Ultrasonic atomizer based development of pH sensor for real time analysis

Abstract Fluorescent pH biosensors have gained importance owing to their low cost utilization in real time monitoring of biological and food samples in comparison to conventional pH meters. The research reports a novel method of ultrasonic atomization for developing a fluorescent pH sensor for real-...

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Autores principales:	Gaurav Pandey, Sandeep Choudhary, Rashmi Chaudhari, Abhijeet Joshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/541cfcfebdb84d1db83f26580fb4c7fe
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spelling	oai:doaj.org-article:541cfcfebdb84d1db83f26580fb4c7fe2021-12-02T16:31:42ZUltrasonic atomizer based development of pH sensor for real time analysis10.1038/s41598-020-68005-22045-2322https://doaj.org/article/541cfcfebdb84d1db83f26580fb4c7fe2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68005-2https://doaj.org/toc/2045-2322Abstract Fluorescent pH biosensors have gained importance owing to their low cost utilization in real time monitoring of biological and food samples in comparison to conventional pH meters. The research reports a novel method of ultrasonic atomization for developing a fluorescent pH sensor for real-time analysis made of Fluorescein isothiocyanate (FITC)-dextran/FITC-dextran-Tris (2, 2′-bipyridyl) dichlororuthenium (II) hexahydrate as indicator and reference fluorophores, respectively. The process of ultrasonic atomization ensures formation of monodisperse dye immobilized alginate microspheres ensuring efficient pH sensing. The developed biosensor was tested on milk samples, which has a short life span and shows a significant fall in pH with time due to microbial spoilage. The proposed biosensor showed a linear range of pH 4–8 (R2 between 0.96–0.99 for different single/dual fluorophore biosensors) which suitably cover the pH of milk during the entire storage period and spoilage. The % recovery for predicted pH falls between 90–110% compared against standard pH meter, indicating a good accuracy of estimation and low turnaround time (10 min). Thus, real-time monitoring using fluorescent pH biosensor for milk samples may profoundly improve the economics of losses occurring in processing and storage with capability of in-package continuous quality assessment.Gaurav PandeySandeep ChoudharyRashmi ChaudhariAbhijeet JoshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gaurav Pandey Sandeep Choudhary Rashmi Chaudhari Abhijeet Joshi Ultrasonic atomizer based development of pH sensor for real time analysis
description	Abstract Fluorescent pH biosensors have gained importance owing to their low cost utilization in real time monitoring of biological and food samples in comparison to conventional pH meters. The research reports a novel method of ultrasonic atomization for developing a fluorescent pH sensor for real-time analysis made of Fluorescein isothiocyanate (FITC)-dextran/FITC-dextran-Tris (2, 2′-bipyridyl) dichlororuthenium (II) hexahydrate as indicator and reference fluorophores, respectively. The process of ultrasonic atomization ensures formation of monodisperse dye immobilized alginate microspheres ensuring efficient pH sensing. The developed biosensor was tested on milk samples, which has a short life span and shows a significant fall in pH with time due to microbial spoilage. The proposed biosensor showed a linear range of pH 4–8 (R2 between 0.96–0.99 for different single/dual fluorophore biosensors) which suitably cover the pH of milk during the entire storage period and spoilage. The % recovery for predicted pH falls between 90–110% compared against standard pH meter, indicating a good accuracy of estimation and low turnaround time (10 min). Thus, real-time monitoring using fluorescent pH biosensor for milk samples may profoundly improve the economics of losses occurring in processing and storage with capability of in-package continuous quality assessment.
format	article
author	Gaurav Pandey Sandeep Choudhary Rashmi Chaudhari Abhijeet Joshi
author_facet	Gaurav Pandey Sandeep Choudhary Rashmi Chaudhari Abhijeet Joshi
author_sort	Gaurav Pandey
title	Ultrasonic atomizer based development of pH sensor for real time analysis
title_short	Ultrasonic atomizer based development of pH sensor for real time analysis
title_full	Ultrasonic atomizer based development of pH sensor for real time analysis
title_fullStr	Ultrasonic atomizer based development of pH sensor for real time analysis
title_full_unstemmed	Ultrasonic atomizer based development of pH sensor for real time analysis
title_sort	ultrasonic atomizer based development of ph sensor for real time analysis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/541cfcfebdb84d1db83f26580fb4c7fe
work_keys_str_mv	AT gauravpandey ultrasonicatomizerbaseddevelopmentofphsensorforrealtimeanalysis AT sandeepchoudhary ultrasonicatomizerbaseddevelopmentofphsensorforrealtimeanalysis AT rashmichaudhari ultrasonicatomizerbaseddevelopmentofphsensorforrealtimeanalysis AT abhijeetjoshi ultrasonicatomizerbaseddevelopmentofphsensorforrealtimeanalysis
_version_	1718383871701024768

Ultrasonic atomizer based development of pH sensor for real time analysis

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