Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy

Abstract Platelets are small anucleate blood cells involved in haemostasis. Platelet activation, caused by agonists such as thrombin or by contact with the extracellular matrix, leads to platelet adhesion, aggregation, and coagulation. Activated platelets undergo shape changes, adhere, and spread at...

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Autores principales:	Jan Seifert, Johannes Rheinlaender, Florian Lang, Meinrad Gawaz, Tilman E. Schäffer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5477d2597db4471d984c3e84336a4ac7
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spelling	oai:doaj.org-article:5477d2597db4471d984c3e84336a4ac72021-12-02T16:06:14ZThrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy10.1038/s41598-017-04999-62045-2322https://doaj.org/article/5477d2597db4471d984c3e84336a4ac72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04999-6https://doaj.org/toc/2045-2322Abstract Platelets are small anucleate blood cells involved in haemostasis. Platelet activation, caused by agonists such as thrombin or by contact with the extracellular matrix, leads to platelet adhesion, aggregation, and coagulation. Activated platelets undergo shape changes, adhere, and spread at the site of injury to form a blood clot. We investigated the morphology and morphological dynamics of human platelets after complete spreading using fast scanning ion conductance microscopy (SICM). In contrast to unstimulated platelets, thrombin-stimulated platelets showed increased morphological activity after spreading and exhibited dynamic morphological changes in the form of wave-like movements of the lamellipodium and dynamic protrusions on the platelet body. The increase in morphological activity was dependent on thrombin concentration. No increase in activity was observed following exposure to other activation agonists or during contact-induced activation. Inhibition of actin polymerization and inhibition of dynein significantly decreased the activity of thrombin-stimulated platelets. Our data suggest that these morphological dynamics after spreading are thrombin-specific and might play a role in coagulation and blood clot formation.Jan SeifertJohannes RheinlaenderFlorian LangMeinrad GawazTilman E. SchäfferNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jan Seifert Johannes Rheinlaender Florian Lang Meinrad Gawaz Tilman E. Schäffer Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
description	Abstract Platelets are small anucleate blood cells involved in haemostasis. Platelet activation, caused by agonists such as thrombin or by contact with the extracellular matrix, leads to platelet adhesion, aggregation, and coagulation. Activated platelets undergo shape changes, adhere, and spread at the site of injury to form a blood clot. We investigated the morphology and morphological dynamics of human platelets after complete spreading using fast scanning ion conductance microscopy (SICM). In contrast to unstimulated platelets, thrombin-stimulated platelets showed increased morphological activity after spreading and exhibited dynamic morphological changes in the form of wave-like movements of the lamellipodium and dynamic protrusions on the platelet body. The increase in morphological activity was dependent on thrombin concentration. No increase in activity was observed following exposure to other activation agonists or during contact-induced activation. Inhibition of actin polymerization and inhibition of dynein significantly decreased the activity of thrombin-stimulated platelets. Our data suggest that these morphological dynamics after spreading are thrombin-specific and might play a role in coagulation and blood clot formation.
format	article
author	Jan Seifert Johannes Rheinlaender Florian Lang Meinrad Gawaz Tilman E. Schäffer
author_facet	Jan Seifert Johannes Rheinlaender Florian Lang Meinrad Gawaz Tilman E. Schäffer
author_sort	Jan Seifert
title	Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
title_short	Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
title_full	Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
title_fullStr	Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
title_full_unstemmed	Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
title_sort	thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5477d2597db4471d984c3e84336a4ac7
work_keys_str_mv	AT janseifert thrombininducedcytoskeletondynamicsinspreadhumanplateletsobservedwithfastscanningionconductancemicroscopy AT johannesrheinlaender thrombininducedcytoskeletondynamicsinspreadhumanplateletsobservedwithfastscanningionconductancemicroscopy AT florianlang thrombininducedcytoskeletondynamicsinspreadhumanplateletsobservedwithfastscanningionconductancemicroscopy AT meinradgawaz thrombininducedcytoskeletondynamicsinspreadhumanplateletsobservedwithfastscanningionconductancemicroscopy AT tilmaneschaffer thrombininducedcytoskeletondynamicsinspreadhumanplateletsobservedwithfastscanningionconductancemicroscopy
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Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy

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