Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study
We previously reported that sputum induction was more sensitive than throat swabs for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two convalescent coronavirus disease 2019 (COVID-19) patients; however, the value and safety of induced sputum testing require furthe...
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2020
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oai:doaj.org-article:545c89cb2c104ac1a538ebdd8e8b32202021-11-17T14:21:59ZReliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study2150-55942150-560810.1080/21505594.2020.1831342https://doaj.org/article/545c89cb2c104ac1a538ebdd8e8b32202020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1831342https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608We previously reported that sputum induction was more sensitive than throat swabs for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two convalescent coronavirus disease 2019 (COVID-19) patients; however, the value and safety of induced sputum testing require further study. We conducted a prospective multi-center cross-sectional study to compare induced sputum to throat swabs for SARS-CoV-2 detection. Confirmed COVID-19 patients from six hospitals in six cities across China who received one or more negative RT-PCR result for SARS-CoV-2 were enrolled, and paired specimens (induced sputum and throat swabs; 56 cases) were assayed. In three paired samples, both the induced sputum and throat swabs were positive for SARS-CoV-2. The positive rate for induced sputum was significantly higher than for throat swabs both overall (28.6% vs 5.4%, respectively; p < 0.01). Patients were divided according to time span from onset of illness to sample collection into the more-than-30-day (n = 26) and less-than-30-day (n = 30) groups. The positive rate for induced sputum was also significantly higher than for throat swabs in the less-than-30-day group (53.3% vs 10.0%, respectively; p < 0.001). For the more-than-30-day group, all paired samples were negative for SARS-CoV-2. Blood oxygen saturation, respiratory rate, and heart rate remained stable during sputum induction and no staff were infected. Because induced sputum is more reliable and has a lower false-negative rate than throat swabs, we believe induced sputum is more useful for the confirmation of COVID-19 and is safer as a criterion for release from quarantine.Tianwen LaiFangfei XiangJianfeng ZengYingzi HuangLiping JiaHui ChenJiayuan WuJianfeng XieShuna LiuWei DengWeiqiang ZhengYang HuangQinfu ZhangQingfeng LuoFan MoLieming LongWuying ZhangWenna ChenHuanqin HanTaylor & Francis Grouparticlecovid-19reliabilityinduced sputumthroat swabsars-cov-2Infectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1394-1401 (2020) |
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covid-19 reliability induced sputum throat swab sars-cov-2 Infectious and parasitic diseases RC109-216 |
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covid-19 reliability induced sputum throat swab sars-cov-2 Infectious and parasitic diseases RC109-216 Tianwen Lai Fangfei Xiang Jianfeng Zeng Yingzi Huang Liping Jia Hui Chen Jiayuan Wu Jianfeng Xie Shuna Liu Wei Deng Weiqiang Zheng Yang Huang Qinfu Zhang Qingfeng Luo Fan Mo Lieming Long Wuying Zhang Wenna Chen Huanqin Han Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study |
| description |
We previously reported that sputum induction was more sensitive than throat swabs for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two convalescent coronavirus disease 2019 (COVID-19) patients; however, the value and safety of induced sputum testing require further study. We conducted a prospective multi-center cross-sectional study to compare induced sputum to throat swabs for SARS-CoV-2 detection. Confirmed COVID-19 patients from six hospitals in six cities across China who received one or more negative RT-PCR result for SARS-CoV-2 were enrolled, and paired specimens (induced sputum and throat swabs; 56 cases) were assayed. In three paired samples, both the induced sputum and throat swabs were positive for SARS-CoV-2. The positive rate for induced sputum was significantly higher than for throat swabs both overall (28.6% vs 5.4%, respectively; p < 0.01). Patients were divided according to time span from onset of illness to sample collection into the more-than-30-day (n = 26) and less-than-30-day (n = 30) groups. The positive rate for induced sputum was also significantly higher than for throat swabs in the less-than-30-day group (53.3% vs 10.0%, respectively; p < 0.001). For the more-than-30-day group, all paired samples were negative for SARS-CoV-2. Blood oxygen saturation, respiratory rate, and heart rate remained stable during sputum induction and no staff were infected. Because induced sputum is more reliable and has a lower false-negative rate than throat swabs, we believe induced sputum is more useful for the confirmation of COVID-19 and is safer as a criterion for release from quarantine. |
| format |
article |
| author |
Tianwen Lai Fangfei Xiang Jianfeng Zeng Yingzi Huang Liping Jia Hui Chen Jiayuan Wu Jianfeng Xie Shuna Liu Wei Deng Weiqiang Zheng Yang Huang Qinfu Zhang Qingfeng Luo Fan Mo Lieming Long Wuying Zhang Wenna Chen Huanqin Han |
| author_facet |
Tianwen Lai Fangfei Xiang Jianfeng Zeng Yingzi Huang Liping Jia Hui Chen Jiayuan Wu Jianfeng Xie Shuna Liu Wei Deng Weiqiang Zheng Yang Huang Qinfu Zhang Qingfeng Luo Fan Mo Lieming Long Wuying Zhang Wenna Chen Huanqin Han |
| author_sort |
Tianwen Lai |
| title |
Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study |
| title_short |
Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study |
| title_full |
Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study |
| title_fullStr |
Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study |
| title_full_unstemmed |
Reliability of induced sputum test is greater than that of throat swab test for detecting SARS-CoV-2 in patients with COVID-19: A multi-center cross-sectional study |
| title_sort |
reliability of induced sputum test is greater than that of throat swab test for detecting sars-cov-2 in patients with covid-19: a multi-center cross-sectional study |
| publisher |
Taylor & Francis Group |
| publishDate |
2020 |
| url |
https://doaj.org/article/545c89cb2c104ac1a538ebdd8e8b3220 |
| work_keys_str_mv |
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