On-demand synthesis of phosphoramidites

The poor bench stability of phosphoramidites is a drawback for fast automised chemical oligonucleotide synthesis. Here, the authors report a method for on-demand flow synthesis of phosphoramidites within short reaction times, in near-quantitative yields and sufficient purity for integration with DNA...

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Autores principales:	Alexander F. Sandahl, Thuy J. D. Nguyen, Rikke A. Hansen, Martin B. Johansen, Troels Skrydstrup, Kurt V. Gothelf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/544d536eae8a41adb25c5f2ddb62b424
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spelling	oai:doaj.org-article:544d536eae8a41adb25c5f2ddb62b4242021-12-02T14:49:23ZOn-demand synthesis of phosphoramidites10.1038/s41467-021-22945-z2041-1723https://doaj.org/article/544d536eae8a41adb25c5f2ddb62b4242021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22945-zhttps://doaj.org/toc/2041-1723The poor bench stability of phosphoramidites is a drawback for fast automised chemical oligonucleotide synthesis. Here, the authors report a method for on-demand flow synthesis of phosphoramidites within short reaction times, in near-quantitative yields and sufficient purity for integration with DNA synthesizers.Alexander F. SandahlThuy J. D. NguyenRikke A. HansenMartin B. JohansenTroels SkrydstrupKurt V. GothelfNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexander F. Sandahl Thuy J. D. Nguyen Rikke A. Hansen Martin B. Johansen Troels Skrydstrup Kurt V. Gothelf On-demand synthesis of phosphoramidites
description	The poor bench stability of phosphoramidites is a drawback for fast automised chemical oligonucleotide synthesis. Here, the authors report a method for on-demand flow synthesis of phosphoramidites within short reaction times, in near-quantitative yields and sufficient purity for integration with DNA synthesizers.
format	article
author	Alexander F. Sandahl Thuy J. D. Nguyen Rikke A. Hansen Martin B. Johansen Troels Skrydstrup Kurt V. Gothelf
author_facet	Alexander F. Sandahl Thuy J. D. Nguyen Rikke A. Hansen Martin B. Johansen Troels Skrydstrup Kurt V. Gothelf
author_sort	Alexander F. Sandahl
title	On-demand synthesis of phosphoramidites
title_short	On-demand synthesis of phosphoramidites
title_full	On-demand synthesis of phosphoramidites
title_fullStr	On-demand synthesis of phosphoramidites
title_full_unstemmed	On-demand synthesis of phosphoramidites
title_sort	on-demand synthesis of phosphoramidites
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/544d536eae8a41adb25c5f2ddb62b424
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On-demand synthesis of phosphoramidites

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