DNA methylation dynamics in blood after hematopoietic cell transplant.

Epigenetic deregulation is considered a common hallmark of cancer. Nevertheless, recent publications have demonstrated its association with a large array of human diseases. Here, we explore the DNA methylation dynamics in blood samples during hematopoietic cell transplant and how they are affected b...

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Autores principales:	Ramon M Rodriguez, Beatriz Suarez-Alvarez, Rubén Salvanés, Manuel Muro, Pablo Martínez-Camblor, Enrique Colado, Miguel Alcoceba Sánchez, Marcos González Díaz, Agustin F Fernandez, Mario F Fraga, Carlos Lopez-Larrea
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/542b85c9329c4ca3b16eb83e6642f3cf
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spelling	oai:doaj.org-article:542b85c9329c4ca3b16eb83e6642f3cf2021-11-18T07:56:23ZDNA methylation dynamics in blood after hematopoietic cell transplant.1932-620310.1371/journal.pone.0056931https://doaj.org/article/542b85c9329c4ca3b16eb83e6642f3cf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23451113/?tool=EBIhttps://doaj.org/toc/1932-6203Epigenetic deregulation is considered a common hallmark of cancer. Nevertheless, recent publications have demonstrated its association with a large array of human diseases. Here, we explore the DNA methylation dynamics in blood samples during hematopoietic cell transplant and how they are affected by pathophysiological events during transplant evolution. We analyzed global DNA methylation in a cohort of 47 patients with allogenic transplant up to 12 months post-transplant. Recipients stably maintained the donor's global methylation levels after transplant. Nonetheless, global methylation is affected by chimerism status. Methylation analysis of promoters revealed that methylation in more than 200 genes is altered 1 month post-transplant when compared with non-pathological methylation levels in the donor. This number decreased by 6 months post-transplant. Finally, we analyzed methylation in IFN-γ, FASL, IL-10, and PRF1 and found association with the severity of the acute graft-versus-host disease. Our results provide strong evidence that methylation changes in blood are linked to underlying physiological events and demonstrate that DNA methylation analysis is a viable strategy for the study of transplantation and for development of biomarkers.Ramon M RodriguezBeatriz Suarez-AlvarezRubén SalvanésManuel MuroPablo Martínez-CamblorPablo Martínez-CamblorEnrique ColadoMiguel Alcoceba SánchezMarcos González DíazAgustin F FernandezMario F FragaCarlos Lopez-LarreaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56931 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramon M Rodriguez Beatriz Suarez-Alvarez Rubén Salvanés Manuel Muro Pablo Martínez-Camblor Pablo Martínez-Camblor Enrique Colado Miguel Alcoceba Sánchez Marcos González Díaz Agustin F Fernandez Mario F Fraga Carlos Lopez-Larrea DNA methylation dynamics in blood after hematopoietic cell transplant.
description	Epigenetic deregulation is considered a common hallmark of cancer. Nevertheless, recent publications have demonstrated its association with a large array of human diseases. Here, we explore the DNA methylation dynamics in blood samples during hematopoietic cell transplant and how they are affected by pathophysiological events during transplant evolution. We analyzed global DNA methylation in a cohort of 47 patients with allogenic transplant up to 12 months post-transplant. Recipients stably maintained the donor's global methylation levels after transplant. Nonetheless, global methylation is affected by chimerism status. Methylation analysis of promoters revealed that methylation in more than 200 genes is altered 1 month post-transplant when compared with non-pathological methylation levels in the donor. This number decreased by 6 months post-transplant. Finally, we analyzed methylation in IFN-γ, FASL, IL-10, and PRF1 and found association with the severity of the acute graft-versus-host disease. Our results provide strong evidence that methylation changes in blood are linked to underlying physiological events and demonstrate that DNA methylation analysis is a viable strategy for the study of transplantation and for development of biomarkers.
format	article
author	Ramon M Rodriguez Beatriz Suarez-Alvarez Rubén Salvanés Manuel Muro Pablo Martínez-Camblor Pablo Martínez-Camblor Enrique Colado Miguel Alcoceba Sánchez Marcos González Díaz Agustin F Fernandez Mario F Fraga Carlos Lopez-Larrea
author_facet	Ramon M Rodriguez Beatriz Suarez-Alvarez Rubén Salvanés Manuel Muro Pablo Martínez-Camblor Pablo Martínez-Camblor Enrique Colado Miguel Alcoceba Sánchez Marcos González Díaz Agustin F Fernandez Mario F Fraga Carlos Lopez-Larrea
author_sort	Ramon M Rodriguez
title	DNA methylation dynamics in blood after hematopoietic cell transplant.
title_short	DNA methylation dynamics in blood after hematopoietic cell transplant.
title_full	DNA methylation dynamics in blood after hematopoietic cell transplant.
title_fullStr	DNA methylation dynamics in blood after hematopoietic cell transplant.
title_full_unstemmed	DNA methylation dynamics in blood after hematopoietic cell transplant.
title_sort	dna methylation dynamics in blood after hematopoietic cell transplant.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/542b85c9329c4ca3b16eb83e6642f3cf
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_version_	1718422728766128128

DNA methylation dynamics in blood after hematopoietic cell transplant.

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