The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2.
<h4>Background</h4>The human immunodeficiency virus type 1 (HIV-1) p17 is a matrix protein involved in virus life's cycle. CXCR2 and Syndecan-2, the two major coreceptors for the p17 protein, are expressed in hepatic stellate cells (HSCs), a key cell type involved in matrix depositi...
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2014
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oai:doaj.org-article:5497e44e65cd497781d6ea41bc9c47de2021-11-18T08:23:09ZThe HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2.1932-620310.1371/journal.pone.0094798https://doaj.org/article/5497e44e65cd497781d6ea41bc9c47de2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24736615/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The human immunodeficiency virus type 1 (HIV-1) p17 is a matrix protein involved in virus life's cycle. CXCR2 and Syndecan-2, the two major coreceptors for the p17 protein, are expressed in hepatic stellate cells (HSCs), a key cell type involved in matrix deposition in liver fibrotic disorders.<h4>Aim</h4>In this report we have investigated the in vitro impact of p17 on HSCs transdifferentiation and function and underlying signaling pathways involved in these processes.<h4>Methods</h4>LX-2 cells, a human HSC line, and primary HSC were challenged with p17 and expressions of fibrogenic markers and of p17 receptors were assessed by qRT-PCR and Western blot. Downstream intracellular signaling pathways were evaluated with qRT-PCR and Western blot as well as after pre-treatment with specific pathway inhibitors.<h4>Results</h4>Exposure of LX2 cells to p17 increases their contractile force, reshapes the cytoskeleton fibers and upregulates the expression of transdifferentiation markers including αSMA, COL1α1 and endothelin-1 through the activation of Jak/STAT and Rho signaling pathways. These effects are lost in HSCs pre-incubated with a serum from HIV positive person who underwent a vaccination with a p17 peptide. Confocal laser microscopy studies demonstrates that CXCR2 and syndecan-2 co-associate at the plasma membrane after exposure to p17. Immunostaining of HIV/HCV liver biopsies from co-infected patients reveals that the progression of liver fibrosis correlates with a reduced expression of CXCR2.<h4>Conclusions</h4>The HIV matrix protein p17 is pro-fibrogenic through its interactions both with CXCR2 and syndecan-2 on activated HSCs.Barbara RengaDaniela FrancisciElisabetta SchiaroliAdriana CarinoSabrina CiprianiClaudio D'AmoreAngelo SidoniRachele Del SordoIvana FerriMonica LucattelliBenedetta LunghiFranco BaldelliStefano FiorucciPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94798 (2014) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Barbara Renga Daniela Francisci Elisabetta Schiaroli Adriana Carino Sabrina Cipriani Claudio D'Amore Angelo Sidoni Rachele Del Sordo Ivana Ferri Monica Lucattelli Benedetta Lunghi Franco Baldelli Stefano Fiorucci The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2. |
| description |
<h4>Background</h4>The human immunodeficiency virus type 1 (HIV-1) p17 is a matrix protein involved in virus life's cycle. CXCR2 and Syndecan-2, the two major coreceptors for the p17 protein, are expressed in hepatic stellate cells (HSCs), a key cell type involved in matrix deposition in liver fibrotic disorders.<h4>Aim</h4>In this report we have investigated the in vitro impact of p17 on HSCs transdifferentiation and function and underlying signaling pathways involved in these processes.<h4>Methods</h4>LX-2 cells, a human HSC line, and primary HSC were challenged with p17 and expressions of fibrogenic markers and of p17 receptors were assessed by qRT-PCR and Western blot. Downstream intracellular signaling pathways were evaluated with qRT-PCR and Western blot as well as after pre-treatment with specific pathway inhibitors.<h4>Results</h4>Exposure of LX2 cells to p17 increases their contractile force, reshapes the cytoskeleton fibers and upregulates the expression of transdifferentiation markers including αSMA, COL1α1 and endothelin-1 through the activation of Jak/STAT and Rho signaling pathways. These effects are lost in HSCs pre-incubated with a serum from HIV positive person who underwent a vaccination with a p17 peptide. Confocal laser microscopy studies demonstrates that CXCR2 and syndecan-2 co-associate at the plasma membrane after exposure to p17. Immunostaining of HIV/HCV liver biopsies from co-infected patients reveals that the progression of liver fibrosis correlates with a reduced expression of CXCR2.<h4>Conclusions</h4>The HIV matrix protein p17 is pro-fibrogenic through its interactions both with CXCR2 and syndecan-2 on activated HSCs. |
| format |
article |
| author |
Barbara Renga Daniela Francisci Elisabetta Schiaroli Adriana Carino Sabrina Cipriani Claudio D'Amore Angelo Sidoni Rachele Del Sordo Ivana Ferri Monica Lucattelli Benedetta Lunghi Franco Baldelli Stefano Fiorucci |
| author_facet |
Barbara Renga Daniela Francisci Elisabetta Schiaroli Adriana Carino Sabrina Cipriani Claudio D'Amore Angelo Sidoni Rachele Del Sordo Ivana Ferri Monica Lucattelli Benedetta Lunghi Franco Baldelli Stefano Fiorucci |
| author_sort |
Barbara Renga |
| title |
The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2. |
| title_short |
The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2. |
| title_full |
The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2. |
| title_fullStr |
The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2. |
| title_full_unstemmed |
The HIV matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with CXCR2 and Syndecan-2. |
| title_sort |
hiv matrix protein p17 promotes the activation of human hepatic stellate cells through interactions with cxcr2 and syndecan-2. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/5497e44e65cd497781d6ea41bc9c47de |
| work_keys_str_mv |
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