Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats

Introduction. Knowledge about the effects of medications, vitamins, and various supplements on orthodontic tooth movement (OTM) is imperative for orthodontists. This study aimed to assess the effect of methotrexate (MTX) injection on OTM in rats. Materials and Methods. Twenty-eight male Wistar rats...

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Autores principales:	Amin Golshah, Khaled Omidi, Nafiseh Nikkerdar, Hedaiat Moradpoor, Fatemeh Ghorbani
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/5419996f0d554c68aec6673184269ce6
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spelling	oai:doaj.org-article:5419996f0d554c68aec6673184269ce62021-11-22T01:11:32ZEffect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats1687-873610.1155/2021/8451522https://doaj.org/article/5419996f0d554c68aec6673184269ce62021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8451522https://doaj.org/toc/1687-8736Introduction. Knowledge about the effects of medications, vitamins, and various supplements on orthodontic tooth movement (OTM) is imperative for orthodontists. This study aimed to assess the effect of methotrexate (MTX) injection on OTM in rats. Materials and Methods. Twenty-eight male Wistar rats were randomized into four groups (n = 7). The first molar and central incisor were connected using a nickel-titanium (NiTi) coil spring with a 50 g load in each rat. The two experimental groups received 0.75 mg/kg and 1.5 mg/kg MTX, respectively, intraperitoneally for 21 days. The negative control group did not receive any injection and did not undergo orthodontic treatment. The positive control group underwent orthodontic treatment and received 0.9% saline (NaCl) injections for 21 days. All rats were sacrificed with chloroform inhalation after 21 days; their maxilla was resected, and the mean number of Howship’s lacunae, blood vessels, osteoclasts, and resorption lacunae was counted. The reduction in bone volume (bone volume to total volume ratio (BV/TV)) at the site of the maxillary molar was quantified by microcomputed tomography (micro-CT). Results. OTM, the number of osteoclasts, and the number of blood vessels significantly increased in rats treated with MTX (P<0.05). However, the increase in the number of Howship’s lacunae and resorption lacunae was not significant (P>0.05). Lower BV/TV in the MTX groups was in agreement with the increased number of osteoclasts. Conclusion. Injection of MTX can significantly increase OTM and decrease root resorption in rats.Amin GolshahKhaled OmidiNafiseh NikkerdarHedaiat MoradpoorFatemeh GhorbaniHindawi LimitedarticleDentistryRK1-715ENInternational Journal of Dentistry, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dentistry RK1-715
spellingShingle	Dentistry RK1-715 Amin Golshah Khaled Omidi Nafiseh Nikkerdar Hedaiat Moradpoor Fatemeh Ghorbani Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats
description	Introduction. Knowledge about the effects of medications, vitamins, and various supplements on orthodontic tooth movement (OTM) is imperative for orthodontists. This study aimed to assess the effect of methotrexate (MTX) injection on OTM in rats. Materials and Methods. Twenty-eight male Wistar rats were randomized into four groups (n = 7). The first molar and central incisor were connected using a nickel-titanium (NiTi) coil spring with a 50 g load in each rat. The two experimental groups received 0.75 mg/kg and 1.5 mg/kg MTX, respectively, intraperitoneally for 21 days. The negative control group did not receive any injection and did not undergo orthodontic treatment. The positive control group underwent orthodontic treatment and received 0.9% saline (NaCl) injections for 21 days. All rats were sacrificed with chloroform inhalation after 21 days; their maxilla was resected, and the mean number of Howship’s lacunae, blood vessels, osteoclasts, and resorption lacunae was counted. The reduction in bone volume (bone volume to total volume ratio (BV/TV)) at the site of the maxillary molar was quantified by microcomputed tomography (micro-CT). Results. OTM, the number of osteoclasts, and the number of blood vessels significantly increased in rats treated with MTX (P<0.05). However, the increase in the number of Howship’s lacunae and resorption lacunae was not significant (P>0.05). Lower BV/TV in the MTX groups was in agreement with the increased number of osteoclasts. Conclusion. Injection of MTX can significantly increase OTM and decrease root resorption in rats.
format	article
author	Amin Golshah Khaled Omidi Nafiseh Nikkerdar Hedaiat Moradpoor Fatemeh Ghorbani
author_facet	Amin Golshah Khaled Omidi Nafiseh Nikkerdar Hedaiat Moradpoor Fatemeh Ghorbani
author_sort	Amin Golshah
title	Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats
title_short	Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats
title_full	Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats
title_fullStr	Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats
title_full_unstemmed	Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats
title_sort	effect of methotrexate injection on orthodontic tooth movement: an experimental study on rats
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/5419996f0d554c68aec6673184269ce6
work_keys_str_mv	AT amingolshah effectofmethotrexateinjectiononorthodontictoothmovementanexperimentalstudyonrats AT khaledomidi effectofmethotrexateinjectiononorthodontictoothmovementanexperimentalstudyonrats AT nafisehnikkerdar effectofmethotrexateinjectiononorthodontictoothmovementanexperimentalstudyonrats AT hedaiatmoradpoor effectofmethotrexateinjectiononorthodontictoothmovementanexperimentalstudyonrats AT fatemehghorbani effectofmethotrexateinjectiononorthodontictoothmovementanexperimentalstudyonrats
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Effect of Methotrexate Injection on Orthodontic Tooth Movement: An Experimental Study on Rats

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