Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.

Energy-rich diets can challenge metabolic and protective functions of the rumen epithelial cells, but the underlying factors are unclear. This study sought to evaluate proteomic changes of the rumen epithelium in goats fed a low, medium, or high energy diet. Expression of protein changes were compar...

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Autores principales:	Manfred Hollmann, Ingrid Miller, Karin Hummel, Sonja Sabitzer, Barbara U Metzler-Zebeli, Ebrahim Razzazi-Fazeli, Qendrim Zebeli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5460519751c842bb9919aa76e328649f
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spelling	oai:doaj.org-article:5460519751c842bb9919aa76e328649f2021-11-18T08:42:56ZDownregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.1932-620310.1371/journal.pone.0081602https://doaj.org/article/5460519751c842bb9919aa76e328649f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349094/?tool=EBIhttps://doaj.org/toc/1932-6203Energy-rich diets can challenge metabolic and protective functions of the rumen epithelial cells, but the underlying factors are unclear. This study sought to evaluate proteomic changes of the rumen epithelium in goats fed a low, medium, or high energy diet. Expression of protein changes were compared by two-dimensional differential gel electrophoresis followed by protein identification with matrix assisted laser desorption ionisation tandem time-of-flight mass spectrometry. Of about 2,000 spots commonly detected in all gels, 64 spots were significantly regulated, which were traced back to 24 unique proteins. Interestingly, the expression profiles of several chaperone proteins with important cellular protective functions such as heat shock cognate 71 kDa protein, peroxiredoxin-6, serpin H1, protein disulfide-isomerase, and selenium-binding protein were collectively downregulated in response to high dietary energy supply. Similar regulation patterns were obtained for some other proteins involved in transport or metabolic functions. In contrast, metabolic enzymes like retinal dehydrogenase 1 and ATP synthase subunit beta, mitochondrial precursor were upregulated in response to high energy diet. Lower expressions of chaperone proteins in the rumen epithelial cells in response to high energy supply may suggest that these cells were less protected against the potentially harmful rumen toxic compounds, which might have consequences for rumen and systemic health. Our findings also suggest that energy-rich diets and the resulting acidotic insult may render rumen epithelial cells more vulnerable to cellular damage by attenuating their cell defense system, hence facilitating the impairment of rumen barrier function, typically observed in energy-rich fed ruminants.Manfred HollmannIngrid MillerKarin HummelSonja SabitzerBarbara U Metzler-ZebeliEbrahim Razzazi-FazeliQendrim ZebeliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81602 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manfred Hollmann Ingrid Miller Karin Hummel Sonja Sabitzer Barbara U Metzler-Zebeli Ebrahim Razzazi-Fazeli Qendrim Zebeli Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
description	Energy-rich diets can challenge metabolic and protective functions of the rumen epithelial cells, but the underlying factors are unclear. This study sought to evaluate proteomic changes of the rumen epithelium in goats fed a low, medium, or high energy diet. Expression of protein changes were compared by two-dimensional differential gel electrophoresis followed by protein identification with matrix assisted laser desorption ionisation tandem time-of-flight mass spectrometry. Of about 2,000 spots commonly detected in all gels, 64 spots were significantly regulated, which were traced back to 24 unique proteins. Interestingly, the expression profiles of several chaperone proteins with important cellular protective functions such as heat shock cognate 71 kDa protein, peroxiredoxin-6, serpin H1, protein disulfide-isomerase, and selenium-binding protein were collectively downregulated in response to high dietary energy supply. Similar regulation patterns were obtained for some other proteins involved in transport or metabolic functions. In contrast, metabolic enzymes like retinal dehydrogenase 1 and ATP synthase subunit beta, mitochondrial precursor were upregulated in response to high energy diet. Lower expressions of chaperone proteins in the rumen epithelial cells in response to high energy supply may suggest that these cells were less protected against the potentially harmful rumen toxic compounds, which might have consequences for rumen and systemic health. Our findings also suggest that energy-rich diets and the resulting acidotic insult may render rumen epithelial cells more vulnerable to cellular damage by attenuating their cell defense system, hence facilitating the impairment of rumen barrier function, typically observed in energy-rich fed ruminants.
format	article
author	Manfred Hollmann Ingrid Miller Karin Hummel Sonja Sabitzer Barbara U Metzler-Zebeli Ebrahim Razzazi-Fazeli Qendrim Zebeli
author_facet	Manfred Hollmann Ingrid Miller Karin Hummel Sonja Sabitzer Barbara U Metzler-Zebeli Ebrahim Razzazi-Fazeli Qendrim Zebeli
author_sort	Manfred Hollmann
title	Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
title_short	Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
title_full	Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
title_fullStr	Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
title_full_unstemmed	Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
title_sort	downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5460519751c842bb9919aa76e328649f
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Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.

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