Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

Gold nanoparticles (AuNP) capped with biocompatible layers have functional optical, chemical, and biological properties as theranostic agents in biomedicine. The ferritin protein containing in situ synthesized AuNPs has been successfully used as an effective and completely biocompatible nanocarrier...

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Autores principales:	Italo Moglia, Margarita Santiago, Simon Guerrero, Mónica Soler, Alvaro Olivera-Nappa, Marcelo J. Kogan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ferritin gold nanoparticles cellular internalization cellular viability theranostics Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/5466e8c2f934472897dd46e7f682668a
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spelling	oai:doaj.org-article:5466e8c2f934472897dd46e7f682668a2021-11-25T18:42:27ZEnhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles10.3390/pharmaceutics131119661999-4923https://doaj.org/article/5466e8c2f934472897dd46e7f682668a2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1966https://doaj.org/toc/1999-4923Gold nanoparticles (AuNP) capped with biocompatible layers have functional optical, chemical, and biological properties as theranostic agents in biomedicine. The ferritin protein containing in situ synthesized AuNPs has been successfully used as an effective and completely biocompatible nanocarrier for AuNPs in human cell lines and animal experiments in vivo. Ferritin can be uptaken by different cell types through receptor-mediated endocytosis. Despite these advantages, few efforts have been made to evaluate the toxicity and cellular internalization of AuNP-containing ferritin nanocages. In this work, we study the potential of human heavy-chain (H) and light-chain (L) ferritin homopolymers as nanoreactors to synthesize AuNPs and their cytotoxicity and cellular uptake in different cell lines. The results show very low toxicity of ferritin-encapsulated AuNPs on different human cell lines and demonstrate that efficient cellular ferritin uptake depends on the specific H or L protein chains forming the ferritin protein cage and the presence or absence of metallic cargo. Cargo-devoid apoferritin is poorly internalized in all cell lines, and the highest ferritin uptake was achieved with AuNP-loaded H-ferritin homopolymers in transferrin-receptor-rich cell lines, showing more than seven times more uptake than apoferritin.Italo MogliaMargarita SantiagoSimon GuerreroMónica SolerAlvaro Olivera-NappaMarcelo J. KoganMDPI AGarticleferritingold nanoparticlescellular internalizationcellular viabilitytheranosticsPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1966, p 1966 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ferritin gold nanoparticles cellular internalization cellular viability theranostics Pharmacy and materia medica RS1-441
spellingShingle	ferritin gold nanoparticles cellular internalization cellular viability theranostics Pharmacy and materia medica RS1-441 Italo Moglia Margarita Santiago Simon Guerrero Mónica Soler Alvaro Olivera-Nappa Marcelo J. Kogan Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles
description	Gold nanoparticles (AuNP) capped with biocompatible layers have functional optical, chemical, and biological properties as theranostic agents in biomedicine. The ferritin protein containing in situ synthesized AuNPs has been successfully used as an effective and completely biocompatible nanocarrier for AuNPs in human cell lines and animal experiments in vivo. Ferritin can be uptaken by different cell types through receptor-mediated endocytosis. Despite these advantages, few efforts have been made to evaluate the toxicity and cellular internalization of AuNP-containing ferritin nanocages. In this work, we study the potential of human heavy-chain (H) and light-chain (L) ferritin homopolymers as nanoreactors to synthesize AuNPs and their cytotoxicity and cellular uptake in different cell lines. The results show very low toxicity of ferritin-encapsulated AuNPs on different human cell lines and demonstrate that efficient cellular ferritin uptake depends on the specific H or L protein chains forming the ferritin protein cage and the presence or absence of metallic cargo. Cargo-devoid apoferritin is poorly internalized in all cell lines, and the highest ferritin uptake was achieved with AuNP-loaded H-ferritin homopolymers in transferrin-receptor-rich cell lines, showing more than seven times more uptake than apoferritin.
format	article
author	Italo Moglia Margarita Santiago Simon Guerrero Mónica Soler Alvaro Olivera-Nappa Marcelo J. Kogan
author_facet	Italo Moglia Margarita Santiago Simon Guerrero Mónica Soler Alvaro Olivera-Nappa Marcelo J. Kogan
author_sort	Italo Moglia
title	Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles
title_short	Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles
title_full	Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles
title_fullStr	Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles
title_full_unstemmed	Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles
title_sort	enhanced cellular uptake of h-chain human ferritin containing gold nanoparticles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5466e8c2f934472897dd46e7f682668a
work_keys_str_mv	AT italomoglia enhancedcellularuptakeofhchainhumanferritincontaininggoldnanoparticles AT margaritasantiago enhancedcellularuptakeofhchainhumanferritincontaininggoldnanoparticles AT simonguerrero enhancedcellularuptakeofhchainhumanferritincontaininggoldnanoparticles AT monicasoler enhancedcellularuptakeofhchainhumanferritincontaininggoldnanoparticles AT alvarooliveranappa enhancedcellularuptakeofhchainhumanferritincontaininggoldnanoparticles AT marcelojkogan enhancedcellularuptakeofhchainhumanferritincontaininggoldnanoparticles
_version_	1718410771598147584

Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

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