Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>

The SGT1 protein is highly expressed in the mammalian brain, particularly in neurons of the hippocampus and cortex, and in Purkinje cells of the cerebellum. There are literature data indicating that the protein may be involved in pathogenesis of neurodegenerative disorders such as Parkinson’s diseas...

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Autores principales:	Anastasiia Bohush, Agnieszka Góral, Małgorzata Sierant, Barbara Nawrot, Wiesława Leśniak, Anna Filipek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	α-synuclein SGT1 subcellular localization Parkinson’s disease <i>PINK1</i> <i>PARK9</i> Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/548af5e5c60744fcb107787e249addc7
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spelling	oai:doaj.org-article:548af5e5c60744fcb107787e249addc72021-11-25T16:53:41ZSgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>10.3390/biom111116752218-273Xhttps://doaj.org/article/548af5e5c60744fcb107787e249addc72021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1675https://doaj.org/toc/2218-273XThe SGT1 protein is highly expressed in the mammalian brain, particularly in neurons of the hippocampus and cortex, and in Purkinje cells of the cerebellum. There are literature data indicating that the protein may be involved in pathogenesis of neurodegenerative disorders such as Parkinson’s disease (PD). In the present work we have found that SGT1 protected cells from the toxicity of rotenone, an agent that evokes behavioral and histopathological symptoms of PD. To gain more insight into the possible mechanism underlying the protective action of SGT1 we looked at α-synuclein subcellular distribution in HEK293 cells with an altered SGT1 level. By immunofluorescent staining we have found that in HEK293 cells overexpressing SGT1 α-synuclein was mainly localized in the cytoplasm while in control cells it was present in the nucleus. Accordingly, when SGT1 expression was silenced, α-synuclein was predominantly present in the nucleus. These results were then confirmed by subcellular fractionation and Western blot analysis. Moreover, we have found that altered level of SGT1 in HEK293 cells influenced the expression of PD related genes, <i>PINK1</i> and <i>PARK9.</i> Altogether, our results point to SGT1 as an important factor that might be involved in the pathogenesis of Parkinson’s disease (PD).Anastasiia BohushAgnieszka GóralMałgorzata SierantBarbara NawrotWiesława LeśniakAnna FilipekMDPI AGarticleα-synucleinSGT1subcellular localizationParkinson’s disease<i>PINK1</i><i>PARK9</i>MicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1675, p 1675 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	α-synuclein SGT1 subcellular localization Parkinson’s disease <i>PINK1</i> <i>PARK9</i> Microbiology QR1-502
spellingShingle	α-synuclein SGT1 subcellular localization Parkinson’s disease <i>PINK1</i> <i>PARK9</i> Microbiology QR1-502 Anastasiia Bohush Agnieszka Góral Małgorzata Sierant Barbara Nawrot Wiesława Leśniak Anna Filipek Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>
description	The SGT1 protein is highly expressed in the mammalian brain, particularly in neurons of the hippocampus and cortex, and in Purkinje cells of the cerebellum. There are literature data indicating that the protein may be involved in pathogenesis of neurodegenerative disorders such as Parkinson’s disease (PD). In the present work we have found that SGT1 protected cells from the toxicity of rotenone, an agent that evokes behavioral and histopathological symptoms of PD. To gain more insight into the possible mechanism underlying the protective action of SGT1 we looked at α-synuclein subcellular distribution in HEK293 cells with an altered SGT1 level. By immunofluorescent staining we have found that in HEK293 cells overexpressing SGT1 α-synuclein was mainly localized in the cytoplasm while in control cells it was present in the nucleus. Accordingly, when SGT1 expression was silenced, α-synuclein was predominantly present in the nucleus. These results were then confirmed by subcellular fractionation and Western blot analysis. Moreover, we have found that altered level of SGT1 in HEK293 cells influenced the expression of PD related genes, <i>PINK1</i> and <i>PARK9.</i> Altogether, our results point to SGT1 as an important factor that might be involved in the pathogenesis of Parkinson’s disease (PD).
format	article
author	Anastasiia Bohush Agnieszka Góral Małgorzata Sierant Barbara Nawrot Wiesława Leśniak Anna Filipek
author_facet	Anastasiia Bohush Agnieszka Góral Małgorzata Sierant Barbara Nawrot Wiesława Leśniak Anna Filipek
author_sort	Anastasiia Bohush
title	Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>
title_short	Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>
title_full	Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>
title_fullStr	Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>
title_full_unstemmed	Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>
title_sort	sgt1 regulates α-synuclein subcellular localization and expression of parkinson’s disease related genes, <i>pink1</i> and <i>park9</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/548af5e5c60744fcb107787e249addc7
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Sgt1 Regulates α-Synuclein Subcellular Localization and Expression of Parkinson’s Disease Related Genes, <i>PINK1</i> and <i>PARK9</i>

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