Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans

Abstract Arsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize a...

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Autores principales:	Hyeon-Woo Koh, Moonsuk Hur, Myung-Suk Kang, Youn-Bong Ku, Rohit Ghai, Soo-Je Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/540f17052803423b8296db701466ece3
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spelling	oai:doaj.org-article:540f17052803423b8296db701466ece32021-12-02T15:06:12ZPhysiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans10.1038/s41598-017-15164-42045-2322https://doaj.org/article/540f17052803423b8296db701466ece32017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15164-4https://doaj.org/toc/2045-2322Abstract Arsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize arsenite, and investigated its physiological and genomic traits. Its cells were rod-shaped and Gram-negative, and formed small beige-pigmented colonies. 16S rRNA-based phylogenetic analysis indicated that the strain belongs to the genus Herminiimonas and is closely related to Herminiimonas glaciei UMB49T (98.7% of 16S rRNA gene sequence similarity), Herminiimonas arsenicoxydans ULPAs1T (98.4%), and Herminiimonas saxobsidens NS11T (98.4%). Under chemolithoheterotrophic conditions, the strain utilized some organic acids and amino acids as carbon and/or nitrogen sources but not electron sources. Further, the strain grew as a sulfur oxidizer in a complex medium (trypticase soy agar). Unexpectedly, most carbohydrates failed to support its growth as sole carbon sources. Genome sequencing supported these observations, and very few ABC transporters capable of oligo/monosaccharide uptake were identified in the AS8 genome. The genome harbored genes required for the colonization, flagella biosynthesis, urea degradation, and heavy metal and antibiotic resistance. Based on these polyphasic and genomic analyses, we propose that the strain AS8 be named Herminiimonas arsenitoxidans.Hyeon-Woo KohMoonsuk HurMyung-Suk KangYoun-Bong KuRohit GhaiSoo-Je ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyeon-Woo Koh Moonsuk Hur Myung-Suk Kang Youn-Bong Ku Rohit Ghai Soo-Je Park Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
description	Abstract Arsenic, a representative toxic metalloid, is responsible for serious global health problems. Most organisms possess arsenic resistance strategies to mitigate this toxicity. Here, we reported a microorganism, strain AS8, from heavy metal/metalloid-contaminated soil that is able to oxidize arsenite, and investigated its physiological and genomic traits. Its cells were rod-shaped and Gram-negative, and formed small beige-pigmented colonies. 16S rRNA-based phylogenetic analysis indicated that the strain belongs to the genus Herminiimonas and is closely related to Herminiimonas glaciei UMB49T (98.7% of 16S rRNA gene sequence similarity), Herminiimonas arsenicoxydans ULPAs1T (98.4%), and Herminiimonas saxobsidens NS11T (98.4%). Under chemolithoheterotrophic conditions, the strain utilized some organic acids and amino acids as carbon and/or nitrogen sources but not electron sources. Further, the strain grew as a sulfur oxidizer in a complex medium (trypticase soy agar). Unexpectedly, most carbohydrates failed to support its growth as sole carbon sources. Genome sequencing supported these observations, and very few ABC transporters capable of oligo/monosaccharide uptake were identified in the AS8 genome. The genome harbored genes required for the colonization, flagella biosynthesis, urea degradation, and heavy metal and antibiotic resistance. Based on these polyphasic and genomic analyses, we propose that the strain AS8 be named Herminiimonas arsenitoxidans.
format	article
author	Hyeon-Woo Koh Moonsuk Hur Myung-Suk Kang Youn-Bong Ku Rohit Ghai Soo-Je Park
author_facet	Hyeon-Woo Koh Moonsuk Hur Myung-Suk Kang Youn-Bong Ku Rohit Ghai Soo-Je Park
author_sort	Hyeon-Woo Koh
title	Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
title_short	Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
title_full	Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
title_fullStr	Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
title_full_unstemmed	Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans
title_sort	physiological and genomic insights into the lifestyle of arsenite-oxidizing herminiimonas arsenitoxidans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/540f17052803423b8296db701466ece3
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Physiological and genomic insights into the lifestyle of arsenite-oxidizing Herminiimonas arsenitoxidans

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