Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.

<h4>Background</h4>Bunyamwera(BUNV) and Ngari (NGIV) viruses are arboviruses of medical importance globally, the viruses are endemic in Africa, Aedes(Ae) aegypti and Anopheles(An) gambiae mosquitoes are currently competent vectors for BUNV and NGIV respectively. Both viruses have been is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	James Mutisya, Michael Kahato, Francis Mulwa, Solomon Langat, Edith Chepkorir, Samuel Arum, David Tchouassi, Rosemary Sang, Joel Lutomiah
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54689f0de8ae45c1ab2fa3ae6f866187
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:54689f0de8ae45c1ab2fa3ae6f866187
record_format	dspace
spelling	oai:doaj.org-article:54689f0de8ae45c1ab2fa3ae6f8661872021-12-02T20:09:44ZEvaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.1932-620310.1371/journal.pone.0253955https://doaj.org/article/54689f0de8ae45c1ab2fa3ae6f8661872021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253955https://doaj.org/toc/1932-6203<h4>Background</h4>Bunyamwera(BUNV) and Ngari (NGIV) viruses are arboviruses of medical importance globally, the viruses are endemic in Africa, Aedes(Ae) aegypti and Anopheles(An) gambiae mosquitoes are currently competent vectors for BUNV and NGIV respectively. Both viruses have been isolated from humans and mosquitoes in various ecologies of Kenya. Understanding the risk patterns and spread of the viruses necessitate studies of vector competence in local vector population of Ae. simpsoni sl which is abundant in the coastal region. This study sought to assess the ability of Ae. Simpsoni sl mosquitoes abundant at the Coast of Kenya to transmit these viruses in experimental laboratory experiments.<h4>Methods</h4>Field collected larvae/pupae of Ae. Simpsoni sl mosquitoes from Rabai, Kilifi County, were reared to adults, the first filial generation (F0) females' mosquitoes were orally exposed to infectious blood meal with isolates of the viruses using the hemotek membrane feeder. The exposed mosquitoes were incubated under insectary conditions and sampled on day 7, 14 and 21days post infection to determine susceptibility to the virus infection using plaque assay.<h4>Results</h4>A total of 379 (Bunyamwera virus 255 and Ngari virus 124) Ae. simpsoni sl were orally exposed to infectious blood meal. Overall, the infection rate (IR) for BUNV and NGIV were 2.7 and 0.9% respectively. Dissemination occurred in 5 out 7 mosquitoes with mid-gut infection for Bunyamwera virus and 1 out of 2 mosquitoes with mid-gut infection for Ngari virus. Further, the transmission was observed in 1 out of 5 mosquitoes that had disseminated infection and no transmission was observed for Ngari virus in all days post infection (dpi).<h4>Conclusion</h4>Our study shows that Ae. simpsoni sl. is a laboratory competent vector for Bunyamwera virus since it was able to transmit the virus through capillary feeding while NGIV infection was restricted to midgut infection and disseminated infection, these finding adds information on the epidemiology of the viruses and vector control plan.James MutisyaMichael KahatoFrancis MulwaSolomon LangatEdith ChepkorirSamuel ArumDavid TchouassiRosemary SangJoel LutomiahPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0253955 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q James Mutisya Michael Kahato Francis Mulwa Solomon Langat Edith Chepkorir Samuel Arum David Tchouassi Rosemary Sang Joel Lutomiah Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.
description	<h4>Background</h4>Bunyamwera(BUNV) and Ngari (NGIV) viruses are arboviruses of medical importance globally, the viruses are endemic in Africa, Aedes(Ae) aegypti and Anopheles(An) gambiae mosquitoes are currently competent vectors for BUNV and NGIV respectively. Both viruses have been isolated from humans and mosquitoes in various ecologies of Kenya. Understanding the risk patterns and spread of the viruses necessitate studies of vector competence in local vector population of Ae. simpsoni sl which is abundant in the coastal region. This study sought to assess the ability of Ae. Simpsoni sl mosquitoes abundant at the Coast of Kenya to transmit these viruses in experimental laboratory experiments.<h4>Methods</h4>Field collected larvae/pupae of Ae. Simpsoni sl mosquitoes from Rabai, Kilifi County, were reared to adults, the first filial generation (F0) females' mosquitoes were orally exposed to infectious blood meal with isolates of the viruses using the hemotek membrane feeder. The exposed mosquitoes were incubated under insectary conditions and sampled on day 7, 14 and 21days post infection to determine susceptibility to the virus infection using plaque assay.<h4>Results</h4>A total of 379 (Bunyamwera virus 255 and Ngari virus 124) Ae. simpsoni sl were orally exposed to infectious blood meal. Overall, the infection rate (IR) for BUNV and NGIV were 2.7 and 0.9% respectively. Dissemination occurred in 5 out 7 mosquitoes with mid-gut infection for Bunyamwera virus and 1 out of 2 mosquitoes with mid-gut infection for Ngari virus. Further, the transmission was observed in 1 out of 5 mosquitoes that had disseminated infection and no transmission was observed for Ngari virus in all days post infection (dpi).<h4>Conclusion</h4>Our study shows that Ae. simpsoni sl. is a laboratory competent vector for Bunyamwera virus since it was able to transmit the virus through capillary feeding while NGIV infection was restricted to midgut infection and disseminated infection, these finding adds information on the epidemiology of the viruses and vector control plan.
format	article
author	James Mutisya Michael Kahato Francis Mulwa Solomon Langat Edith Chepkorir Samuel Arum David Tchouassi Rosemary Sang Joel Lutomiah
author_facet	James Mutisya Michael Kahato Francis Mulwa Solomon Langat Edith Chepkorir Samuel Arum David Tchouassi Rosemary Sang Joel Lutomiah
author_sort	James Mutisya
title	Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.
title_short	Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.
title_full	Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.
title_fullStr	Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.
title_full_unstemmed	Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.
title_sort	evaluating the vector competence of aedes simpsoni sl from kenyan coast for ngari and bunyamwera viruses.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/54689f0de8ae45c1ab2fa3ae6f866187
work_keys_str_mv	AT jamesmutisya evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT michaelkahato evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT francismulwa evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT solomonlangat evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT edithchepkorir evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT samuelarum evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT davidtchouassi evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT rosemarysang evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses AT joellutomiah evaluatingthevectorcompetenceofaedessimpsonislfromkenyancoastforngariandbunyamweraviruses
_version_	1718375060959395840

Evaluating the vector competence of Aedes simpsoni sl from Kenyan coast for Ngari and Bunyamwera viruses.

Ejemplares similares