In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation

In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation

Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN<sub>1</sub>)&l...

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Autores principales: Sarita Soto-Aguilera, Brenda Modak, Maialen Aldabaldetrecu, Carla P. Lozano, Juan Guerrero, Claudia Lefimil, Mick Parra
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
biofilm
antibacterial activity
antibiofilm activity
copper (I) complex
coumarin
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/548ba9faf6054b72a982f19a387fc839
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spelling oai:doaj.org-article:548ba9faf6054b72a982f19a387fc8392021-11-25T18:24:46ZIn Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation10.3390/microorganisms91122732076-2607https://doaj.org/article/548ba9faf6054b72a982f19a387fc8392021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2273https://doaj.org/toc/2076-2607Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN<sub>1</sub>)<sub>2</sub>]ClO<sub>4</sub>, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in <i>Vibrio harveyi</i> BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory concentration (IC<sub>50</sub>) determination. Antibiofilm capacity of copper (I) complex was analyzed by different concentrations of IC<sub>50</sub> values. The results showed that the sub-IC<sub>50</sub> concentration, 12.6 µg/mL of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the [Cu(NN<sub>1</sub>)<sub>2</sub>]ClO<sub>4</sub> complex affected the biofilm structure. Therefore, the copper (I) complex is effective as an antibiofilm compound in <i>V. harveyi</i> BB170.Sarita Soto-AguileraBrenda ModakMaialen AldabaldetrecuCarla P. LozanoJuan GuerreroClaudia LefimilMick ParraMDPI AGarticlebiofilmantibacterial activityantibiofilm activitycopper (I) complexcoumarinBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2273, p 2273 (2021)
institution DOAJ
collection DOAJ
language EN
topic biofilm
antibacterial activity
antibiofilm activity
copper (I) complex
coumarin
Biology (General)
QH301-705.5
spellingShingle biofilm
antibacterial activity
antibiofilm activity
copper (I) complex
coumarin
Biology (General)
QH301-705.5
Sarita Soto-Aguilera
Brenda Modak
Maialen Aldabaldetrecu
Carla P. Lozano
Juan Guerrero
Claudia Lefimil
Mick Parra
In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
description Biofilm formation in pathogenic bacteria is an important factor of resistance to antimicrobial treatments, allowing them to survive for a long time in their hosts. In the search for new antibiofilm agents, in this work we report the activity of a copper (I) complex, [Cu(NN<sub>1</sub>)<sub>2</sub>]ClO<sub>4</sub>, synthesized with Cu (I) and NN1, an imine ligand 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one, a derivate of natural compound coumarin. The antibacterial and antibiofilm capacity was evaluated in <i>Vibrio harveyi</i> BB170 used as model bacteria. Antibacterial activity was measured in vitro by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and half-maximal inhibitory concentration (IC<sub>50</sub>) determination. Antibiofilm capacity of copper (I) complex was analyzed by different concentrations of IC<sub>50</sub> values. The results showed that the sub-IC<sub>50</sub> concentration, 12.6 µg/mL of the copper (I) complex, was able to reduce biofilm formation by more than 75%, and bacterial viability was reduced by 50%. Inverted and confocal laser scanning microscopy showed that the [Cu(NN<sub>1</sub>)<sub>2</sub>]ClO<sub>4</sub> complex affected the biofilm structure. Therefore, the copper (I) complex is effective as an antibiofilm compound in <i>V. harveyi</i> BB170.
format article
author Sarita Soto-Aguilera
Brenda Modak
Maialen Aldabaldetrecu
Carla P. Lozano
Juan Guerrero
Claudia Lefimil
Mick Parra
author_facet Sarita Soto-Aguilera
Brenda Modak
Maialen Aldabaldetrecu
Carla P. Lozano
Juan Guerrero
Claudia Lefimil
Mick Parra
author_sort Sarita Soto-Aguilera
title In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
title_short In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
title_full In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
title_fullStr In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
title_full_unstemmed In Vitro Effect of Copper (I) Complex [Cu(NN<sub>1</sub>)<sub>2</sub>](ClO<sub>4</sub>) on <i>Vibrio harveyi</i> BB170 Biofilm Formation
title_sort in vitro effect of copper (i) complex [cu(nn<sub>1</sub>)<sub>2</sub>](clo<sub>4</sub>) on <i>vibrio harveyi</i> bb170 biofilm formation
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/548ba9faf6054b72a982f19a387fc839
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