Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair

Sensory neurons with cell bodies in dorsal root ganglia (DRG) represent a useful model to study axon regeneration. Whereas regeneration and functional recovery occurs after peripheral nerve injury, spinal cord injury or dorsal root injury is not followed by regenerative outcomes. Regeneration of sen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Oshri Avraham, Rui Feng, Eric Edward Ewan, Justin Rustenhoven, Guoyan Zhao, Valeria Cavalli
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	axon regeneration satellite glial cells nerve repair single-cell RNA sequencing dorsal root ganglion Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5442f1c8cdd94e88a642340c71c4afe2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5442f1c8cdd94e88a642340c71c4afe2
record_format	dspace
spelling	oai:doaj.org-article:5442f1c8cdd94e88a642340c71c4afe22021-11-25T10:27:50ZProfiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair10.7554/eLife.684572050-084Xe68457https://doaj.org/article/5442f1c8cdd94e88a642340c71c4afe22021-09-01T00:00:00Zhttps://elifesciences.org/articles/68457https://doaj.org/toc/2050-084XSensory neurons with cell bodies in dorsal root ganglia (DRG) represent a useful model to study axon regeneration. Whereas regeneration and functional recovery occurs after peripheral nerve injury, spinal cord injury or dorsal root injury is not followed by regenerative outcomes. Regeneration of sensory axons in peripheral nerves is not entirely cell autonomous. Whether the DRG microenvironment influences the different regenerative capacities after injury to peripheral or central axons remains largely unknown. To answer this question, we performed a single-cell transcriptional profiling of mouse DRG in response to peripheral (sciatic nerve crush) and central axon injuries (dorsal root crush and spinal cord injury). Each cell type responded differently to the three types of injuries. All injuries increased the proportion of a cell type that shares features of both immune cells and glial cells. A distinct subset of satellite glial cells (SGC) appeared specifically in response to peripheral nerve injury. Activation of the PPARα signaling pathway in SGC, which promotes axon regeneration after peripheral nerve injury, failed to occur after central axon injuries. Treatment with the FDA-approved PPARα agonist fenofibrate increased axon regeneration after dorsal root injury. This study provides a map of the distinct DRG microenvironment responses to peripheral and central injuries at the single-cell level and highlights that manipulating non-neuronal cells could lead to avenues to promote functional recovery after CNS injuries or disease.Oshri AvrahamRui FengEric Edward EwanJustin RustenhovenGuoyan ZhaoValeria CavallieLife Sciences Publications Ltdarticleaxon regenerationsatellite glial cellsnerve repairsingle-cell RNA sequencingdorsal root ganglionMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	axon regeneration satellite glial cells nerve repair single-cell RNA sequencing dorsal root ganglion Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	axon regeneration satellite glial cells nerve repair single-cell RNA sequencing dorsal root ganglion Medicine R Science Q Biology (General) QH301-705.5 Oshri Avraham Rui Feng Eric Edward Ewan Justin Rustenhoven Guoyan Zhao Valeria Cavalli Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
description	Sensory neurons with cell bodies in dorsal root ganglia (DRG) represent a useful model to study axon regeneration. Whereas regeneration and functional recovery occurs after peripheral nerve injury, spinal cord injury or dorsal root injury is not followed by regenerative outcomes. Regeneration of sensory axons in peripheral nerves is not entirely cell autonomous. Whether the DRG microenvironment influences the different regenerative capacities after injury to peripheral or central axons remains largely unknown. To answer this question, we performed a single-cell transcriptional profiling of mouse DRG in response to peripheral (sciatic nerve crush) and central axon injuries (dorsal root crush and spinal cord injury). Each cell type responded differently to the three types of injuries. All injuries increased the proportion of a cell type that shares features of both immune cells and glial cells. A distinct subset of satellite glial cells (SGC) appeared specifically in response to peripheral nerve injury. Activation of the PPARα signaling pathway in SGC, which promotes axon regeneration after peripheral nerve injury, failed to occur after central axon injuries. Treatment with the FDA-approved PPARα agonist fenofibrate increased axon regeneration after dorsal root injury. This study provides a map of the distinct DRG microenvironment responses to peripheral and central injuries at the single-cell level and highlights that manipulating non-neuronal cells could lead to avenues to promote functional recovery after CNS injuries or disease.
format	article
author	Oshri Avraham Rui Feng Eric Edward Ewan Justin Rustenhoven Guoyan Zhao Valeria Cavalli
author_facet	Oshri Avraham Rui Feng Eric Edward Ewan Justin Rustenhoven Guoyan Zhao Valeria Cavalli
author_sort	Oshri Avraham
title	Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
title_short	Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
title_full	Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
title_fullStr	Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
title_full_unstemmed	Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
title_sort	profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/5442f1c8cdd94e88a642340c71c4afe2
work_keys_str_mv	AT oshriavraham profilingsensoryneuronmicroenvironmentafterperipheralandcentralaxoninjuryrevealskeypathwaysforneuralrepair AT ruifeng profilingsensoryneuronmicroenvironmentafterperipheralandcentralaxoninjuryrevealskeypathwaysforneuralrepair AT ericedwardewan profilingsensoryneuronmicroenvironmentafterperipheralandcentralaxoninjuryrevealskeypathwaysforneuralrepair AT justinrustenhoven profilingsensoryneuronmicroenvironmentafterperipheralandcentralaxoninjuryrevealskeypathwaysforneuralrepair AT guoyanzhao profilingsensoryneuronmicroenvironmentafterperipheralandcentralaxoninjuryrevealskeypathwaysforneuralrepair AT valeriacavalli profilingsensoryneuronmicroenvironmentafterperipheralandcentralaxoninjuryrevealskeypathwaysforneuralrepair
_version_	1718413556089618432

Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair

Ejemplares similares