Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.

It was found that antibodies (Abs) against myelin basic protein (MBP) are the major components of the antibody response in multiple sclerosis (MS) patients. We have recently shown that IgGs from sera of MS patients are active in the hydrolysis of MBP. However, in literature there are no available da...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Visilii B Doronin, Taisiya A Parkhomenko, Massimiliano Castellazzi, Marina Padroni, Michela Pastore, Valentina N Buneva, Enrico Granieri, Georgy A Nevinsky
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/547691fb82634d8ab56e813ca8fe2d3e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:547691fb82634d8ab56e813ca8fe2d3e
record_format	dspace
spelling	oai:doaj.org-article:547691fb82634d8ab56e813ca8fe2d3e2021-11-25T05:58:51ZComparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.1932-620310.1371/journal.pone.0107807https://doaj.org/article/547691fb82634d8ab56e813ca8fe2d3e2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107807https://doaj.org/toc/1932-6203It was found that antibodies (Abs) against myelin basic protein (MBP) are the major components of the antibody response in multiple sclerosis (MS) patients. We have recently shown that IgGs from sera of MS patients are active in the hydrolysis of MBP. However, in literature there are no available data concerning possible MBP-hydrolyzing Abs in cerebrospinal fluid (CSF) of MS patients. We have shown that the average content of IgGs in their sera is about 195-fold higher than that in their CSF. Here we have compared, for the first time, the average content of lambda- and kappa-IgGs as well as IgGs of four different subclasses (IgG1-IgG4) in CSF and sera of MS patients. The average relative content of lambda-IgGs and kappa -IgGs in the case of CSFs (8.0 and 92.0%) and sera (12.3 and 87.7%) are comparable, while IgG1, IgG2, IgG3, and IgG4: CSF - 40.4, 49.0, 8.2, and 2.5% of total IgGs, respectively and the sera - 53.6, 36.0, 5.6, and 4.8%, decreased in different order. Electrophoretically and immunologically homogeneous IgGs were obtained by sequential affinity chromatography of the CSF proteins on protein G-Sepharose and FPLC gel filtration. We present first evidence showing that IgGs from CSF efficiently hydrolyze MBP and that their average specific catalytic activity is unpredictably ∼54-fold higher than that of Abs from sera of the same MS patients. Some possible reasons of these findings are discussed. We suggest that anti-MBP abzymes of CSF may promote important neuropathologic mechanisms in this chronic inflammatory disorder and in MS pathogenesis development.Visilii B DoroninTaisiya A ParkhomenkoMassimiliano CastellazziMarina PadroniMichela PastoreValentina N BunevaEnrico GranieriGeorgy A NevinskyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107807 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Visilii B Doronin Taisiya A Parkhomenko Massimiliano Castellazzi Marina Padroni Michela Pastore Valentina N Buneva Enrico Granieri Georgy A Nevinsky Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
description	It was found that antibodies (Abs) against myelin basic protein (MBP) are the major components of the antibody response in multiple sclerosis (MS) patients. We have recently shown that IgGs from sera of MS patients are active in the hydrolysis of MBP. However, in literature there are no available data concerning possible MBP-hydrolyzing Abs in cerebrospinal fluid (CSF) of MS patients. We have shown that the average content of IgGs in their sera is about 195-fold higher than that in their CSF. Here we have compared, for the first time, the average content of lambda- and kappa-IgGs as well as IgGs of four different subclasses (IgG1-IgG4) in CSF and sera of MS patients. The average relative content of lambda-IgGs and kappa -IgGs in the case of CSFs (8.0 and 92.0%) and sera (12.3 and 87.7%) are comparable, while IgG1, IgG2, IgG3, and IgG4: CSF - 40.4, 49.0, 8.2, and 2.5% of total IgGs, respectively and the sera - 53.6, 36.0, 5.6, and 4.8%, decreased in different order. Electrophoretically and immunologically homogeneous IgGs were obtained by sequential affinity chromatography of the CSF proteins on protein G-Sepharose and FPLC gel filtration. We present first evidence showing that IgGs from CSF efficiently hydrolyze MBP and that their average specific catalytic activity is unpredictably ∼54-fold higher than that of Abs from sera of the same MS patients. Some possible reasons of these findings are discussed. We suggest that anti-MBP abzymes of CSF may promote important neuropathologic mechanisms in this chronic inflammatory disorder and in MS pathogenesis development.
format	article
author	Visilii B Doronin Taisiya A Parkhomenko Massimiliano Castellazzi Marina Padroni Michela Pastore Valentina N Buneva Enrico Granieri Georgy A Nevinsky
author_facet	Visilii B Doronin Taisiya A Parkhomenko Massimiliano Castellazzi Marina Padroni Michela Pastore Valentina N Buneva Enrico Granieri Georgy A Nevinsky
author_sort	Visilii B Doronin
title	Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_short	Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_full	Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_fullStr	Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_full_unstemmed	Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
title_sort	comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/547691fb82634d8ab56e813ca8fe2d3e
work_keys_str_mv	AT visiliibdoronin comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT taisiyaaparkhomenko comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT massimilianocastellazzi comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT marinapadroni comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT michelapastore comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT valentinanbuneva comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT enricogranieri comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis AT georgyanevinsky comparisonofantibodieshydrolyzingmyelinbasicproteinfromthecerebrospinalfluidandserumofpatientswithmultiplesclerosis
_version_	1718414337400373248

Comparison of antibodies hydrolyzing myelin basic protein from the cerebrospinal fluid and serum of patients with multiple sclerosis.

Ejemplares similares