Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda

ABSTRACT: Real-time polymerase chain reaction (RT-PCR) remains the gold standard for detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This study tested the performance of a pooled testing strategy for RT-PCR and its cost-effectiveness. In total, 1280 leftover respiratory sa...

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Autores principales:	Naghib Bogere, Felix Bongomin, Andrew Katende, Kenneth Ssebambulidde, Willy Ssengooba, Henry Ssenfuka, Edgar Kigozi, Samuel Biraro, David P. Kateete, Irene Andia-Biraro
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	COVID-19 Pooled testing RT-PCR SARS-CoV-2 LMIC Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/5449e3c80f9540ec80bec7bd37bb76a1
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spelling	oai:doaj.org-article:5449e3c80f9540ec80bec7bd37bb76a12021-11-20T04:56:18ZPerformance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda1201-971210.1016/j.ijid.2021.10.038https://doaj.org/article/5449e3c80f9540ec80bec7bd37bb76a12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1201971221008341https://doaj.org/toc/1201-9712ABSTRACT: Real-time polymerase chain reaction (RT-PCR) remains the gold standard for detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This study tested the performance of a pooled testing strategy for RT-PCR and its cost-effectiveness. In total, 1280 leftover respiratory samples collected between 19 April and 6 May 2021 were tested in 128 pools of 10 samples each, out of which 16 pools were positive. The positivity rate of the unpooled samples was 1.9% (24/1280). After parallel testing using the individual and pooled testing strategies, positive agreement was 100% and negative agreement was 99.8%. The overall median cycle threshold (Ct) value of the unpooled samples was 29.8 (interquartile range 22.3–34.3). Pools that remained positive when compared with the results of individual samples had lower median Ct values compared with those that turned out to be negative (28.8 versus 34.8; P=0.0.035). Pooled testing reduced the cost >4-fold. Pooled testing may be a more cost-effective approach to diagnose SARS-CoV-2 in resource-limited settings without compromising diagnostic performance.Naghib BogereFelix BongominAndrew KatendeKenneth SsebambuliddeWilly SsengoobaHenry SsenfukaEdgar KigoziSamuel BiraroDavid P. KateeteIrene Andia-BiraroElsevierarticleCOVID-19Pooled testingRT-PCRSARS-CoV-2LMICInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 113, Iss , Pp 355-358 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 Pooled testing RT-PCR SARS-CoV-2 LMIC Infectious and parasitic diseases RC109-216
spellingShingle	COVID-19 Pooled testing RT-PCR SARS-CoV-2 LMIC Infectious and parasitic diseases RC109-216 Naghib Bogere Felix Bongomin Andrew Katende Kenneth Ssebambulidde Willy Ssengooba Henry Ssenfuka Edgar Kigozi Samuel Biraro David P. Kateete Irene Andia-Biraro Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda
description	ABSTRACT: Real-time polymerase chain reaction (RT-PCR) remains the gold standard for detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This study tested the performance of a pooled testing strategy for RT-PCR and its cost-effectiveness. In total, 1280 leftover respiratory samples collected between 19 April and 6 May 2021 were tested in 128 pools of 10 samples each, out of which 16 pools were positive. The positivity rate of the unpooled samples was 1.9% (24/1280). After parallel testing using the individual and pooled testing strategies, positive agreement was 100% and negative agreement was 99.8%. The overall median cycle threshold (Ct) value of the unpooled samples was 29.8 (interquartile range 22.3–34.3). Pools that remained positive when compared with the results of individual samples had lower median Ct values compared with those that turned out to be negative (28.8 versus 34.8; P=0.0.035). Pooled testing reduced the cost >4-fold. Pooled testing may be a more cost-effective approach to diagnose SARS-CoV-2 in resource-limited settings without compromising diagnostic performance.
format	article
author	Naghib Bogere Felix Bongomin Andrew Katende Kenneth Ssebambulidde Willy Ssengooba Henry Ssenfuka Edgar Kigozi Samuel Biraro David P. Kateete Irene Andia-Biraro
author_facet	Naghib Bogere Felix Bongomin Andrew Katende Kenneth Ssebambulidde Willy Ssengooba Henry Ssenfuka Edgar Kigozi Samuel Biraro David P. Kateete Irene Andia-Biraro
author_sort	Naghib Bogere
title	Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda
title_short	Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda
title_full	Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda
title_fullStr	Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda
title_full_unstemmed	Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda
title_sort	performance and cost-effectiveness of a pooled testing strategy for sars-cov-2 using real-time polymerase chain reaction in uganda
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5449e3c80f9540ec80bec7bd37bb76a1
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Performance and cost-effectiveness of a pooled testing strategy for SARS-CoV-2 using real-time polymerase chain reaction in Uganda

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