Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.

Dexamethasone (Dex) treated Severe Combined Immunodeficiency (SCID) mice were previously described as developing digestive adenocarcinoma after massive infection with Cryptosporidium parvum as soon as 45 days post-infection (P.I.). We aimed to determine the minimum number of oocysts capable of induc...

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Autores principales:	Sadia Benamrouz, Karine Guyot, Sophie Gazzola, Anthony Mouray, Thierry Chassat, Baptiste Delaire, Magali Chabé, Pierre Gosset, Eric Viscogliosi, Eduardo Dei-Cas, Colette Creusy, Valerie Conseil, Gabriela Certad
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/54aaf88fd309411f9a1f1b2b972d2f35
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spelling	oai:doaj.org-article:54aaf88fd309411f9a1f1b2b972d2f352021-11-18T08:05:13ZCryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.1932-620310.1371/journal.pone.0051232https://doaj.org/article/54aaf88fd309411f9a1f1b2b972d2f352012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23272093/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Dexamethasone (Dex) treated Severe Combined Immunodeficiency (SCID) mice were previously described as developing digestive adenocarcinoma after massive infection with Cryptosporidium parvum as soon as 45 days post-infection (P.I.). We aimed to determine the minimum number of oocysts capable of inducing infection and thereby gastrointestinal tumors in this model. Mice were challenged with calibrated oocyst suspensions containing intended doses of: 1, 10, 100 or 10(5) oocysts of C. parvum Iowa strain. All administered doses were infective for animals but increasing the oocyst challenge lead to an increase in mice infectivity (P = 0.01). Oocyst shedding was detected at 7 days P.I. after inoculation with more than 10 oocysts, and after 15 days in mice challenged with one oocyst. In groups challenged with lower inocula, parasite growth phase was significantly higher (P = 0.005) compared to mice inoculated with higher doses. After 45 days P.I. all groups of mice had a mean of oocyst shedding superior to 10,000 oocyst/g of feces. The most impressive observation of this study was the demonstration that C. parvum-induced digestive adenocarcinoma could be caused by infection with low doses of Cryptosporidium, even with only one oocyst: in mice inoculated with low doses, neoplastic lesions were detected as early as 45 days P.I. both in the stomach and ileo-caecal region, and these lesions could evolve in an invasive adenocarcinoma. These findings show a great amplification effect of parasites in mouse tissues after challenge with low doses as confirmed by quantitative PCR. The ability of C. parvum to infect mice with one oocyst and to develop digestive adenocarcinoma suggests that other mammalian species including humans could be also susceptible to this process, especially when they are severely immunocompromised.Sadia BenamrouzKarine GuyotSophie GazzolaAnthony MourayThierry ChassatBaptiste DelaireMagali ChabéPierre GossetEric ViscogliosiEduardo Dei-CasColette CreusyValerie ConseilGabriela CertadPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51232 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sadia Benamrouz Karine Guyot Sophie Gazzola Anthony Mouray Thierry Chassat Baptiste Delaire Magali Chabé Pierre Gosset Eric Viscogliosi Eduardo Dei-Cas Colette Creusy Valerie Conseil Gabriela Certad Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.
description	Dexamethasone (Dex) treated Severe Combined Immunodeficiency (SCID) mice were previously described as developing digestive adenocarcinoma after massive infection with Cryptosporidium parvum as soon as 45 days post-infection (P.I.). We aimed to determine the minimum number of oocysts capable of inducing infection and thereby gastrointestinal tumors in this model. Mice were challenged with calibrated oocyst suspensions containing intended doses of: 1, 10, 100 or 10(5) oocysts of C. parvum Iowa strain. All administered doses were infective for animals but increasing the oocyst challenge lead to an increase in mice infectivity (P = 0.01). Oocyst shedding was detected at 7 days P.I. after inoculation with more than 10 oocysts, and after 15 days in mice challenged with one oocyst. In groups challenged with lower inocula, parasite growth phase was significantly higher (P = 0.005) compared to mice inoculated with higher doses. After 45 days P.I. all groups of mice had a mean of oocyst shedding superior to 10,000 oocyst/g of feces. The most impressive observation of this study was the demonstration that C. parvum-induced digestive adenocarcinoma could be caused by infection with low doses of Cryptosporidium, even with only one oocyst: in mice inoculated with low doses, neoplastic lesions were detected as early as 45 days P.I. both in the stomach and ileo-caecal region, and these lesions could evolve in an invasive adenocarcinoma. These findings show a great amplification effect of parasites in mouse tissues after challenge with low doses as confirmed by quantitative PCR. The ability of C. parvum to infect mice with one oocyst and to develop digestive adenocarcinoma suggests that other mammalian species including humans could be also susceptible to this process, especially when they are severely immunocompromised.
format	article
author	Sadia Benamrouz Karine Guyot Sophie Gazzola Anthony Mouray Thierry Chassat Baptiste Delaire Magali Chabé Pierre Gosset Eric Viscogliosi Eduardo Dei-Cas Colette Creusy Valerie Conseil Gabriela Certad
author_facet	Sadia Benamrouz Karine Guyot Sophie Gazzola Anthony Mouray Thierry Chassat Baptiste Delaire Magali Chabé Pierre Gosset Eric Viscogliosi Eduardo Dei-Cas Colette Creusy Valerie Conseil Gabriela Certad
author_sort	Sadia Benamrouz
title	Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.
title_short	Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.
title_full	Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.
title_fullStr	Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.
title_full_unstemmed	Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.
title_sort	cryptosporidium parvum infection in scid mice infected with only one oocyst: qpcr assessment of parasite replication in tissues and development of digestive cancer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/54aaf88fd309411f9a1f1b2b972d2f35
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Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer.

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