Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease

In the late phase of <i>Trypanosoma cruzi</i> infection, parasite persistence and an exaggerated immune response accompanied by oxidative stress play a crucial role in the genesis of Chronic Chagasic Cardiomyopathy (CCC). Current treatments (Benznidazole (BNZ) and Nifurtimox) can effect...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Denerieth Ximena Espinel-Mesa, Clara Isabel González Rugeles, Julio César Mantilla Hernández, Elena E. Stashenko, Carlos Andrés Villegas-Lanau, John Jaime Quimbaya Ramírez, Liliana Torcoroma García Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chronic Chagas disease <i>Trypanosoma cruzi</i> antioxidant immunomodulation immunohistochemistry essential oils Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/54438e7782a84e85b9b67dd0064f06bf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:54438e7782a84e85b9b67dd0064f06bf
record_format	dspace
spelling	oai:doaj.org-article:54438e7782a84e85b9b67dd0064f06bf2021-11-25T16:29:51ZImmunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease10.3390/antiox101118512076-3921https://doaj.org/article/54438e7782a84e85b9b67dd0064f06bf2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1851https://doaj.org/toc/2076-3921In the late phase of <i>Trypanosoma cruzi</i> infection, parasite persistence and an exaggerated immune response accompanied by oxidative stress play a crucial role in the genesis of Chronic Chagasic Cardiomyopathy (CCC). Current treatments (Benznidazole (BNZ) and Nifurtimox) can effect only the elimination of the parasite, but are ineffective for late stage treatment and for preventing heart damage and disease progression. In vivo trypanocidal and cardioprotective activity has been reported for <i>Lippia alba</i> essential oils (EOs), ascribed to their two major terpenes, limonene and caryophyllene oxide. To investigate the role of antioxidant and immunomodulatory mechanisms behind these properties, chronic-<i>T. cruzi</i>-infected rats were treated with oral synergistic mixtures of the aforementioned EOs. For this purpose, the EOs were optimized through limonene-enrichment fractioning and by the addition of exogenous caryophyllene oxide (LIMOX) and used alone or in combined therapy with subtherapeutic doses of BNZ (LIMOXBNZ). Clinical, toxicity, inflammatory, oxidative, and parasitological (qPCR) parameters were assessed in cardiac tissue. These therapies demonstrated meaningful antioxidant and immunomodulatory activity on markers involved in CCC pathogenesis (IFN-γ, TNF-α, IL-4, IL-10, and iNOS), which could explain their significant trypanocidal properties and their noteworthy role in preventing, and even reversing, the progression of cardiac damage in chronic Chagas disease.Denerieth Ximena Espinel-MesaClara Isabel González RugelesJulio César Mantilla HernándezElena E. StashenkoCarlos Andrés Villegas-LanauJohn Jaime Quimbaya RamírezLiliana Torcoroma García SánchezMDPI AGarticlechronic Chagas disease<i>Trypanosoma cruzi</i>antioxidantimmunomodulationimmunohistochemistryessential oilsTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1851, p 1851 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic Chagas disease <i>Trypanosoma cruzi</i> antioxidant immunomodulation immunohistochemistry essential oils Therapeutics. Pharmacology RM1-950
spellingShingle	chronic Chagas disease <i>Trypanosoma cruzi</i> antioxidant immunomodulation immunohistochemistry essential oils Therapeutics. Pharmacology RM1-950 Denerieth Ximena Espinel-Mesa Clara Isabel González Rugeles Julio César Mantilla Hernández Elena E. Stashenko Carlos Andrés Villegas-Lanau John Jaime Quimbaya Ramírez Liliana Torcoroma García Sánchez Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease
description	In the late phase of <i>Trypanosoma cruzi</i> infection, parasite persistence and an exaggerated immune response accompanied by oxidative stress play a crucial role in the genesis of Chronic Chagasic Cardiomyopathy (CCC). Current treatments (Benznidazole (BNZ) and Nifurtimox) can effect only the elimination of the parasite, but are ineffective for late stage treatment and for preventing heart damage and disease progression. In vivo trypanocidal and cardioprotective activity has been reported for <i>Lippia alba</i> essential oils (EOs), ascribed to their two major terpenes, limonene and caryophyllene oxide. To investigate the role of antioxidant and immunomodulatory mechanisms behind these properties, chronic-<i>T. cruzi</i>-infected rats were treated with oral synergistic mixtures of the aforementioned EOs. For this purpose, the EOs were optimized through limonene-enrichment fractioning and by the addition of exogenous caryophyllene oxide (LIMOX) and used alone or in combined therapy with subtherapeutic doses of BNZ (LIMOXBNZ). Clinical, toxicity, inflammatory, oxidative, and parasitological (qPCR) parameters were assessed in cardiac tissue. These therapies demonstrated meaningful antioxidant and immunomodulatory activity on markers involved in CCC pathogenesis (IFN-γ, TNF-α, IL-4, IL-10, and iNOS), which could explain their significant trypanocidal properties and their noteworthy role in preventing, and even reversing, the progression of cardiac damage in chronic Chagas disease.
format	article
author	Denerieth Ximena Espinel-Mesa Clara Isabel González Rugeles Julio César Mantilla Hernández Elena E. Stashenko Carlos Andrés Villegas-Lanau John Jaime Quimbaya Ramírez Liliana Torcoroma García Sánchez
author_facet	Denerieth Ximena Espinel-Mesa Clara Isabel González Rugeles Julio César Mantilla Hernández Elena E. Stashenko Carlos Andrés Villegas-Lanau John Jaime Quimbaya Ramírez Liliana Torcoroma García Sánchez
author_sort	Denerieth Ximena Espinel-Mesa
title	Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease
title_short	Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease
title_full	Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease
title_fullStr	Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease
title_full_unstemmed	Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease
title_sort	immunomodulation and antioxidant activities as possible trypanocidal and cardioprotective mechanisms of major terpenes from <i>lippia alba</i> essential oils in an experimental model of chronic chagas disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/54438e7782a84e85b9b67dd0064f06bf
work_keys_str_mv	AT deneriethximenaespinelmesa immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease AT claraisabelgonzalezrugeles immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease AT juliocesarmantillahernandez immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease AT elenaestashenko immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease AT carlosandresvillegaslanau immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease AT johnjaimequimbayaramirez immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease AT lilianatorcoromagarciasanchez immunomodulationandantioxidantactivitiesaspossibletrypanocidalandcardioprotectivemechanismsofmajorterpenesfromilippiaalbaiessentialoilsinanexperimentalmodelofchronicchagasdisease
_version_	1718413135899000832

Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from <i>Lippia alba</i> Essential Oils in an Experimental Model of Chronic Chagas Disease

Ejemplares similares