The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis

Background: The loop-mediated isothermal amplification (LAMP) method is frequently used for identifying many microorganisms. The present review aimed to evaluate the sensitivity and specificity of LAMP method for detection of food-borne bacteria and to compare these features with those of polymeras...

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Autores principales:	Yasaman Sadeghi, Pegah Kananizadeh, Solmaz Ohadian Moghadam, Ahad Alizadeh, Mohammad Reza Pourmand, Neda Mohammadi, Davoud Afshar, Reza Ranjbar
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	Food-Borne Pathogen Specificity Sensitivity Loop-mediated isothermal amplification (LAMP) Polymerase chain reaction Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/53fcf02d033749e4ad8f91097e8fb857
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spelling	oai:doaj.org-article:53fcf02d033749e4ad8f91097e8fb8572021-12-02T19:21:44ZThe Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis2251-60852251-6093https://doaj.org/article/53fcf02d033749e4ad8f91097e8fb8572021-10-01T00:00:00Zhttps://ijph.tums.ac.ir/index.php/ijph/article/view/14960https://doaj.org/toc/2251-6085https://doaj.org/toc/2251-6093 Background: The loop-mediated isothermal amplification (LAMP) method is frequently used for identifying many microorganisms. The present review aimed to evaluate the sensitivity and specificity of LAMP method for detection of food-borne bacteria and to compare these features with those of polymerase chain reaction (PCR), as an alternative molecular diagnostic procedure, and with cultivation method, as the gold standard method. Methods: The literature was searched in electronic databases (PubMed, Scopus, Web of Science, and EMBASE) for recruiting publications within Jan 2000 to Jul 2021. We used the combinations of keywords including foodborne disease, LAMP, PCR, Loop-mediated isothermal amplification, and polymerase chain reaction. Meta-analysis was used to adjust the correlation and heterogeneity between the studies. The efficiency of the methods was presented by negative likelihood ratio, positive likelihood ratio, sensitivity, specificity, and odds ratio using forest plots. A P-value less than 0.05 was considered as statistical significance cut off. The confidence intervals were presented at the 95% interval. Results: Overall, 23 relevant studies were analyzed. The sensitivities of LAMP and PCR methods were estimated to be 96.6% (95% CI: 95.0-97.7) and 95.6% (95%CI: 91.5-97.8), respectively. The specificities of LAMP and PCR were also estimated to be 97.6% (95%CI: 92.6-99.3) and 98.7% (95%CI: 96.5-99.5), respectively. Conclusion: The specificities of LAMP and PCR assays were determined by comparing their results with cultivation method as the gold standard. Overall, the specificity of both PCR and LAMP methods was low for detection of fastidious bacteria. Nevertheless, LAMP and PCR methods have acceptable specificities and sensitivities, and their application in clinical practice necessitates more studies. Yasaman SadeghiPegah KananizadehSolmaz Ohadian MoghadamAhad AlizadehMohammad Reza PourmandNeda MohammadiDavoud AfsharReza RanjbarTehran University of Medical SciencesarticleFood-Borne PathogenSpecificitySensitivityLoop-mediated isothermal amplification (LAMP)Polymerase chain reactionPublic aspects of medicineRA1-1270ENIranian Journal of Public Health, Vol 50, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Food-Borne Pathogen Specificity Sensitivity Loop-mediated isothermal amplification (LAMP) Polymerase chain reaction Public aspects of medicine RA1-1270
spellingShingle	Food-Borne Pathogen Specificity Sensitivity Loop-mediated isothermal amplification (LAMP) Polymerase chain reaction Public aspects of medicine RA1-1270 Yasaman Sadeghi Pegah Kananizadeh Solmaz Ohadian Moghadam Ahad Alizadeh Mohammad Reza Pourmand Neda Mohammadi Davoud Afshar Reza Ranjbar The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis
description	Background: The loop-mediated isothermal amplification (LAMP) method is frequently used for identifying many microorganisms. The present review aimed to evaluate the sensitivity and specificity of LAMP method for detection of food-borne bacteria and to compare these features with those of polymerase chain reaction (PCR), as an alternative molecular diagnostic procedure, and with cultivation method, as the gold standard method. Methods: The literature was searched in electronic databases (PubMed, Scopus, Web of Science, and EMBASE) for recruiting publications within Jan 2000 to Jul 2021. We used the combinations of keywords including foodborne disease, LAMP, PCR, Loop-mediated isothermal amplification, and polymerase chain reaction. Meta-analysis was used to adjust the correlation and heterogeneity between the studies. The efficiency of the methods was presented by negative likelihood ratio, positive likelihood ratio, sensitivity, specificity, and odds ratio using forest plots. A P-value less than 0.05 was considered as statistical significance cut off. The confidence intervals were presented at the 95% interval. Results: Overall, 23 relevant studies were analyzed. The sensitivities of LAMP and PCR methods were estimated to be 96.6% (95% CI: 95.0-97.7) and 95.6% (95%CI: 91.5-97.8), respectively. The specificities of LAMP and PCR were also estimated to be 97.6% (95%CI: 92.6-99.3) and 98.7% (95%CI: 96.5-99.5), respectively. Conclusion: The specificities of LAMP and PCR assays were determined by comparing their results with cultivation method as the gold standard. Overall, the specificity of both PCR and LAMP methods was low for detection of fastidious bacteria. Nevertheless, LAMP and PCR methods have acceptable specificities and sensitivities, and their application in clinical practice necessitates more studies.
format	article
author	Yasaman Sadeghi Pegah Kananizadeh Solmaz Ohadian Moghadam Ahad Alizadeh Mohammad Reza Pourmand Neda Mohammadi Davoud Afshar Reza Ranjbar
author_facet	Yasaman Sadeghi Pegah Kananizadeh Solmaz Ohadian Moghadam Ahad Alizadeh Mohammad Reza Pourmand Neda Mohammadi Davoud Afshar Reza Ranjbar
author_sort	Yasaman Sadeghi
title	The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis
title_short	The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis
title_full	The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis
title_fullStr	The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis
title_full_unstemmed	The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis
title_sort	sensitivity and specificity of loop-mediated isothermal amplification and pcr methods in detection of foodborne microorganisms: a systematic review and meta-analysis
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/53fcf02d033749e4ad8f91097e8fb857
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The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis

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