Human cathelicidin production by the cervix.

hCAP18/LL-37 is the sole human cathelicidin; a family of host defence peptides with key roles in innate host defence. hCAP18/LL-37 is expressed primarily by neutrophils and epithelial cells, but its production and function in the lower genital tract is largely uncharacterised. Despite the significan...

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Autores principales:	Lorraine Frew, Sofia Makieva, Andrew T M McKinlay, Brian J McHugh, Ann Doust, Jane E Norman, Donald J Davidson, Sarah J Stock
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5400e4b01f8042dea814e303837d241e
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spelling	oai:doaj.org-article:5400e4b01f8042dea814e303837d241e2021-11-25T06:06:09ZHuman cathelicidin production by the cervix.1932-620310.1371/journal.pone.0103434https://doaj.org/article/5400e4b01f8042dea814e303837d241e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25089904/?tool=EBIhttps://doaj.org/toc/1932-6203hCAP18/LL-37 is the sole human cathelicidin; a family of host defence peptides with key roles in innate host defence. hCAP18/LL-37 is expressed primarily by neutrophils and epithelial cells, but its production and function in the lower genital tract is largely uncharacterised. Despite the significant roles for cathelicidin in multiple organs and inflammatory processes, its impact on infections that could compromise fertility and pregnancy is unknown. The aim of this study was to investigate cathelicidin production, regulation and function in the cervix. hCAP18/LL-37 was found to be present in cervicovaginal secretions collected from women in the first trimester of pregnancy and to be expressed at significantly higher levels in samples from women with alterations in vaginal bacterial flora characteristic of bacterial vaginosis. In endocervical epithelial cell lines, expression of the gene encoding hCAP18/LL-37 (CAMP) was not affected by TLR agonists, but was found to be up-regulated by both 1, 25 hydroxyvitamin D3 and 25 hydroxyvitamin D3. However, no association was found between serum levels of vitamin D and hCAP18/LL-37 concentrations in cervicovaginal secretions (n = 116). Exposure to synthetic LL-37 had a pro-inflammatory effect on endocervical epithelial cell lines, increasing secretion of inflammatory cytokine IL-8. Together these data demonstrate inducible expression of hCAP18/LL-37 in the female lower reproductive tract in vivo and suggest the capacity for this peptide to modulate host defence to infection in this system. Further investigation will elucidate the effects of hCAP18/LL-37 on the physiology and pathophysiology of labour, and may lead to strategies for the prevention of infection-associated preterm birth.Lorraine FrewSofia MakievaAndrew T M McKinlayBrian J McHughAnn DoustJane E NormanDonald J DavidsonSarah J StockPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103434 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorraine Frew Sofia Makieva Andrew T M McKinlay Brian J McHugh Ann Doust Jane E Norman Donald J Davidson Sarah J Stock Human cathelicidin production by the cervix.
description	hCAP18/LL-37 is the sole human cathelicidin; a family of host defence peptides with key roles in innate host defence. hCAP18/LL-37 is expressed primarily by neutrophils and epithelial cells, but its production and function in the lower genital tract is largely uncharacterised. Despite the significant roles for cathelicidin in multiple organs and inflammatory processes, its impact on infections that could compromise fertility and pregnancy is unknown. The aim of this study was to investigate cathelicidin production, regulation and function in the cervix. hCAP18/LL-37 was found to be present in cervicovaginal secretions collected from women in the first trimester of pregnancy and to be expressed at significantly higher levels in samples from women with alterations in vaginal bacterial flora characteristic of bacterial vaginosis. In endocervical epithelial cell lines, expression of the gene encoding hCAP18/LL-37 (CAMP) was not affected by TLR agonists, but was found to be up-regulated by both 1, 25 hydroxyvitamin D3 and 25 hydroxyvitamin D3. However, no association was found between serum levels of vitamin D and hCAP18/LL-37 concentrations in cervicovaginal secretions (n = 116). Exposure to synthetic LL-37 had a pro-inflammatory effect on endocervical epithelial cell lines, increasing secretion of inflammatory cytokine IL-8. Together these data demonstrate inducible expression of hCAP18/LL-37 in the female lower reproductive tract in vivo and suggest the capacity for this peptide to modulate host defence to infection in this system. Further investigation will elucidate the effects of hCAP18/LL-37 on the physiology and pathophysiology of labour, and may lead to strategies for the prevention of infection-associated preterm birth.
format	article
author	Lorraine Frew Sofia Makieva Andrew T M McKinlay Brian J McHugh Ann Doust Jane E Norman Donald J Davidson Sarah J Stock
author_facet	Lorraine Frew Sofia Makieva Andrew T M McKinlay Brian J McHugh Ann Doust Jane E Norman Donald J Davidson Sarah J Stock
author_sort	Lorraine Frew
title	Human cathelicidin production by the cervix.
title_short	Human cathelicidin production by the cervix.
title_full	Human cathelicidin production by the cervix.
title_fullStr	Human cathelicidin production by the cervix.
title_full_unstemmed	Human cathelicidin production by the cervix.
title_sort	human cathelicidin production by the cervix.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5400e4b01f8042dea814e303837d241e
work_keys_str_mv	AT lorrainefrew humancathelicidinproductionbythecervix AT sofiamakieva humancathelicidinproductionbythecervix AT andrewtmmckinlay humancathelicidinproductionbythecervix AT brianjmchugh humancathelicidinproductionbythecervix AT anndoust humancathelicidinproductionbythecervix AT janeenorman humancathelicidinproductionbythecervix AT donaldjdavidson humancathelicidinproductionbythecervix AT sarahjstock humancathelicidinproductionbythecervix
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Human cathelicidin production by the cervix.

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