Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry

Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors worldwide. The clinical treatment of ccRCC is strongly associated with the tumor microenvironment (TME). Identifying potential markers of ccRCC is important to improve prognosis. Therefore, in the present study, the l...

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Autores principales:	Di Han, Zhongjie Yu, Hong Zhang, Haipeng Liu, Bin Wang, Donmeng Qian
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	immunoglobulin hydroxysteroid 11-beta dehydrogenase-1 clear cell renal cell carcinoma immune infiltration prognosis tumor microenvironment Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5488ac8dda3d45879fd8d029a5f637f5
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spelling	oai:doaj.org-article:5488ac8dda3d45879fd8d029a5f637f52021-12-01T14:41:00ZMicroenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry2165-59792165-598710.1080/21655979.2021.1994908https://doaj.org/article/5488ac8dda3d45879fd8d029a5f637f52021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1994908https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors worldwide. The clinical treatment of ccRCC is strongly associated with the tumor microenvironment (TME). Identifying potential markers of ccRCC is important to improve prognosis. Therefore, in the present study, the levels of immune/stromal components and the proportion of tumor-infiltrating immune cells (TIICs) were determined in 611 ccRCC samples using the ESTIMATE and CIBERSORT analytical tools. Subsequently, hydroxysteroid 11-beta dehydrogenase-1 (HSD11B1) was identified by univariate Cox regression analysis, protein-protein interaction (PPI) networks and clinical survival analysis to be associated with ccRCC prognosis. At the same time, the abundance of HSD11B1 increased significantly in ccRCC was verified by western blotting, RT‑qPCR and immunostaining analysis. Furthermore, Gene Set Enrichment Analysis (GSEA) and TME suggested that HSD11B1 was involved in TME immune-related status. Taken together, the results of the present study demonstrated that HSD11B1 is a potential prognostic biomarker associated with immune cell infiltration in ccRCC.Di HanZhongjie YuHong ZhangHaipeng LiuBin WangDonmeng QianTaylor & Francis Grouparticleimmunoglobulin hydroxysteroid 11-beta dehydrogenase-1clear cell renal cell carcinomaimmune infiltrationprognosistumor microenvironmentBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 10891-10904 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunoglobulin hydroxysteroid 11-beta dehydrogenase-1 clear cell renal cell carcinoma immune infiltration prognosis tumor microenvironment Biotechnology TP248.13-248.65
spellingShingle	immunoglobulin hydroxysteroid 11-beta dehydrogenase-1 clear cell renal cell carcinoma immune infiltration prognosis tumor microenvironment Biotechnology TP248.13-248.65 Di Han Zhongjie Yu Hong Zhang Haipeng Liu Bin Wang Donmeng Qian Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry
description	Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors worldwide. The clinical treatment of ccRCC is strongly associated with the tumor microenvironment (TME). Identifying potential markers of ccRCC is important to improve prognosis. Therefore, in the present study, the levels of immune/stromal components and the proportion of tumor-infiltrating immune cells (TIICs) were determined in 611 ccRCC samples using the ESTIMATE and CIBERSORT analytical tools. Subsequently, hydroxysteroid 11-beta dehydrogenase-1 (HSD11B1) was identified by univariate Cox regression analysis, protein-protein interaction (PPI) networks and clinical survival analysis to be associated with ccRCC prognosis. At the same time, the abundance of HSD11B1 increased significantly in ccRCC was verified by western blotting, RT‑qPCR and immunostaining analysis. Furthermore, Gene Set Enrichment Analysis (GSEA) and TME suggested that HSD11B1 was involved in TME immune-related status. Taken together, the results of the present study demonstrated that HSD11B1 is a potential prognostic biomarker associated with immune cell infiltration in ccRCC.
format	article
author	Di Han Zhongjie Yu Hong Zhang Haipeng Liu Bin Wang Donmeng Qian
author_facet	Di Han Zhongjie Yu Hong Zhang Haipeng Liu Bin Wang Donmeng Qian
author_sort	Di Han
title	Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry
title_short	Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry
title_full	Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry
title_fullStr	Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry
title_full_unstemmed	Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry
title_sort	microenvironment-associated gene hsd11b1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on tcga, rt‑qpcr, western blotting, and immunohistochemistry
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/5488ac8dda3d45879fd8d029a5f637f5
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Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry

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