Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae

Abstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) used in biological control of agricultural pest insects. It enters the hemocoel of its host via the intestinal tract and releases its symbiotic bacterium Xenorhabdus nematophila. In order to improve our knowledge about the physio...

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Autores principales:	Louise Huot, Simon George, Pierre-Alain Girard, Dany Severac, Nicolas Nègre, Bernard Duvic
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/548374f4997747c79dafa34d8f89a9ec
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spelling	oai:doaj.org-article:548374f4997747c79dafa34d8f89a9ec2021-12-02T15:10:03ZSpodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae10.1038/s41598-019-49410-82045-2322https://doaj.org/article/548374f4997747c79dafa34d8f89a9ec2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49410-8https://doaj.org/toc/2045-2322Abstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) used in biological control of agricultural pest insects. It enters the hemocoel of its host via the intestinal tract and releases its symbiotic bacterium Xenorhabdus nematophila. In order to improve our knowledge about the physiological responses of its different hosts, we examined the transcriptional responses to EPN infestation of the fat body, the hemocytes and the midgut in the lepidopteran pest Spodoptera frugiperda. The tissues poorly respond to the infestation at an early time post-infestation of 8 h with only 5 genes differentially expressed in the fat body of the caterpillars. Strong transcriptional responses are observed at a later time point of 15 h post-infestation in all three tissues. Few genes are differentially expressed in the midgut but tissue-specific panels of induced metalloprotease inhibitors, immune receptors and antimicrobial peptides together with several uncharacterized genes are up-regulated in the fat body and the hemocytes. Among the most up-regulated genes, we identified new potential immune effectors, unique to Lepidoptera, which show homology with bacterial genes of unknown function. Altogether, these results pave the way for further functional studies of the responsive genes’ involvement in the interaction with the EPN.Louise HuotSimon GeorgePierre-Alain GirardDany SeveracNicolas NègreBernard DuvicNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Louise Huot Simon George Pierre-Alain Girard Dany Severac Nicolas Nègre Bernard Duvic Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
description	Abstract Steinernema carpocapsae is an entomopathogenic nematode (EPN) used in biological control of agricultural pest insects. It enters the hemocoel of its host via the intestinal tract and releases its symbiotic bacterium Xenorhabdus nematophila. In order to improve our knowledge about the physiological responses of its different hosts, we examined the transcriptional responses to EPN infestation of the fat body, the hemocytes and the midgut in the lepidopteran pest Spodoptera frugiperda. The tissues poorly respond to the infestation at an early time post-infestation of 8 h with only 5 genes differentially expressed in the fat body of the caterpillars. Strong transcriptional responses are observed at a later time point of 15 h post-infestation in all three tissues. Few genes are differentially expressed in the midgut but tissue-specific panels of induced metalloprotease inhibitors, immune receptors and antimicrobial peptides together with several uncharacterized genes are up-regulated in the fat body and the hemocytes. Among the most up-regulated genes, we identified new potential immune effectors, unique to Lepidoptera, which show homology with bacterial genes of unknown function. Altogether, these results pave the way for further functional studies of the responsive genes’ involvement in the interaction with the EPN.
format	article
author	Louise Huot Simon George Pierre-Alain Girard Dany Severac Nicolas Nègre Bernard Duvic
author_facet	Louise Huot Simon George Pierre-Alain Girard Dany Severac Nicolas Nègre Bernard Duvic
author_sort	Louise Huot
title	Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
title_short	Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
title_full	Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
title_fullStr	Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
title_full_unstemmed	Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae
title_sort	spodoptera frugiperda transcriptional response to infestation by steinernema carpocapsae
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/548374f4997747c79dafa34d8f89a9ec
work_keys_str_mv	AT louisehuot spodopterafrugiperdatranscriptionalresponsetoinfestationbysteinernemacarpocapsae AT simongeorge spodopterafrugiperdatranscriptionalresponsetoinfestationbysteinernemacarpocapsae AT pierrealaingirard spodopterafrugiperdatranscriptionalresponsetoinfestationbysteinernemacarpocapsae AT danyseverac spodopterafrugiperdatranscriptionalresponsetoinfestationbysteinernemacarpocapsae AT nicolasnegre spodopterafrugiperdatranscriptionalresponsetoinfestationbysteinernemacarpocapsae AT bernardduvic spodopterafrugiperdatranscriptionalresponsetoinfestationbysteinernemacarpocapsae
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Spodoptera frugiperda transcriptional response to infestation by Steinernema carpocapsae

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