In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN<sub>1</sub>)&l...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/548ba9faf6054b72a982f19a387fc839 |
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| Sumario: | Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN<sub>1</sub>)<sub>2</sub>]ClO<sub>4</sub>, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in <i>Vibrio harveyi</i> BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory concentration (IC<sub>50</sub>) determination. Antibiofilm capacity of copper (I) complex was analyzed by different concentrations of IC<sub>50</sub> values. The results showed that the sub-IC<sub>50</sub> concentration, 12.6 µg/mL of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the [Cu(NN<sub>1</sub>)<sub>2</sub>]ClO<sub>4</sub> complex affected the biofilm structure. Therefore, the copper (I) complex is effective as an antibiofilm compound in <i>V. harveyi</i> BB170. |
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