In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection

Abstract MAIT cells have been shown to be activated upon several viral infections in a TCR-independent manner by responding to inflammatory cytokines secreted by antigen-presenting cells. Recently, a few studies have shown a similar activation of MAIT cells in response to severe acute respiratory co...

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Autores principales:	Satanay Hubrack, Maryam Ali Al-Nesf, Nourhen Agrebi, Christophe Raynaud, Mohammed Abu Khattab, Merlin Thomas, Tayseer Ibrahim, Salma Taha, Said Dermime, Maysaloun Merhi, Michal Kulinski, Martin Steinhoff, Patrick Tang, Bernice Lo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/549c712b924949f8b6e4d46b8a7dc804
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spelling	oai:doaj.org-article:549c712b924949f8b6e4d46b8a7dc8042021-12-02T16:14:46ZIn vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection10.1038/s41598-021-93536-72045-2322https://doaj.org/article/549c712b924949f8b6e4d46b8a7dc8042021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93536-7https://doaj.org/toc/2045-2322Abstract MAIT cells have been shown to be activated upon several viral infections in a TCR-independent manner by responding to inflammatory cytokines secreted by antigen-presenting cells. Recently, a few studies have shown a similar activation of MAIT cells in response to severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. In this study, we investigate the effect of SARS-CoV-2 infection on the frequency and phenotype of MAIT cells by flow cytometry, and we test in vitro stimulation conditions on the capacity to enhance or rescue the antiviral function of MAIT cells from patients with coronavirus disease 2019 (COVID-19). Our study, in agreement with recently published studies, confirmed the decline in MAIT cell frequency of hospitalized donors in comparison to healthy donors. MAIT cells of COVID-19 patients also had lower expression levels of TNF-alpha, perforin and granzyme B upon stimulation with IL-12 + IL-18. 24 h’ incubation with IL-7 successfully restored perforin expression levels in COVID-19 patients. Combined, our findings support the growing evidence that SARS-CoV-2 is dysregulating MAIT cells and that IL-7 treatment might improve their function, rendering them more effective in protecting the body against the virus.Satanay HubrackMaryam Ali Al-NesfNourhen AgrebiChristophe RaynaudMohammed Abu KhattabMerlin ThomasTayseer IbrahimSalma TahaSaid DermimeMaysaloun MerhiMichal KulinskiMartin SteinhoffPatrick TangBernice LoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satanay Hubrack Maryam Ali Al-Nesf Nourhen Agrebi Christophe Raynaud Mohammed Abu Khattab Merlin Thomas Tayseer Ibrahim Salma Taha Said Dermime Maysaloun Merhi Michal Kulinski Martin Steinhoff Patrick Tang Bernice Lo In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
description	Abstract MAIT cells have been shown to be activated upon several viral infections in a TCR-independent manner by responding to inflammatory cytokines secreted by antigen-presenting cells. Recently, a few studies have shown a similar activation of MAIT cells in response to severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. In this study, we investigate the effect of SARS-CoV-2 infection on the frequency and phenotype of MAIT cells by flow cytometry, and we test in vitro stimulation conditions on the capacity to enhance or rescue the antiviral function of MAIT cells from patients with coronavirus disease 2019 (COVID-19). Our study, in agreement with recently published studies, confirmed the decline in MAIT cell frequency of hospitalized donors in comparison to healthy donors. MAIT cells of COVID-19 patients also had lower expression levels of TNF-alpha, perforin and granzyme B upon stimulation with IL-12 + IL-18. 24 h’ incubation with IL-7 successfully restored perforin expression levels in COVID-19 patients. Combined, our findings support the growing evidence that SARS-CoV-2 is dysregulating MAIT cells and that IL-7 treatment might improve their function, rendering them more effective in protecting the body against the virus.
format	article
author	Satanay Hubrack Maryam Ali Al-Nesf Nourhen Agrebi Christophe Raynaud Mohammed Abu Khattab Merlin Thomas Tayseer Ibrahim Salma Taha Said Dermime Maysaloun Merhi Michal Kulinski Martin Steinhoff Patrick Tang Bernice Lo
author_facet	Satanay Hubrack Maryam Ali Al-Nesf Nourhen Agrebi Christophe Raynaud Mohammed Abu Khattab Merlin Thomas Tayseer Ibrahim Salma Taha Said Dermime Maysaloun Merhi Michal Kulinski Martin Steinhoff Patrick Tang Bernice Lo
author_sort	Satanay Hubrack
title	In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
title_short	In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
title_full	In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
title_fullStr	In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
title_full_unstemmed	In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
title_sort	in vitro interleukin-7 treatment partially rescues mait cell dysfunction caused by sars-cov-2 infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/549c712b924949f8b6e4d46b8a7dc804
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In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection

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