Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.

<h4>Background</h4>Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.<h4>Methodology/principal findings</h4>We optimized a Populus leaf mesophyll pro...

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Autores principales:	Jianjun Guo, Jennifer L Morrell-Falvey, Jessy L Labbé, Wellington Muchero, Udaya C Kalluri, Gerald A Tuskan, Jin-Gui Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/548dbedd4b454633b8dc8bbd0a34e888
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spelling	oai:doaj.org-article:548dbedd4b454633b8dc8bbd0a34e8882021-11-18T07:05:41ZHighly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.1932-620310.1371/journal.pone.0044908https://doaj.org/article/548dbedd4b454633b8dc8bbd0a34e8882012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028673/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.<h4>Methodology/principal findings</h4>We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts are able to respond to hormonal stimuli and that a series of organelle markers are correctly localized in the Populus protoplasts. Furthermore, we showed that the Populus protoplast transient expression system is suitable for studying protein-protein interaction, gene activation, and cellular signaling events.<h4>Conclusions/significance</h4>This study established a method for efficient isolation of protoplasts from Populus leaf and demonstrated the efficacy of using Populus protoplast transient expression assays as an in vivo system to characterize genes and pathways.Jianjun GuoJennifer L Morrell-FalveyJessy L LabbéWellington MucheroUdaya C KalluriGerald A TuskanJin-Gui ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e44908 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jianjun Guo Jennifer L Morrell-Falvey Jessy L Labbé Wellington Muchero Udaya C Kalluri Gerald A Tuskan Jin-Gui Chen Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
description	<h4>Background</h4>Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function.<h4>Methodology/principal findings</h4>We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts are able to respond to hormonal stimuli and that a series of organelle markers are correctly localized in the Populus protoplasts. Furthermore, we showed that the Populus protoplast transient expression system is suitable for studying protein-protein interaction, gene activation, and cellular signaling events.<h4>Conclusions/significance</h4>This study established a method for efficient isolation of protoplasts from Populus leaf and demonstrated the efficacy of using Populus protoplast transient expression assays as an in vivo system to characterize genes and pathways.
format	article
author	Jianjun Guo Jennifer L Morrell-Falvey Jessy L Labbé Wellington Muchero Udaya C Kalluri Gerald A Tuskan Jin-Gui Chen
author_facet	Jianjun Guo Jennifer L Morrell-Falvey Jessy L Labbé Wellington Muchero Udaya C Kalluri Gerald A Tuskan Jin-Gui Chen
author_sort	Jianjun Guo
title	Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
title_short	Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
title_full	Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
title_fullStr	Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
title_full_unstemmed	Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.
title_sort	highly efficient isolation of populus mesophyll protoplasts and its application in transient expression assays.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/548dbedd4b454633b8dc8bbd0a34e888
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Highly efficient isolation of Populus mesophyll protoplasts and its application in transient expression assays.

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