Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings

Abstract Cell sheet technology is becoming increasingly popular in tissue engineering and regenerative medicine, due to integrity into versatile organ and manageable cell and tissue type from the bank, and no needs of large volume organ for transplantation. Cell sheets have still a room to resolve t...

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Autores principales:	Miso Yang, Eunah Kang, Jong wook Shin, Jinkee Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/544607a2854e45febe9032f82f3e4e0e
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spelling	oai:doaj.org-article:544607a2854e45febe9032f82f3e4e0e2021-12-02T12:31:52ZSurface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings10.1038/s41598-017-04746-x2045-2322https://doaj.org/article/544607a2854e45febe9032f82f3e4e0e2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04746-xhttps://doaj.org/toc/2045-2322Abstract Cell sheet technology is becoming increasingly popular in tissue engineering and regenerative medicine, due to integrity into versatile organ and manageable cell and tissue type from the bank, and no needs of large volume organ for transplantation. Cell sheets have still a room to resolve the mechanical resistance under load-bearing occasion, easy translocation into organ, and prompt shape modulation for regular application in vivo. Herein, a layer-by-layer (LbL) assembly of nanometer scaled film coating method was introduced to inter-planar cell sheet for multilayered cell sheet (M1) and a single cell before sheet formation (M2). Nano-films with collagen and alginate increased mechanical property of cell sheets without altering cell functions, viability, and proliferation. The moduli of triple layered cell sheet (M1) and (M2) were critically enhanced to 109% and 104%, compared to uncoated cell sheet (CON) with mono-layer, while modulus of CON with triple-layers were increased to 43%. LbL assembly to cell sheets offers increased modulus allowing cell sheet engineering to become a potential strategy under load-bearing environment.Miso YangEunah KangJong wook ShinJinkee HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miso Yang Eunah Kang Jong wook Shin Jinkee Hong Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings
description	Abstract Cell sheet technology is becoming increasingly popular in tissue engineering and regenerative medicine, due to integrity into versatile organ and manageable cell and tissue type from the bank, and no needs of large volume organ for transplantation. Cell sheets have still a room to resolve the mechanical resistance under load-bearing occasion, easy translocation into organ, and prompt shape modulation for regular application in vivo. Herein, a layer-by-layer (LbL) assembly of nanometer scaled film coating method was introduced to inter-planar cell sheet for multilayered cell sheet (M1) and a single cell before sheet formation (M2). Nano-films with collagen and alginate increased mechanical property of cell sheets without altering cell functions, viability, and proliferation. The moduli of triple layered cell sheet (M1) and (M2) were critically enhanced to 109% and 104%, compared to uncoated cell sheet (CON) with mono-layer, while modulus of CON with triple-layers were increased to 43%. LbL assembly to cell sheets offers increased modulus allowing cell sheet engineering to become a potential strategy under load-bearing environment.
format	article
author	Miso Yang Eunah Kang Jong wook Shin Jinkee Hong
author_facet	Miso Yang Eunah Kang Jong wook Shin Jinkee Hong
author_sort	Miso Yang
title	Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings
title_short	Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings
title_full	Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings
title_fullStr	Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings
title_full_unstemmed	Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings
title_sort	surface engineering for mechanical enhancement of cell sheet by nano-coatings
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/544607a2854e45febe9032f82f3e4e0e
work_keys_str_mv	AT misoyang surfaceengineeringformechanicalenhancementofcellsheetbynanocoatings AT eunahkang surfaceengineeringformechanicalenhancementofcellsheetbynanocoatings AT jongwookshin surfaceengineeringformechanicalenhancementofcellsheetbynanocoatings AT jinkeehong surfaceengineeringformechanicalenhancementofcellsheetbynanocoatings
_version_	1718394214600933376

Surface Engineering for Mechanical Enhancement of Cell Sheet by Nano-Coatings

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