Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3

Abstract Pancreatic islet transplantation is a promising therapy for patients with type 1 diabetes. However, the duration of long-term graft survival is limited due to inflammatory as well as non-inflammatory processes and routine clinical tests are not suitable to monitor islet survival. 111In-exen...

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Autores principales:	Wael A. Eter, Inge Van der Kroon, Karolina Andralojc, Mijke Buitinga, Stefanie M. A. Willekens, Cathelijne Frielink, Desiree Bos, Lieke Joosten, Otto C. Boerman, Maarten Brom, Martin Gotthardt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54496705e6054b69a78f30ff906bdda1
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spelling	oai:doaj.org-article:54496705e6054b69a78f30ff906bdda12021-12-02T11:41:19ZNon-invasive in vivo determination of viable islet graft volume by 111In-exendin-310.1038/s41598-017-07815-32045-2322https://doaj.org/article/54496705e6054b69a78f30ff906bdda12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07815-3https://doaj.org/toc/2045-2322Abstract Pancreatic islet transplantation is a promising therapy for patients with type 1 diabetes. However, the duration of long-term graft survival is limited due to inflammatory as well as non-inflammatory processes and routine clinical tests are not suitable to monitor islet survival. 111In-exendin-SPECT (single photon emission computed tomography) is a promising method to non-invasively image islets after transplantation and has the potential to help improve the clinical outcome. Whether 111In-exendin-SPECT allows detecting small differences in beta-cell mass (BCM) and measuring the actual volume of islets that were successfully engrafted has yet to be demonstrated. Here, we evaluated the performance of 111In-exendin-SPECT using an intramuscular islet transplantation model in C3H mice. In vivo imaging of animals transplanted with 50, 100, 200, 400 and 800 islets revealed an excellent linear correlation between SPECT quantification of 111In-exendin uptake and insulin-positive area of islet transplants, demonstrating that 111In-exendin-SPECT specifically and accurately measures BCM. The high sensitivity of the method allowed measuring small differences in graft volumes, including grafts that contained less than 50 islets. The presented method is reliable, convenient and holds great potential for non-invasive monitoring of BCM after islet transplantation in humans.Wael A. EterInge Van der KroonKarolina AndralojcMijke BuitingaStefanie M. A. WillekensCathelijne FrielinkDesiree BosLieke JoostenOtto C. BoermanMaarten BromMartin GotthardtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wael A. Eter Inge Van der Kroon Karolina Andralojc Mijke Buitinga Stefanie M. A. Willekens Cathelijne Frielink Desiree Bos Lieke Joosten Otto C. Boerman Maarten Brom Martin Gotthardt Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3
description	Abstract Pancreatic islet transplantation is a promising therapy for patients with type 1 diabetes. However, the duration of long-term graft survival is limited due to inflammatory as well as non-inflammatory processes and routine clinical tests are not suitable to monitor islet survival. 111In-exendin-SPECT (single photon emission computed tomography) is a promising method to non-invasively image islets after transplantation and has the potential to help improve the clinical outcome. Whether 111In-exendin-SPECT allows detecting small differences in beta-cell mass (BCM) and measuring the actual volume of islets that were successfully engrafted has yet to be demonstrated. Here, we evaluated the performance of 111In-exendin-SPECT using an intramuscular islet transplantation model in C3H mice. In vivo imaging of animals transplanted with 50, 100, 200, 400 and 800 islets revealed an excellent linear correlation between SPECT quantification of 111In-exendin uptake and insulin-positive area of islet transplants, demonstrating that 111In-exendin-SPECT specifically and accurately measures BCM. The high sensitivity of the method allowed measuring small differences in graft volumes, including grafts that contained less than 50 islets. The presented method is reliable, convenient and holds great potential for non-invasive monitoring of BCM after islet transplantation in humans.
format	article
author	Wael A. Eter Inge Van der Kroon Karolina Andralojc Mijke Buitinga Stefanie M. A. Willekens Cathelijne Frielink Desiree Bos Lieke Joosten Otto C. Boerman Maarten Brom Martin Gotthardt
author_facet	Wael A. Eter Inge Van der Kroon Karolina Andralojc Mijke Buitinga Stefanie M. A. Willekens Cathelijne Frielink Desiree Bos Lieke Joosten Otto C. Boerman Maarten Brom Martin Gotthardt
author_sort	Wael A. Eter
title	Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3
title_short	Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3
title_full	Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3
title_fullStr	Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3
title_full_unstemmed	Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3
title_sort	non-invasive in vivo determination of viable islet graft volume by 111in-exendin-3
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/54496705e6054b69a78f30ff906bdda1
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Non-invasive in vivo determination of viable islet graft volume by 111In-exendin-3

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