Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses

Abstract We compared the effect of commercial vaginal douching products on Lactobacillus crispatus, L. jensenii, L. gasseri, L. iners, E. coli, and immortalized vaginal epithelial cells (VK2). All studied douching products (vinegar, iodine and baking soda based) induced epithelial cell death, and al...

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Autores principales:	Helai Hesham, Alissa J. Mitchell, Agnes Bergerat, Kristin Hung, Caroline M. Mitchell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54764c31a89a4132a4f7b162825c53e4
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spelling	oai:doaj.org-article:54764c31a89a4132a4f7b162825c53e42021-12-05T12:12:19ZImpact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses10.1038/s41598-021-02426-52045-2322https://doaj.org/article/54764c31a89a4132a4f7b162825c53e42021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02426-5https://doaj.org/toc/2045-2322Abstract We compared the effect of commercial vaginal douching products on Lactobacillus crispatus, L. jensenii, L. gasseri, L. iners, E. coli, and immortalized vaginal epithelial cells (VK2). All studied douching products (vinegar, iodine and baking soda based) induced epithelial cell death, and all inhibited growth of E. coli. Co-culture of vaginal epithelial cells with any of the lactobacilli immediately following exposure to douching products resulted in a trend to less human cell death. However, co-culture of epithelial cells with L. iners was associated with higher production of IL6 and IL8, and lower IL1RA regardless of presence or type of douching solution. Co-culture with L. crispatus or L. jensenii decreased IL6 production in the absence of douches, but increased IL6 production after exposure to vinegar. Douching products may be associated with epithelial disruption and inflammation, and may reduce the anti-inflammatory effects of beneficial lactobacilli.Helai HeshamAlissa J. MitchellAgnes BergeratKristin HungCaroline M. MitchellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Helai Hesham Alissa J. Mitchell Agnes Bergerat Kristin Hung Caroline M. Mitchell Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses
description	Abstract We compared the effect of commercial vaginal douching products on Lactobacillus crispatus, L. jensenii, L. gasseri, L. iners, E. coli, and immortalized vaginal epithelial cells (VK2). All studied douching products (vinegar, iodine and baking soda based) induced epithelial cell death, and all inhibited growth of E. coli. Co-culture of vaginal epithelial cells with any of the lactobacilli immediately following exposure to douching products resulted in a trend to less human cell death. However, co-culture of epithelial cells with L. iners was associated with higher production of IL6 and IL8, and lower IL1RA regardless of presence or type of douching solution. Co-culture with L. crispatus or L. jensenii decreased IL6 production in the absence of douches, but increased IL6 production after exposure to vinegar. Douching products may be associated with epithelial disruption and inflammation, and may reduce the anti-inflammatory effects of beneficial lactobacilli.
format	article
author	Helai Hesham Alissa J. Mitchell Agnes Bergerat Kristin Hung Caroline M. Mitchell
author_facet	Helai Hesham Alissa J. Mitchell Agnes Bergerat Kristin Hung Caroline M. Mitchell
author_sort	Helai Hesham
title	Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses
title_short	Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses
title_full	Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses
title_fullStr	Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses
title_full_unstemmed	Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses
title_sort	impact of vaginal douching products on vaginal lactobacillus, escherichia coli and epithelial immune responses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/54764c31a89a4132a4f7b162825c53e4
work_keys_str_mv	AT helaihesham impactofvaginaldouchingproductsonvaginallactobacillusescherichiacoliandepithelialimmuneresponses AT alissajmitchell impactofvaginaldouchingproductsonvaginallactobacillusescherichiacoliandepithelialimmuneresponses AT agnesbergerat impactofvaginaldouchingproductsonvaginallactobacillusescherichiacoliandepithelialimmuneresponses AT kristinhung impactofvaginaldouchingproductsonvaginallactobacillusescherichiacoliandepithelialimmuneresponses AT carolinemmitchell impactofvaginaldouchingproductsonvaginallactobacillusescherichiacoliandepithelialimmuneresponses
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Impact of vaginal douching products on vaginal Lactobacillus, Escherichia coli and epithelial immune responses

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