The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum

Alternative splicing (AS) occurs extensively in eukaryotes as an essential mechanism for regulating transcriptome complexity and diversity, but the AS landscape regulating potassium (K) use efficiency in plants is unclear. In this study, we performed high-throughput transcriptome sequencing of roots...

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Autores principales:	Bing He, Lin Meng, Lina Tang, Weicong Qi, Fengqin Hu, Yuanda Lv, Wenjing Song
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	potassium alternative splicing (AS) transcriptional regulation Nicotiana tabacum L. transcription factor Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/54289ad7bb1f4d4d8733e4b2496b19ce
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spelling	oai:doaj.org-article:54289ad7bb1f4d4d8733e4b2496b19ce2021-11-08T06:50:09ZThe Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum1664-462X10.3389/fpls.2021.774829https://doaj.org/article/54289ad7bb1f4d4d8733e4b2496b19ce2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.774829/fullhttps://doaj.org/toc/1664-462XAlternative splicing (AS) occurs extensively in eukaryotes as an essential mechanism for regulating transcriptome complexity and diversity, but the AS landscape regulating potassium (K) use efficiency in plants is unclear. In this study, we performed high-throughput transcriptome sequencing of roots and shoots from allopolyploid Nicotiana tabacum under K+ deficiency. Preliminary physiological analysis showed that root system architecture was dramatically changed due to potassium deficiency and that IAA content was significantly reduced in root and shoot. AS analysis showed that a total of 28,179 genes exhibited 54,457 AS events, and 1,510 and 1,732 differentially alternatively spliced (DAS) events were identified in shoots and roots under low K+ stress. Nevertheless, only 120 DAS events occurred in both shoots and roots, implying that most DAS events were tissue-specific. Both in shoot and the root, the proportion of DAS genes in differentially expressed (DE) genes equaled that in non-DE genes, which indicated that AS might play a unique regulatory role in response to low potassium. Gene ontology analysis further indicated that transcription regulation and AS modulation worked independently in response to low K+ stress in tobacco, as their target biological processes were different. Totally 45 DAS transcription factors (TFs) were found, which were involved in 18 TF families. Five Auxin response factor (ARF) TFs were significantly DAS in root, suggesting that response to auxin was probably subject to AS regulation in the tobacco root. Our study shows that AS variation occurs extensively and has a particular regulatory mechanism under K+ deficiency in tobacco. The study also links changes in root system architecture with the changes in AS of ARF TFs, which implied the functional significance of these AS events for root growth and architecture.Bing HeBing HeLin MengLina TangWeicong QiFengqin HuYuanda LvWenjing SongFrontiers Media S.A.articlepotassiumalternative splicing (AS)transcriptional regulationNicotiana tabacum L.transcription factorPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	potassium alternative splicing (AS) transcriptional regulation Nicotiana tabacum L. transcription factor Plant culture SB1-1110
spellingShingle	potassium alternative splicing (AS) transcriptional regulation Nicotiana tabacum L. transcription factor Plant culture SB1-1110 Bing He Bing He Lin Meng Lina Tang Weicong Qi Fengqin Hu Yuanda Lv Wenjing Song The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum
description	Alternative splicing (AS) occurs extensively in eukaryotes as an essential mechanism for regulating transcriptome complexity and diversity, but the AS landscape regulating potassium (K) use efficiency in plants is unclear. In this study, we performed high-throughput transcriptome sequencing of roots and shoots from allopolyploid Nicotiana tabacum under K+ deficiency. Preliminary physiological analysis showed that root system architecture was dramatically changed due to potassium deficiency and that IAA content was significantly reduced in root and shoot. AS analysis showed that a total of 28,179 genes exhibited 54,457 AS events, and 1,510 and 1,732 differentially alternatively spliced (DAS) events were identified in shoots and roots under low K+ stress. Nevertheless, only 120 DAS events occurred in both shoots and roots, implying that most DAS events were tissue-specific. Both in shoot and the root, the proportion of DAS genes in differentially expressed (DE) genes equaled that in non-DE genes, which indicated that AS might play a unique regulatory role in response to low potassium. Gene ontology analysis further indicated that transcription regulation and AS modulation worked independently in response to low K+ stress in tobacco, as their target biological processes were different. Totally 45 DAS transcription factors (TFs) were found, which were involved in 18 TF families. Five Auxin response factor (ARF) TFs were significantly DAS in root, suggesting that response to auxin was probably subject to AS regulation in the tobacco root. Our study shows that AS variation occurs extensively and has a particular regulatory mechanism under K+ deficiency in tobacco. The study also links changes in root system architecture with the changes in AS of ARF TFs, which implied the functional significance of these AS events for root growth and architecture.
format	article
author	Bing He Bing He Lin Meng Lina Tang Weicong Qi Fengqin Hu Yuanda Lv Wenjing Song
author_facet	Bing He Bing He Lin Meng Lina Tang Weicong Qi Fengqin Hu Yuanda Lv Wenjing Song
author_sort	Bing He
title	The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum
title_short	The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum
title_full	The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum
title_fullStr	The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum
title_full_unstemmed	The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum
title_sort	landscape of alternative splicing regulating potassium use efficiency in nicotiana tabacum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/54289ad7bb1f4d4d8733e4b2496b19ce
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The Landscape of Alternative Splicing Regulating Potassium Use Efficiency in Nicotiana tabacum

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