Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers

Abstract. Husnudin UB, Daryono BS, Purnomo. 2019. Genetic variability of Indonesian Eggplant (Solanum melongena) based on ISSR markers. Biodiversitas 20: 3049-3055. Eggplant (Solanum melongena L.) is one of important vegetable in Indonesia and this country has a variety of eggplant germplasm, wherea...

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Autores principales:	U.B. HUSNUDIN, B.S. DARYONO, Purnomo Purnomo
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	genetic, indonesia, phenetic relationship, solanum melongena, variability Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5465357542df446090d31269c0946fc9
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spelling	oai:doaj.org-article:5465357542df446090d31269c0946fc92021-11-21T21:54:57ZGenetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers1412-033X2085-472210.13057/biodiv/d201038https://doaj.org/article/5465357542df446090d31269c0946fc92019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4233https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Husnudin UB, Daryono BS, Purnomo. 2019. Genetic variability of Indonesian Eggplant (Solanum melongena) based on ISSR markers. Biodiversitas 20: 3049-3055. Eggplant (Solanum melongena L.) is one of important vegetable in Indonesia and this country has a variety of eggplant germplasm, whereas molecular data of Indonesian eggplant is limited. This study describes an analysis of genetic variation based on ISSR markers to determine the diversity and phenetic relationship of Indonesian eggplants. 23 samples were collected from the Indonesian Center for Agricultural Biotechnology Research and Development (ICABIOGRAD) and some commercial cultivars. Data were analyzed by ISSR analysis using UBC 809, UBC 880, UBC 888, UBC 892 and UBC 895 primers. Similarity index was counted by Jaccard Coefficient formula based on molecular scoring. Cluster analysis was conducted by Unweighted Pair Group Methods using Arithmetic averages (UPGMA) method to create a dendrogram with Multivariate Statistical Program (MVSP) v.3.1 software. The results showed that ISSR markers were effective in the estimation of the genetic variability of eggplant accessions characterized by different level of polymorphism. Five ISSR primers generated 40 polymorphic bands (64.5% of the total). The dendrogram divided 23 eggplant accessions into 2 main clusters and one accession ("terong jawa"/K18) located outside from the main cluster. Results suggested that all accessions were grouped randomly into some clusters not in accordance with the locations of sample collection.U.B. HUSNUDINB.S. DARYONOPurnomo PurnomoMBI & UNS Soloarticlegenetic, indonesia, phenetic relationship, solanum melongena, variabilityBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic, indonesia, phenetic relationship, solanum melongena, variability Biology (General) QH301-705.5
spellingShingle	genetic, indonesia, phenetic relationship, solanum melongena, variability Biology (General) QH301-705.5 U.B. HUSNUDIN B.S. DARYONO Purnomo Purnomo Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers
description	Abstract. Husnudin UB, Daryono BS, Purnomo. 2019. Genetic variability of Indonesian Eggplant (Solanum melongena) based on ISSR markers. Biodiversitas 20: 3049-3055. Eggplant (Solanum melongena L.) is one of important vegetable in Indonesia and this country has a variety of eggplant germplasm, whereas molecular data of Indonesian eggplant is limited. This study describes an analysis of genetic variation based on ISSR markers to determine the diversity and phenetic relationship of Indonesian eggplants. 23 samples were collected from the Indonesian Center for Agricultural Biotechnology Research and Development (ICABIOGRAD) and some commercial cultivars. Data were analyzed by ISSR analysis using UBC 809, UBC 880, UBC 888, UBC 892 and UBC 895 primers. Similarity index was counted by Jaccard Coefficient formula based on molecular scoring. Cluster analysis was conducted by Unweighted Pair Group Methods using Arithmetic averages (UPGMA) method to create a dendrogram with Multivariate Statistical Program (MVSP) v.3.1 software. The results showed that ISSR markers were effective in the estimation of the genetic variability of eggplant accessions characterized by different level of polymorphism. Five ISSR primers generated 40 polymorphic bands (64.5% of the total). The dendrogram divided 23 eggplant accessions into 2 main clusters and one accession ("terong jawa"/K18) located outside from the main cluster. Results suggested that all accessions were grouped randomly into some clusters not in accordance with the locations of sample collection.
format	article
author	U.B. HUSNUDIN B.S. DARYONO Purnomo Purnomo
author_facet	U.B. HUSNUDIN B.S. DARYONO Purnomo Purnomo
author_sort	U.B. HUSNUDIN
title	Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers
title_short	Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers
title_full	Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers
title_fullStr	Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers
title_full_unstemmed	Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers
title_sort	genetic variability of indonesian eggplant (solanum melongena) based on issr markers
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/5465357542df446090d31269c0946fc9
work_keys_str_mv	AT ubhusnudin geneticvariabilityofindonesianeggplantsolanummelongenabasedonissrmarkers AT bsdaryono geneticvariabilityofindonesianeggplantsolanummelongenabasedonissrmarkers AT purnomopurnomo geneticvariabilityofindonesianeggplantsolanummelongenabasedonissrmarkers
_version_	1718418642241060864

Genetic variability of Indonesian eggplant (Solanum melongena) based on ISSR markers

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