ChinaCorrespondence: Qinghui NiuDepartment of Liver Center, The Affiliated Hospital of Qingdao University, No. 59 Haier Road, Qingdao, Shandong, 266100, People’s Republic of ChinaTel +86-0532-82915998Email qinghuiniu@163.comPurpose: Nonalcoholic fatty liver disease (NAFLD) is closely related to lipid metabolism and insulin resistance. The current research mainly attempted to verify the clinical value of LncRNA plasmacytoma variant translocation 1 (PVT1), and whether microRNA regulates lipid metabolism and insulin resistance to participate in NAFLD.Patients and Methods: 81 patients with NAFLD and 78 healthy individuals were enrolled in this study. In addition, C57BL/6 mice were fed a high-fat diet to establish NAFLD model in vivo. Serum PVT1 and miR-20a-5p expression in NAFLD patients and mice were assessed by RT-qPCR. ROC curves determine the diagnostic value of PVT1 and miR-20a-5p. NAFLD mice were subjected to IPGTT to detect changes in insulin sensitivity, and the common indicators of lipid metabolism and insulin resistance were also evaluated. Dual-luciferase reporter assay verified the regulation mechanism of PVT1 and miR-20a-5p.Results: PVT1 was upregulated in NAFLD patients and mice, while miR-20a-5p was decreased. Their expression trends were similar in patients with HOMA-IR ≥ 2.5. What’s more, miR-20a-5p, FBG, ALT, and HOMA-IR were independently correlated with PVT1. And PVT1 and miR-20a-5p show high clinical diagnostic value. Bodyweight, insulin sensitivity, lipid metabolism inductors were increased in NAFLD mice, but these increases were attenuated by PVT1 elimination. Finally, miR-20a-5p might function as the possible miRNA target of PVT1 via the binding sites at 3ʹ-UTR and negatively regulated by it.Conclusion: PVT1 and miR-20a-5p are potential clinical biomarkers of NAFLD, and PVT1 promotes the occurrence of NAFLD by regulating insulin sensitivity and lipid metabolism, which may be achieved by targeting miR-20a-5p.Keywords: PVT1, miR-20a-5p, NAFLD, insulin sensitivity, lipid metabolismZhang HNiu QLiang KLi XJiang JBian CDove Medical Pressarticlepvt1mir-20a-5pnafldinsulin sensitivitylipid metabolismSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 4599-4608 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pvt1 mir-20a-5p nafld insulin sensitivity lipid metabolism Specialties of internal medicine RC581-951
spellingShingle	pvt1 mir-20a-5p nafld insulin sensitivity lipid metabolism Specialties of internal medicine RC581-951 Zhang H Niu Q Liang K Li X Jiang J Bian C Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD
description	Han Zhang,1 Qinghui Niu,1 Kun Liang,2 Xuesen Li,3 Jing Jiang,2 Cheng Bian2 1Department of Liver Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 2Department of Infectious Diseases, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 3Department of Spine Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of ChinaCorrespondence: Qinghui NiuDepartment of Liver Center, The Affiliated Hospital of Qingdao University, No. 59 Haier Road, Qingdao, Shandong, 266100, People’s Republic of ChinaTel +86-0532-82915998Email qinghuiniu@163.comPurpose: Nonalcoholic fatty liver disease (NAFLD) is closely related to lipid metabolism and insulin resistance. The current research mainly attempted to verify the clinical value of LncRNA plasmacytoma variant translocation 1 (PVT1), and whether microRNA regulates lipid metabolism and insulin resistance to participate in NAFLD.Patients and Methods: 81 patients with NAFLD and 78 healthy individuals were enrolled in this study. In addition, C57BL/6 mice were fed a high-fat diet to establish NAFLD model in vivo. Serum PVT1 and miR-20a-5p expression in NAFLD patients and mice were assessed by RT-qPCR. ROC curves determine the diagnostic value of PVT1 and miR-20a-5p. NAFLD mice were subjected to IPGTT to detect changes in insulin sensitivity, and the common indicators of lipid metabolism and insulin resistance were also evaluated. Dual-luciferase reporter assay verified the regulation mechanism of PVT1 and miR-20a-5p.Results: PVT1 was upregulated in NAFLD patients and mice, while miR-20a-5p was decreased. Their expression trends were similar in patients with HOMA-IR ≥ 2.5. What’s more, miR-20a-5p, FBG, ALT, and HOMA-IR were independently correlated with PVT1. And PVT1 and miR-20a-5p show high clinical diagnostic value. Bodyweight, insulin sensitivity, lipid metabolism inductors were increased in NAFLD mice, but these increases were attenuated by PVT1 elimination. Finally, miR-20a-5p might function as the possible miRNA target of PVT1 via the binding sites at 3ʹ-UTR and negatively regulated by it.Conclusion: PVT1 and miR-20a-5p are potential clinical biomarkers of NAFLD, and PVT1 promotes the occurrence of NAFLD by regulating insulin sensitivity and lipid metabolism, which may be achieved by targeting miR-20a-5p.Keywords: PVT1, miR-20a-5p, NAFLD, insulin sensitivity, lipid metabolism
format	article
author	Zhang H Niu Q Liang K Li X Jiang J Bian C
author_facet	Zhang H Niu Q Liang K Li X Jiang J Bian C
author_sort	Zhang H
title	Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD
title_short	Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD
title_full	Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD
title_fullStr	Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD
title_full_unstemmed	Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD
title_sort	effect of lncpvt1/mir-20a-5p on lipid metabolism and insulin resistance in nafld
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/53f47f04061f4076806950bced3b6aa5
work_keys_str_mv	AT zhangh effectoflncpvt1mir20a5ponlipidmetabolismandinsulinresistanceinnafld AT niuq effectoflncpvt1mir20a5ponlipidmetabolismandinsulinresistanceinnafld AT liangk effectoflncpvt1mir20a5ponlipidmetabolismandinsulinresistanceinnafld AT lix effectoflncpvt1mir20a5ponlipidmetabolismandinsulinresistanceinnafld AT jiangj effectoflncpvt1mir20a5ponlipidmetabolismandinsulinresistanceinnafld AT bianc effectoflncpvt1mir20a5ponlipidmetabolismandinsulinresistanceinnafld
_version_	1718416154960068608

Effect of LncPVT1/miR-20a-5p on Lipid Metabolism and Insulin Resistance in NAFLD

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