Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation

Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation

Sun Young Park,1 Seon Yeong Chae,1,2 Jin Oh Park,2 Kyu Jin Lee,2 Geuntae Park1,2 1Bio-IT Fusion Technology Research Institute, 2Department of Nanofusion Technology, Graduate School, Pusan National University, Busan, Republic of Korea Abstract: Recently, environment-friendly synthesis of gold nanop...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Park SY, Chae SY, Park JO, Lee KJ, Park G
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
gold nanoparticle
Kalopanacis Cortex
oxygen–glucose deprivation
neuroprotection
NRF2
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/5460acba2cef4b259117c2a8b8718800
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:5460acba2cef4b259117c2a8b8718800
record_format dspace
spelling oai:doaj.org-article:5460acba2cef4b259117c2a8b87188002021-12-02T03:58:32ZKalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation1178-2013https://doaj.org/article/5460acba2cef4b259117c2a8b87188002017-06-01T00:00:00Zhttps://www.dovepress.com/kalopanacis-cortex-extract-capped-gold-nanoparticles-activate-nrf2-sig-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sun Young Park,1 Seon Yeong Chae,1,2 Jin Oh Park,2 Kyu Jin Lee,2 Geuntae Park1,2 1Bio-IT Fusion Technology Research Institute, 2Department of Nanofusion Technology, Graduate School, Pusan National University, Busan, Republic of Korea Abstract: Recently, environment-friendly synthesis of gold nanoparticles (GNPs) has been extensively explored by biologists and chemists. However, significant research is still required to determine whether “eco-friendly” GNPs are beneficial to human health and to elucidate the molecular mechanisms of their effects on human cells. We used human neuronal SH-SY5Y cells to show that treatment with Kalopanacis Cortex extract-capped GNPs (KC-GNs), prepared via an eco-friendly, fast, one-pot synthetic route, protected neuronal cells against oxygen–glucose deprivation/reoxygenation (OGD/R)-induced damage. To prepare GNPs, Kalopanacis Cortex was used without any chemical reducing and stabilizing agents. Ultraviolet–visible spectroscopy showed maximum absorbance at 526 nm owing to KC-GN surface plasmon resonance. Hydrodynamic size (54.02±2.19 nm) and zeta potential (-20.3±0.04 mV) were determined by dynamic light scattering. The average diameter (41.07±3.05 nm) was determined by high-resolution transmission electron microscopy. Energy-dispersive X-ray diffraction spectroscopy and X-ray diffraction confirmed the presence of assembled GNPs. Fourier transform infrared analysis suggested that functional groups such as O–H, C–C, and C–N participated in KC-GN formation. Cell viability assays indicated that KC-GNs restored the viability of OGD/R-treated SH-SY5Y cells. Flow cytometry demonstrated that KC-GNs inhibited the OGD/R-induced reactive oxygen species production and mitochondrial membrane potential disruption. KC-GNs also inhibited the apoptosis of OGD/R-exposed cells. Western blot analysis indicated that the OGD/R-induced cellular apoptosis and simultaneous increases in the expression of cleaved caspase-3, p53, p21, and B-cell lymphoma 2-associated X protein were reversed by KC-GNs. The KC-GN-mediated protection against OGD/R-induced neurotoxicity was diminished by NRF2 and heme oxygenase-1 gene knockdowns. Collectively, these results suggested that KC-GNs exerted strong neuroprotective effects on human neuronal cells, which might be attributed to the attenuation of OGD/R-induced neuronal cell injury through the NRF2 signaling pathway. Keywords: gold nanoparticle, Kalopanacis Cortex, oxygen–glucose deprivation, neuroprotection, NRF2 Park SYChae SYPark JOLee KJPark GDove Medical Pressarticlegold nanoparticleKalopanacis Cortexoxygen–glucose deprivationneuroprotectionNRF2Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4563-4578 (2017)
institution DOAJ
collection DOAJ
language EN
topic gold nanoparticle
Kalopanacis Cortex
oxygen–glucose deprivation
neuroprotection
NRF2
Medicine (General)
R5-920
spellingShingle gold nanoparticle
Kalopanacis Cortex
oxygen–glucose deprivation
neuroprotection
NRF2
Medicine (General)
R5-920
Park SY
Chae SY
Park JO
Lee KJ
Park G
Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation
