Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups
Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton Х-305 and Triton Х-405. Total hydrolase activity of ur...
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Scientific Сentre for Family Health and Human Reproduction Problems
2016
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oai:doaj.org-article:5418e6051a60469db4230eb48193ce732021-11-23T06:14:34ZZymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups2541-94202587-959610.12737/20616https://doaj.org/article/5418e6051a60469db4230eb48193ce732016-04-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/171https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton Х-305 and Triton Х-405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton Х-405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons - preparations of urea extracts from the toxigenic strains (p < 0,05).S. N. KozlovV. B. NikolaevE. Y. MarkovL. Y. UrbanovichS. K. MitkeevaScientific Сentre for Family Health and Human Reproduction Problemsarticlevibrio choleraezymographic analysissubcellular fractionlipolytical enzymevibrio choleraesubstrate electrophoresisScienceQRUActa Biomedica Scientifica, Vol 1, Iss 2, Pp 55-59 (2016) |
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DOAJ |
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RU |
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vibrio cholerae zymographic analysis subcellular fraction lipolytical enzyme vibrio cholerae substrate electrophoresis Science Q |
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vibrio cholerae zymographic analysis subcellular fraction lipolytical enzyme vibrio cholerae substrate electrophoresis Science Q S. N. Kozlov V. B. Nikolaev E. Y. Markov L. Y. Urbanovich S. K. Mitkeeva Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups |
| description |
Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton Х-305 and Triton Х-405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton Х-405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons - preparations of urea extracts from the toxigenic strains (p < 0,05). |
| format |
article |
| author |
S. N. Kozlov V. B. Nikolaev E. Y. Markov L. Y. Urbanovich S. K. Mitkeeva |
| author_facet |
S. N. Kozlov V. B. Nikolaev E. Y. Markov L. Y. Urbanovich S. K. Mitkeeva |
| author_sort |
S. N. Kozlov |
| title |
Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups |
| title_short |
Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups |
| title_full |
Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups |
| title_fullStr |
Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups |
| title_full_unstemmed |
Zymographic analysis of lipolytical enzymes of subcellular Vibrio cholerae fractions of O1 and O139 serogroups |
| title_sort |
zymographic analysis of lipolytical enzymes of subcellular vibrio cholerae fractions of o1 and o139 serogroups |
| publisher |
Scientific Сentre for Family Health and Human Reproduction Problems |
| publishDate |
2016 |
| url |
https://doaj.org/article/5418e6051a60469db4230eb48193ce73 |
| work_keys_str_mv |
AT snkozlov zymographicanalysisoflipolyticalenzymesofsubcellularvibriocholeraefractionsofo1ando139serogroups AT vbnikolaev zymographicanalysisoflipolyticalenzymesofsubcellularvibriocholeraefractionsofo1ando139serogroups AT eymarkov zymographicanalysisoflipolyticalenzymesofsubcellularvibriocholeraefractionsofo1ando139serogroups AT lyurbanovich zymographicanalysisoflipolyticalenzymesofsubcellularvibriocholeraefractionsofo1ando139serogroups AT skmitkeeva zymographicanalysisoflipolyticalenzymesofsubcellularvibriocholeraefractionsofo1ando139serogroups |
| _version_ |
1718417069816414208 |