Low frequency of HPV positivity in breast tumors among patients from south-central Poland

Abstract Background Some studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland. Materials and methods The study was performed based...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Beata Biesaga, Anna Janecka-Widła, Marta Kołodziej-Rzepa, Anna Mucha-Małecka, Dorota Słonina, Marek Ziobro, Joanna Wysocka, Agnieszka Adamczyk, Kaja Majchrzyk, Joanna Niemiec, Aleksandra Ambicka, Aleksandra Grela-Wojewoda, Agnieszka Harazin-Lechowska
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Breast cancers HPV infection South-central Poland Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/540d14998cbf45ac9d81e4dd17efb084
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:540d14998cbf45ac9d81e4dd17efb084
record_format	dspace
spelling	oai:doaj.org-article:540d14998cbf45ac9d81e4dd17efb0842021-11-28T12:11:21ZLow frequency of HPV positivity in breast tumors among patients from south-central Poland10.1186/s13027-021-00405-z1750-9378https://doaj.org/article/540d14998cbf45ac9d81e4dd17efb0842021-11-01T00:00:00Zhttps://doi.org/10.1186/s13027-021-00405-zhttps://doaj.org/toc/1750-9378Abstract Background Some studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland. Materials and methods The study was performed based on archival paraffin embebbed and formalin fixed blocks in the group of 383 patients with breast cancer. HPV prevalence and its genotype were assessed, respectively by: nested PCR (with two groups of primers: PGMY09/PGMY11 and GP5+/GP6+), quantitative PCR (qPCR). Tumors were classified as HPV positive in case of at least one positive result in nested PCR and positive results in genotyping procedure. For all HPV positive tissues P16 immunostaining was applied in order to confirm active viral infection. Results In the group of 383 breast cancers, HPV positivity was found in 17 samples (4.4%) in nested PCR. All these samples were subjected to HPV genotyping. This analysis revealed presence of HPV type 16 into two tumors (0.5%). In these two cancers, P16 overexpression was reported. Conclusion In breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases.Beata BiesagaAnna Janecka-WidłaMarta Kołodziej-RzepaAnna Mucha-MałeckaDorota SłoninaMarek ZiobroJoanna WysockaAgnieszka AdamczykKaja MajchrzykJoanna NiemiecAleksandra AmbickaAleksandra Grela-WojewodaAgnieszka Harazin-LechowskaBMCarticleBreast cancersHPV infectionSouth-central PolandNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282Infectious and parasitic diseasesRC109-216ENInfectious Agents and Cancer, Vol 16, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Breast cancers HPV infection South-central Poland Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Infectious and parasitic diseases RC109-216
spellingShingle	Breast cancers HPV infection South-central Poland Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Infectious and parasitic diseases RC109-216 Beata Biesaga Anna Janecka-Widła Marta Kołodziej-Rzepa Anna Mucha-Małecka Dorota Słonina Marek Ziobro Joanna Wysocka Agnieszka Adamczyk Kaja Majchrzyk Joanna Niemiec Aleksandra Ambicka Aleksandra Grela-Wojewoda Agnieszka Harazin-Lechowska Low frequency of HPV positivity in breast tumors among patients from south-central Poland
description	Abstract Background Some studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland. Materials and methods The study was performed based on archival paraffin embebbed and formalin fixed blocks in the group of 383 patients with breast cancer. HPV prevalence and its genotype were assessed, respectively by: nested PCR (with two groups of primers: PGMY09/PGMY11 and GP5+/GP6+), quantitative PCR (qPCR). Tumors were classified as HPV positive in case of at least one positive result in nested PCR and positive results in genotyping procedure. For all HPV positive tissues P16 immunostaining was applied in order to confirm active viral infection. Results In the group of 383 breast cancers, HPV positivity was found in 17 samples (4.4%) in nested PCR. All these samples were subjected to HPV genotyping. This analysis revealed presence of HPV type 16 into two tumors (0.5%). In these two cancers, P16 overexpression was reported. Conclusion In breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases.
format	article
author	Beata Biesaga Anna Janecka-Widła Marta Kołodziej-Rzepa Anna Mucha-Małecka Dorota Słonina Marek Ziobro Joanna Wysocka Agnieszka Adamczyk Kaja Majchrzyk Joanna Niemiec Aleksandra Ambicka Aleksandra Grela-Wojewoda Agnieszka Harazin-Lechowska
author_facet	Beata Biesaga Anna Janecka-Widła Marta Kołodziej-Rzepa Anna Mucha-Małecka Dorota Słonina Marek Ziobro Joanna Wysocka Agnieszka Adamczyk Kaja Majchrzyk Joanna Niemiec Aleksandra Ambicka Aleksandra Grela-Wojewoda Agnieszka Harazin-Lechowska
author_sort	Beata Biesaga
title	Low frequency of HPV positivity in breast tumors among patients from south-central Poland
title_short	Low frequency of HPV positivity in breast tumors among patients from south-central Poland
title_full	Low frequency of HPV positivity in breast tumors among patients from south-central Poland
title_fullStr	Low frequency of HPV positivity in breast tumors among patients from south-central Poland
title_full_unstemmed	Low frequency of HPV positivity in breast tumors among patients from south-central Poland
title_sort	low frequency of hpv positivity in breast tumors among patients from south-central poland
publisher	BMC
publishDate	2021
url	https://doaj.org/article/540d14998cbf45ac9d81e4dd17efb084
work_keys_str_mv	AT beatabiesaga lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT annajaneckawidła lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT martakołodziejrzepa lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT annamuchamałecka lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT dorotasłonina lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT marekziobro lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT joannawysocka lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT agnieszkaadamczyk lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT kajamajchrzyk lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT joannaniemiec lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT aleksandraambicka lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT aleksandragrelawojewoda lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland AT agnieszkaharazinlechowska lowfrequencyofhpvpositivityinbreasttumorsamongpatientsfromsouthcentralpoland
_version_	1718408139994300416

Low frequency of HPV positivity in breast tumors among patients from south-central Poland

Ejemplares similares