description Sun Young Park,1 Seon Yeong Chae,1,2 Jin Oh Park,2 Kyu Jin Lee,2 Geuntae Park1,2 1Bio-IT Fusion Technology Research Institute, 2Department of Nanofusion Technology, Graduate School, Pusan National University, Busan, Republic of Korea Abstract: Recently, environment-friendly synthesis of gold nanoparticles (GNPs) has been extensively explored by biologists and chemists. However, significant research is still required to determine whether “eco-friendly” GNPs are beneficial to human health and to elucidate the molecular mechanisms of their effects on human cells. We used human neuronal SH-SY5Y cells to show that treatment with Kalopanacis Cortex extract-capped GNPs (KC-GNs), prepared via an eco-friendly, fast, one-pot synthetic route, protected neuronal cells against oxygen–glucose deprivation/reoxygenation (OGD/R)-induced damage. To prepare GNPs, Kalopanacis Cortex was used without any chemical reducing and stabilizing agents. Ultraviolet–visible spectroscopy showed maximum absorbance at 526 nm owing to KC-GN surface plasmon resonance. Hydrodynamic size (54.02±2.19 nm) and zeta potential (-20.3±0.04 mV) were determined by dynamic light scattering. The average diameter (41.07±3.05 nm) was determined by high-resolution transmission electron microscopy. Energy-dispersive X-ray diffraction spectroscopy and X-ray diffraction confirmed the presence of assembled GNPs. Fourier transform infrared analysis suggested that functional groups such as O–H, C–C, and C–N participated in KC-GN formation. Cell viability assays indicated that KC-GNs restored the viability of OGD/R-treated SH-SY5Y cells. Flow cytometry demonstrated that KC-GNs inhibited the OGD/R-induced reactive oxygen species production and mitochondrial membrane potential disruption. KC-GNs also inhibited the apoptosis of OGD/R-exposed cells. Western blot analysis indicated that the OGD/R-induced cellular apoptosis and simultaneous increases in the expression of cleaved caspase-3, p53, p21, and B-cell lymphoma 2-associated X protein were reversed by KC-GNs. The KC-GN-mediated protection against OGD/R-induced neurotoxicity was diminished by NRF2 and heme oxygenase-1 gene knockdowns. Collectively, these results suggested that KC-GNs exerted strong neuroprotective effects on human neuronal cells, which might be attributed to the attenuation of OGD/R-induced neuronal cell injury through the NRF2 signaling pathway. Keywords: gold nanoparticle, Kalopanacis Cortex, oxygen–glucose deprivation, neuroprotection, NRF2 
format article
author Park SY
Chae SY
Park JO
Lee KJ
Park G
author_facet Park SY
Chae SY
Park JO
Lee KJ
Park G
author_sort Park SY
title Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation
title_short Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation
title_full Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation
title_fullStr Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation
title_full_unstemmed Kalopanacis Cortex extract-capped gold nanoparticles activate NRF2 signaling and ameliorate damage in human neuronal SH-SY5Y cells exposed to oxygen–glucose deprivation and reoxygenation
title_sort kalopanacis cortex extract-capped gold nanoparticles activate nrf2 signaling and ameliorate damage in human neuronal sh-sy5y cells exposed to oxygen–glucose deprivation and reoxygenation
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/5460acba2cef4b259117c2a8b8718800
work_keys_str_mv AT parksy kalopanaciscortexextractcappedgoldnanoparticlesactivatenrf2signalingandamelioratedamageinhumanneuronalshsy5ycellsexposedtooxygenndashglucosedeprivationandreoxygenation
AT chaesy kalopanaciscortexextractcappedgoldnanoparticlesactivatenrf2signalingandamelioratedamageinhumanneuronalshsy5ycellsexposedtooxygenndashglucosedeprivationandreoxygenation
AT parkjo kalopanaciscortexextractcappedgoldnanoparticlesactivatenrf2signalingandamelioratedamageinhumanneuronalshsy5ycellsexposedtooxygenndashglucosedeprivationandreoxygenation
AT leekj kalopanaciscortexextractcappedgoldnanoparticlesactivatenrf2signalingandamelioratedamageinhumanneuronalshsy5ycellsexposedtooxygenndashglucosedeprivationandreoxygenation
AT parkg kalopanaciscortexextractcappedgoldnanoparticlesactivatenrf2signalingandamelioratedamageinhumanneuronalshsy5ycellsexposedtooxygenndashglucosedeprivationandreoxygenation
_version_ 1718401486342324224

Ejemplares similares