Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Forward genetic approaches such as CRISPR screens are powerful ways to identify essential genes and those that influence host-pathogen interactions. Here the authors design a bioinformatics portal for sgRNA design and a recombination-mediated cassette system for delivery into mosquito cell lines.

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Autores principales: Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M. Colpitts, Stephanie E. Mohr, Yanhui Hu, Norbert Perrimon
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/54334539e93547d7bcc9a97c1c393d4a
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