Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms

Abstract This study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order...

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Autores principales:	Susanne Krause, Andreas Bremges, Philipp C. Münch, Alice C. McHardy, Johannes Gescher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54555642c8f34a30b282b8e689a8cd4e
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spelling	oai:doaj.org-article:54555642c8f34a30b282b8e689a8cd4e2021-12-02T11:40:12ZCharacterisation of a stable laboratory co-culture of acidophilic nanoorganisms10.1038/s41598-017-03315-62045-2322https://doaj.org/article/54555642c8f34a30b282b8e689a8cd4e2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03315-6https://doaj.org/toc/2045-2322Abstract This study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated by this microorganism and the ARMAN. The third archaeon in the community seems to be present in minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum. The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by the archaea of the community. Microscopic analysis revealed that growth of the ARMAN is supported by the formation of cell aggregates. These might enable feeding of the ARMAN by or on other community members.Susanne KrauseAndreas BremgesPhilipp C. MünchAlice C. McHardyJohannes GescherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susanne Krause Andreas Bremges Philipp C. Münch Alice C. McHardy Johannes Gescher Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
description	Abstract This study describes the laboratory cultivation of ARMAN (Archaeal Richmond Mine Acidophilic Nanoorganisms). After 2.5 years of successive transfers in an anoxic medium containing ferric sulfate as an electron acceptor, a consortium was attained that is comprised of two members of the order Thermoplasmatales, a member of a proposed ARMAN group, as well as a fungus. The 16S rRNA identity of one archaeon is only 91.6% compared to the most closely related isolate Thermogymnomonas acidicola. Hence, this organism is the first member of a new genus. The enrichment culture is dominated by this microorganism and the ARMAN. The third archaeon in the community seems to be present in minor quantities and has a 100% 16S rRNA identity to the recently isolated Cuniculiplasma divulgatum. The enriched ARMAN species is most probably incapable of sugar metabolism because the key genes for sugar catabolism and anabolism could not be identified in the metagenome. Metatranscriptomic analysis suggests that the TCA cycle funneled with amino acids is the main metabolic pathway used by the archaea of the community. Microscopic analysis revealed that growth of the ARMAN is supported by the formation of cell aggregates. These might enable feeding of the ARMAN by or on other community members.
format	article
author	Susanne Krause Andreas Bremges Philipp C. Münch Alice C. McHardy Johannes Gescher
author_facet	Susanne Krause Andreas Bremges Philipp C. Münch Alice C. McHardy Johannes Gescher
author_sort	Susanne Krause
title	Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
title_short	Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
title_full	Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
title_fullStr	Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
title_full_unstemmed	Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
title_sort	characterisation of a stable laboratory co-culture of acidophilic nanoorganisms
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/54555642c8f34a30b282b8e689a8cd4e
work_keys_str_mv	AT susannekrause characterisationofastablelaboratorycocultureofacidophilicnanoorganisms AT andreasbremges characterisationofastablelaboratorycocultureofacidophilicnanoorganisms AT philippcmunch characterisationofastablelaboratorycocultureofacidophilicnanoorganisms AT alicecmchardy characterisationofastablelaboratorycocultureofacidophilicnanoorganisms AT johannesgescher characterisationofastablelaboratorycocultureofacidophilicnanoorganisms
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Characterisation of a stable laboratory co-culture of acidophilic nanoorganisms

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