<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1

ABSTRACT Saccharomyces cerevisiae has been frequently used to study biogenesis, functionality, and intracellular transport of various renal proteins, including ion channels, solute transporters, and aquaporins. Specific mutations in genes encoding most of these renal proteins affect kidney function...

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Autores principales:	Hasib A. M. Sarder, Xiaobing Li, Charlotta Funaya, Emmanuelle Cordat, Manfred J. Schmitt, Björn Becker
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	S. cerevisiae model organism kidney anion exchanger 1 (kAE1) heterologous expression electron microscopy pH Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/545a465098ae4202b8c81d0e6afeeb9f
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spelling	oai:doaj.org-article:545a465098ae4202b8c81d0e6afeeb9f2021-11-15T15:27:52Z<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 110.1128/mSphere.00802-192379-5042https://doaj.org/article/545a465098ae4202b8c81d0e6afeeb9f2020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00802-19https://doaj.org/toc/2379-5042ABSTRACT Saccharomyces cerevisiae has been frequently used to study biogenesis, functionality, and intracellular transport of various renal proteins, including ion channels, solute transporters, and aquaporins. Specific mutations in genes encoding most of these renal proteins affect kidney function in such a way that various disease phenotypes ultimately occur. In this context, human kidney anion exchanger 1 (kAE1) represents an important bicarbonate/chloride exchanger which maintains the acid-base homeostasis in the human body. Malfunctions in kAE1 lead to a pathological phenotype known as distal renal tubular acidosis (dRTA). Here, we evaluated the potential of baker's yeast as a model system to investigate different cellular aspects of kAE1 physiology. For the first time, we successfully expressed yeast codon-optimized full-length versions of tagged and untagged wild-type kAE1 and demonstrated their partial localization at the yeast plasma membrane (PM). Finally, pH and chloride measurements further suggest biological activity of full-length kAE1, emphasizing the potential of S. cerevisiae as a model system for studying trafficking, activity, and/or degradation of mammalian ion channels and transporters such as kAE1 in the future. IMPORTANCE Distal renal tubular acidosis (dRTA) is a common kidney dysfunction characterized by impaired acid secretion via urine. Previous studies revealed that α-intercalated cells of dRTA patients express mutated forms of human kidney anion exchanger 1 (kAE1) which result in inefficient plasma membrane targeting or diminished expression levels of kAE1. However, the precise dRTA-causing processes are inadequately understood, and alternative model systems are helpful tools to address kAE1-related questions in a fast and inexpensive way. In contrast to a previous study, we successfully expressed full-length kAE1 in Saccharomyces cerevisiae. Using advanced microscopy techniques as well as different biochemical and functionality assays, plasma membrane localization and biological activity were confirmed for the heterologously expressed anion transporter. These findings represent first important steps to use the potential of yeast as a model organism for studying trafficking, activity, and degradation of kAE1 and its mutant variants in the future.Hasib A. M. SarderXiaobing LiCharlotta FunayaEmmanuelle CordatManfred J. SchmittBjörn BeckerAmerican Society for MicrobiologyarticleS. cerevisiaemodel organismkidney anion exchanger 1 (kAE1)heterologous expressionelectron microscopypHMicrobiologyQR1-502ENmSphere, Vol 5, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	S. cerevisiae model organism kidney anion exchanger 1 (kAE1) heterologous expression electron microscopy pH Microbiology QR1-502
spellingShingle	S. cerevisiae model organism kidney anion exchanger 1 (kAE1) heterologous expression electron microscopy pH Microbiology QR1-502 Hasib A. M. Sarder Xiaobing Li Charlotta Funaya Emmanuelle Cordat Manfred J. Schmitt Björn Becker <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1
description	ABSTRACT Saccharomyces cerevisiae has been frequently used to study biogenesis, functionality, and intracellular transport of various renal proteins, including ion channels, solute transporters, and aquaporins. Specific mutations in genes encoding most of these renal proteins affect kidney function in such a way that various disease phenotypes ultimately occur. In this context, human kidney anion exchanger 1 (kAE1) represents an important bicarbonate/chloride exchanger which maintains the acid-base homeostasis in the human body. Malfunctions in kAE1 lead to a pathological phenotype known as distal renal tubular acidosis (dRTA). Here, we evaluated the potential of baker's yeast as a model system to investigate different cellular aspects of kAE1 physiology. For the first time, we successfully expressed yeast codon-optimized full-length versions of tagged and untagged wild-type kAE1 and demonstrated their partial localization at the yeast plasma membrane (PM). Finally, pH and chloride measurements further suggest biological activity of full-length kAE1, emphasizing the potential of S. cerevisiae as a model system for studying trafficking, activity, and/or degradation of mammalian ion channels and transporters such as kAE1 in the future. IMPORTANCE Distal renal tubular acidosis (dRTA) is a common kidney dysfunction characterized by impaired acid secretion via urine. Previous studies revealed that α-intercalated cells of dRTA patients express mutated forms of human kidney anion exchanger 1 (kAE1) which result in inefficient plasma membrane targeting or diminished expression levels of kAE1. However, the precise dRTA-causing processes are inadequately understood, and alternative model systems are helpful tools to address kAE1-related questions in a fast and inexpensive way. In contrast to a previous study, we successfully expressed full-length kAE1 in Saccharomyces cerevisiae. Using advanced microscopy techniques as well as different biochemical and functionality assays, plasma membrane localization and biological activity were confirmed for the heterologously expressed anion transporter. These findings represent first important steps to use the potential of yeast as a model organism for studying trafficking, activity, and degradation of kAE1 and its mutant variants in the future.
format	article
author	Hasib A. M. Sarder Xiaobing Li Charlotta Funaya Emmanuelle Cordat Manfred J. Schmitt Björn Becker
author_facet	Hasib A. M. Sarder Xiaobing Li Charlotta Funaya Emmanuelle Cordat Manfred J. Schmitt Björn Becker
author_sort	Hasib A. M. Sarder
title	<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1
title_short	<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1
title_full	<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1
title_fullStr	<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1
title_full_unstemmed	<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1
title_sort	<named-content content-type="genus-species">saccharomyces cerevisiae</named-content>: first steps to a suitable model system to study the function and intracellular transport of human kidney anion exchanger 1
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/545a465098ae4202b8c81d0e6afeeb9f
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<named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>: First Steps to a Suitable Model System To Study the Function and Intracellular Transport of Human Kidney Anion Exchanger 1

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