The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth

Adipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentra...

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Autores principales:	Zhanna Alekseevna Akopyan, Georgy Vladimirovich Sharonov, Tatiana Nikolaevna Kochegura, Natalya Fedorovna Il'yashenko, Igor Eduardovich Belyanko, Valentina Ivanovna Dimitrova, Andrey Evgen'evich Zotikov, Natalya Igorevna Kalinina, Elena Viktorovna Parfenova
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2011
Materias:	hyperglycemia angiogenesis mesenchymal stem cells growth factors Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/549fadb525d940bd80e45d1ac7c24ce9
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spelling	oai:doaj.org-article:549fadb525d940bd80e45d1ac7c24ce92021-11-14T09:00:16ZThe influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth2072-03512072-037810.14341/2072-0351-5631https://doaj.org/article/549fadb525d940bd80e45d1ac7c24ce92011-06-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/5631https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Adipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentration on functional activity in human adipose tissue. Materials and methods. Flow cytometry and real time PCR were used to study functional activity of cultured MSC from human adipose issue at highglucose concentration. Results. Prolonged (10-12 days) incubation at a high glucose concentration (25 mM) suppressed the ability of MSC to stimulate angiogenesis. Also,glucose modified expression of genes activating and inhibiting angiogenesis but had no effect on MSC proliferation and apoptosis. Conclusion. High glucose concentration suppresses angiogenic activity of MSC in adipose tissue; it may account for incomplete restoration of bloodflow in diabetic patients.Zhanna Alekseevna AkopyanGeorgy Vladimirovich SharonovTatiana Nikolaevna KocheguraNatalya Fedorovna Il'yashenkoIgor Eduardovich BelyankoValentina Ivanovna DimitrovaAndrey Evgen'evich ZotikovNatalya Igorevna KalininaElena Viktorovna ParfenovaEndocrinology Research Centrearticlehyperglycemiaangiogenesismesenchymal stem cellsgrowth factorsNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 14, Iss 2, Pp 32-35 (2011)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	hyperglycemia angiogenesis mesenchymal stem cells growth factors Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	hyperglycemia angiogenesis mesenchymal stem cells growth factors Nutritional diseases. Deficiency diseases RC620-627 Zhanna Alekseevna Akopyan Georgy Vladimirovich Sharonov Tatiana Nikolaevna Kochegura Natalya Fedorovna Il'yashenko Igor Eduardovich Belyanko Valentina Ivanovna Dimitrova Andrey Evgen'evich Zotikov Natalya Igorevna Kalinina Elena Viktorovna Parfenova The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
description	Adipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentration on functional activity in human adipose tissue. Materials and methods. Flow cytometry and real time PCR were used to study functional activity of cultured MSC from human adipose issue at highglucose concentration. Results. Prolonged (10-12 days) incubation at a high glucose concentration (25 mM) suppressed the ability of MSC to stimulate angiogenesis. Also,glucose modified expression of genes activating and inhibiting angiogenesis but had no effect on MSC proliferation and apoptosis. Conclusion. High glucose concentration suppresses angiogenic activity of MSC in adipose tissue; it may account for incomplete restoration of bloodflow in diabetic patients.
format	article
author	Zhanna Alekseevna Akopyan Georgy Vladimirovich Sharonov Tatiana Nikolaevna Kochegura Natalya Fedorovna Il'yashenko Igor Eduardovich Belyanko Valentina Ivanovna Dimitrova Andrey Evgen'evich Zotikov Natalya Igorevna Kalinina Elena Viktorovna Parfenova
author_facet	Zhanna Alekseevna Akopyan Georgy Vladimirovich Sharonov Tatiana Nikolaevna Kochegura Natalya Fedorovna Il'yashenko Igor Eduardovich Belyanko Valentina Ivanovna Dimitrova Andrey Evgen'evich Zotikov Natalya Igorevna Kalinina Elena Viktorovna Parfenova
author_sort	Zhanna Alekseevna Akopyan
title	The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
title_short	The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
title_full	The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
title_fullStr	The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
title_full_unstemmed	The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
title_sort	influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
publisher	Endocrinology Research Centre
publishDate	2011
url	https://doaj.org/article/549fadb525d940bd80e45d1ac7c24ce9
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The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth

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