Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.

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Autores principales:	Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, Norbert Perrimon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/54334539e93547d7bcc9a97c1c393d4a
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spelling	oai:doaj.org-article:54334539e93547d7bcc9a97c1c393d4a2021-11-28T12:35:07ZBioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos10.1038/s41467-021-27129-32041-1723https://doaj.org/article/54334539e93547d7bcc9a97c1c393d4a2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27129-3https://doaj.org/toc/2041-1723Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.Raghuvir ViswanathaEnzo MameliJonathan RodigerPierre MerckaertFabiana Feitosa-SuntheimerTonya M. ColpittsStephanie E. MohrYanhui HuNorbert PerrimonNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Raghuvir Viswanatha Enzo Mameli Jonathan Rodiger Pierre Merckaert Fabiana Feitosa-Suntheimer Tonya M. Colpitts Stephanie E. Mohr Yanhui Hu Norbert Perrimon Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
description	Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.
format	article
author	Raghuvir Viswanatha Enzo Mameli Jonathan Rodiger Pierre Merckaert Fabiana Feitosa-Suntheimer Tonya M. Colpitts Stephanie E. Mohr Yanhui Hu Norbert Perrimon
author_facet	Raghuvir Viswanatha Enzo Mameli Jonathan Rodiger Pierre Merckaert Fabiana Feitosa-Suntheimer Tonya M. Colpitts Stephanie E. Mohr Yanhui Hu Norbert Perrimon
author_sort	Raghuvir Viswanatha
title	Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
title_short	Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
title_full	Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
title_fullStr	Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
title_full_unstemmed	Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
title_sort	bioinformatic and cell-based tools for pooled crispr knockout screening in mosquitos
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/54334539e93547d7bcc9a97c1c393d4a
work_keys_str_mv	AT raghuvirviswanatha bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT enzomameli bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT jonathanrodiger bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT pierremerckaert bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT fabianafeitosasuntheimer bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT tonyamcolpitts bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT stephanieemohr bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT yanhuihu bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos AT norbertperrimon bioinformaticandcellbasedtoolsforpooledcrisprknockoutscreeninginmosquitos
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Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

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