Establishment of a mouse model of skin-dominant chronic graft-versus-host disease

Objective To construct a mouse model of skin-dominant chronic graft-versus-host disease (cGVHD). Methods BALB/c mice were irradiated with lethal dose and then received a mixture of bone marrow and spleen cells transplantation from B10.D2 donor mice. Then the clinical manifestations, pathological fe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	WANG Weihao, YANG Shijie, DU Yuxuan, WANG Xiaoqi, HONG Tao
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Office of Journal of Third Military Medical University 2021
Materias:	chronic graft-versus-host disease skin liver ung t lymphocytes Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/543fa426289e4138bf22cb294d1ba16e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:543fa426289e4138bf22cb294d1ba16e
record_format	dspace
spelling	oai:doaj.org-article:543fa426289e4138bf22cb294d1ba16e2021-11-12T04:32:20ZEstablishment of a mouse model of skin-dominant chronic graft-versus-host disease10.16016/j.1000-5404.2021080601000-5404https://doaj.org/article/543fa426289e4138bf22cb294d1ba16e2021-11-01T00:00:00Zhttp://aammt.tmmu.edu.cn/Upload/rhtml/202108060.htmhttps://doaj.org/toc/1000-5404 Objective To construct a mouse model of skin-dominant chronic graft-versus-host disease (cGVHD). Methods BALB/c mice were irradiated with lethal dose and then received a mixture of bone marrow and spleen cells transplantation from B10.D2 donor mice. Then the clinical manifestations, pathological features of the mice and cell subsets of targeted organs were observed to evaluate the establishment of model. Results The clinical manifestations of the model mice mainly involved skin depilation, sclerosis, dandruff, scab, as well as arched back and joint stiffness due to skin shrinkage and sclerosis. The clinicopathological scores were increased with the progression of cGVHD, while the model mice showed no obvious manifestations of intestinal cGVHD. Moreover, increase of collagen deposition and decrease or even disappearance of hair follicles was observed on the histopathologic sections of skin, while other targeted organs showed no major alterations. T cell subsets analysis indicated that Th1 cells, CD4+IL-13+T cells and Tc1 cells were closely related to the disease progression. Conclusion The skin-dominant cGVHD mouse model is successfully established, with skin damage and fibrosis as main pathological changes.WANG WeihaoYANG ShijieDU YuxuanWANG XiaoqiHONG TaoEditorial Office of Journal of Third Military Medical Universityarticlechronic graft-versus-host diseaseskinliverungt lymphocytesMedicine (General)R5-920ZHDi-san junyi daxue xuebao, Vol 43, Iss 21, Pp 2286-2293 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	chronic graft-versus-host disease skin liver ung t lymphocytes Medicine (General) R5-920
spellingShingle	chronic graft-versus-host disease skin liver ung t lymphocytes Medicine (General) R5-920 WANG Weihao YANG Shijie DU Yuxuan WANG Xiaoqi HONG Tao Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
description	Objective To construct a mouse model of skin-dominant chronic graft-versus-host disease (cGVHD). Methods BALB/c mice were irradiated with lethal dose and then received a mixture of bone marrow and spleen cells transplantation from B10.D2 donor mice. Then the clinical manifestations, pathological features of the mice and cell subsets of targeted organs were observed to evaluate the establishment of model. Results The clinical manifestations of the model mice mainly involved skin depilation, sclerosis, dandruff, scab, as well as arched back and joint stiffness due to skin shrinkage and sclerosis. The clinicopathological scores were increased with the progression of cGVHD, while the model mice showed no obvious manifestations of intestinal cGVHD. Moreover, increase of collagen deposition and decrease or even disappearance of hair follicles was observed on the histopathologic sections of skin, while other targeted organs showed no major alterations. T cell subsets analysis indicated that Th1 cells, CD4+IL-13+T cells and Tc1 cells were closely related to the disease progression. Conclusion The skin-dominant cGVHD mouse model is successfully established, with skin damage and fibrosis as main pathological changes.
format	article
author	WANG Weihao YANG Shijie DU Yuxuan WANG Xiaoqi HONG Tao
author_facet	WANG Weihao YANG Shijie DU Yuxuan WANG Xiaoqi HONG Tao
author_sort	WANG Weihao
title	Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
title_short	Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
title_full	Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
title_fullStr	Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
title_full_unstemmed	Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
title_sort	establishment of a mouse model of skin-dominant chronic graft-versus-host disease
publisher	Editorial Office of Journal of Third Military Medical University
publishDate	2021
url	https://doaj.org/article/543fa426289e4138bf22cb294d1ba16e
work_keys_str_mv	AT wangweihao establishmentofamousemodelofskindominantchronicgraftversushostdisease AT yangshijie establishmentofamousemodelofskindominantchronicgraftversushostdisease AT duyuxuan establishmentofamousemodelofskindominantchronicgraftversushostdisease AT wangxiaoqi establishmentofamousemodelofskindominantchronicgraftversushostdisease AT hongtao establishmentofamousemodelofskindominantchronicgraftversushostdisease
_version_	1718431290878853120

Establishment of a mouse model of skin-dominant chronic graft-versus-host disease

Ejemplares similares