Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.

<h4>Introduction</h4>The etiology of diverticulosis is still poorly understood. However, in patients with diverticulitis, markers of mucosal inflammation and microbiota alterations have been found. The aim of this study was to evaluate potential differences of the gut microbiota composit...

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Autores principales:	Tessel M van Rossen, Rogier E Ooijevaar, Johan Ph Kuyvenhoven, Anat Eck, Herman Bril, René Buijsman, Marja A Boermeester, Hein B A C Stockmann, Niels de Korte, Andries E Budding
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/548d1f0584034efd9ce33e3cd17c91ca
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spelling	oai:doaj.org-article:548d1f0584034efd9ce33e3cd17c91ca2021-12-02T20:08:29ZMicrobiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.1932-620310.1371/journal.pone.0256657https://doaj.org/article/548d1f0584034efd9ce33e3cd17c91ca2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256657https://doaj.org/toc/1932-6203<h4>Introduction</h4>The etiology of diverticulosis is still poorly understood. However, in patients with diverticulitis, markers of mucosal inflammation and microbiota alterations have been found. The aim of this study was to evaluate potential differences of the gut microbiota composition and mucosal immunity between patients with asymptomatic diverticulosis and controls.<h4>Methods</h4>We performed a prospective study on patients who underwent routine colonoscopy for causes not related to diverticular disease or inflammatory bowel disease. Participants were grouped based on the presence or absence of diverticula. Mucosal biopsies were obtained from the sigmoid and transverse colon. Microbiota composition was analyzed with IS-pro, a 16S-23S based bacterial profiling technique. To predict if patients belonged to the asymptomatic diverticulosis or control group a partial least squares discriminant analysis (PLS-DA) regression model was used. Inflammation was assessed by neutrophil and lymphocyte counts within the taken biopsies.<h4>Results</h4>Forty-three patients were enrolled. Intestinal microbiota profiles were highly similar within individuals for all phyla. Between individuals, microbiota profiles differed substantially but regardless of the presence (n = 19) of absence (n = 24) of diverticula. Microbiota diversity in both sigmoid and transverse colon was similar in all participants. We were not able to differentiate between diverticulosis patients and controls with a PLS-DA model. Mucosal lymphocyte counts were comparable among both groups; no neutrophils were detected in any of the studied biopsies.<h4>Conclusions</h4>Microbiota composition and inflammatory markers were comparable among asymptomatic diverticulosis patients and controls. This suggests that the gut microbiota and mucosal inflammation do not play a major role in the pathogenesis of diverticula formation.Tessel M van RossenRogier E OoijevaarJohan Ph KuyvenhovenAnat EckHerman BrilRené BuijsmanMarja A BoermeesterHein B A C StockmannNiels de KorteAndries E BuddingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256657 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tessel M van Rossen Rogier E Ooijevaar Johan Ph Kuyvenhoven Anat Eck Herman Bril René Buijsman Marja A Boermeester Hein B A C Stockmann Niels de Korte Andries E Budding Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
description	<h4>Introduction</h4>The etiology of diverticulosis is still poorly understood. However, in patients with diverticulitis, markers of mucosal inflammation and microbiota alterations have been found. The aim of this study was to evaluate potential differences of the gut microbiota composition and mucosal immunity between patients with asymptomatic diverticulosis and controls.<h4>Methods</h4>We performed a prospective study on patients who underwent routine colonoscopy for causes not related to diverticular disease or inflammatory bowel disease. Participants were grouped based on the presence or absence of diverticula. Mucosal biopsies were obtained from the sigmoid and transverse colon. Microbiota composition was analyzed with IS-pro, a 16S-23S based bacterial profiling technique. To predict if patients belonged to the asymptomatic diverticulosis or control group a partial least squares discriminant analysis (PLS-DA) regression model was used. Inflammation was assessed by neutrophil and lymphocyte counts within the taken biopsies.<h4>Results</h4>Forty-three patients were enrolled. Intestinal microbiota profiles were highly similar within individuals for all phyla. Between individuals, microbiota profiles differed substantially but regardless of the presence (n = 19) of absence (n = 24) of diverticula. Microbiota diversity in both sigmoid and transverse colon was similar in all participants. We were not able to differentiate between diverticulosis patients and controls with a PLS-DA model. Mucosal lymphocyte counts were comparable among both groups; no neutrophils were detected in any of the studied biopsies.<h4>Conclusions</h4>Microbiota composition and inflammatory markers were comparable among asymptomatic diverticulosis patients and controls. This suggests that the gut microbiota and mucosal inflammation do not play a major role in the pathogenesis of diverticula formation.
format	article
author	Tessel M van Rossen Rogier E Ooijevaar Johan Ph Kuyvenhoven Anat Eck Herman Bril René Buijsman Marja A Boermeester Hein B A C Stockmann Niels de Korte Andries E Budding
author_facet	Tessel M van Rossen Rogier E Ooijevaar Johan Ph Kuyvenhoven Anat Eck Herman Bril René Buijsman Marja A Boermeester Hein B A C Stockmann Niels de Korte Andries E Budding
author_sort	Tessel M van Rossen
title	Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
title_short	Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
title_full	Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
title_fullStr	Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
title_full_unstemmed	Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
title_sort	microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/548d1f0584034efd9ce33e3cd17c91ca
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Microbiota composition and mucosal immunity in patients with asymptomatic diverticulosis and controls.

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