Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys

PCR amplification and sequencing of marker genes are a low-cost technique for monitoring prokaryotic and eukaryotic microbial communities across space and time but will work optimally only if environmental organisms match PCR primer sequences exactly. In this study, we evaluated how well primers ma...

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Autores principales:	Jesse McNichol, Paul M. Berube, Steven J. Biller, Jed A. Fuhrman
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/545ab02f97a3485c8626124d01536202
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spelling	oai:doaj.org-article:545ab02f97a3485c8626124d015362022021-12-02T15:16:36ZEvaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys2379-507710.1128/mSystems.00565-21https://doaj.org/article/545ab02f97a3485c8626124d015362022021-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00565-21https://doaj.org/toc/2379-5077 PCR amplification and sequencing of marker genes are a low-cost technique for monitoring prokaryotic and eukaryotic microbial communities across space and time but will work optimally only if environmental organisms match PCR primer sequences exactly. In this study, we evaluated how well primers match globally distributed short-read oceanic metagenomes.Jesse McNicholPaul M. BerubeSteven J. BillerJed A. FuhrmanAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jesse McNichol Paul M. Berube Steven J. Biller Jed A. Fuhrman Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
description	PCR amplification and sequencing of marker genes are a low-cost technique for monitoring prokaryotic and eukaryotic microbial communities across space and time but will work optimally only if environmental organisms match PCR primer sequences exactly. In this study, we evaluated how well primers match globally distributed short-read oceanic metagenomes.
format	article
author	Jesse McNichol Paul M. Berube Steven J. Biller Jed A. Fuhrman
author_facet	Jesse McNichol Paul M. Berube Steven J. Biller Jed A. Fuhrman
author_sort	Jesse McNichol
title	Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
title_short	Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
title_full	Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
title_fullStr	Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
title_full_unstemmed	Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys
title_sort	evaluating and improving small subunit rrna pcr primer coverage for bacteria, archaea, and eukaryotes using metagenomes from global ocean surveys
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/545ab02f97a3485c8626124d01536202
work_keys_str_mv	AT jessemcnichol evaluatingandimprovingsmallsubunitrrnapcrprimercoverageforbacteriaarchaeaandeukaryotesusingmetagenomesfromglobaloceansurveys AT paulmberube evaluatingandimprovingsmallsubunitrrnapcrprimercoverageforbacteriaarchaeaandeukaryotesusingmetagenomesfromglobaloceansurveys AT stevenjbiller evaluatingandimprovingsmallsubunitrrnapcrprimercoverageforbacteriaarchaeaandeukaryotesusingmetagenomesfromglobaloceansurveys AT jedafuhrman evaluatingandimprovingsmallsubunitrrnapcrprimercoverageforbacteriaarchaeaandeukaryotesusingmetagenomesfromglobaloceansurveys
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Evaluating and Improving Small Subunit rRNA PCR Primer Coverage for Bacteria, Archaea, and Eukaryotes Using Metagenomes from Global Ocean Surveys

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